Molecular and morphological characterization of Xylaria karsticola (Ascomycota) isolated from the fruiting body of Macrolepiota procera (Basidiomycota) from Bulgaria

The present study is the first to report Xylaria karsticola isolated from the basidiocarp of Macrolepiota procera (Basidiomycota), from Stara Planina Mountain, Bulgaria and second report for such species found in Europe. The fungal isolate was in vitro cultivated and the morphology was observed. It was primarily determined as a xylariaceous morphotype at the intragenus level, based on the evaluation of colony growth rate, color, and stromatic structure formation and was confirmed by unique conidiophores and conidia. The molecular identification of the isolate was performed by amplification of ITS1-5.8S-ITS2 region and the strain was identified as Xylaria karsticola with 97.57% of confidence. The obtained sequence was deposited in the GenBank database under the accession number MW996752 and in the National Bank of Industrial Microorganisms and Cell Cultures of Bulgaria under accession number NBIMCC 9097. The phylogenetic analysis of the isolate was also conducted by including 26 sequences obtained from different Xylaria isolates. Considering the phylogenetic data, X. karsticola NBIMCC 9097 was grouped along with other X. karsticola isolates, although the DNA sequence of the novel X. karsticola was rather distantly related to the other X. karsticola sequence data. The results were supported by the bootstrap analysis (100%) and indicated the different origin of the examined X. karsticola NBIMCC 9097.