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An amino acid substitution in HCV core antigen limits its use as a reliable measure of HCV infection compared with HCV RNA
Hepatitis C virus (HCV) is a viral pathogen that causes chronic hepatitis, which can lead to cirrhosis and hepatocellular carcinoma. Detection of HCV RNA is the standard method used to diagnose the disease and monitor antiviral treatment. A quantification assay for the HCV core antigen (HCVcAg) has...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310030/ https://www.ncbi.nlm.nih.gov/pubmed/37384719 http://dx.doi.org/10.1371/journal.pone.0287694 |
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author | Hansoongnern, Payuda Pratedrat, Pornpitra Nilyanimit, Pornjarim Wasitthankasem, Rujipat Posuwan, Nawarat Wanlapakorn, Nasamon Kodchakorn, Kanchanok Kongtawelert, Prachya Pimsing, Napaporn Poovorawan, Yong |
author_facet | Hansoongnern, Payuda Pratedrat, Pornpitra Nilyanimit, Pornjarim Wasitthankasem, Rujipat Posuwan, Nawarat Wanlapakorn, Nasamon Kodchakorn, Kanchanok Kongtawelert, Prachya Pimsing, Napaporn Poovorawan, Yong |
author_sort | Hansoongnern, Payuda |
collection | PubMed |
description | Hepatitis C virus (HCV) is a viral pathogen that causes chronic hepatitis, which can lead to cirrhosis and hepatocellular carcinoma. Detection of HCV RNA is the standard method used to diagnose the disease and monitor antiviral treatment. A quantification assay for the HCV core antigen (HCVcAg) has been proposed as a simplified alternative to the HCV RNA test for predicting active HCV infection, with the aim of achieving the global goal of eliminating hepatitis. The objective of this study was to determine the correlation between HCV RNA and HCVcAg, as well as the impact of amino acid sequence heterogeneity on HCVcAg quantification. Our findings demonstrated a strong positive correlation between HCV RNA and HCVcAg across all HCV genotypes (1a, 1b, 3a, and 6), with correlation coefficients ranging from 0.88 to 0.96 (p < 0.001). However, in some cases, samples with genotypes 3a and 6 exhibited lower HCVcAg levels than expected based on the corresponding HCV RNA values. Upon the core amino acid sequence alignment, it was observed that samples exhibiting low core antigen levels had an amino acid substitution at position 49, where threonine was replaced by either alanine or valine. Core mutation at this position may correlate with one of the epitope regions recognized by anti-HCV monoclonal antibodies. The present findings suggest that the utilization of HCVcAg as a standalone marker for HCV RNA might not provide adequate sensitivity for the detection of HCV infection, especially in cases where there are variations in the amino acid sequence of the core region and a low viral load of HCV RNA. |
format | Online Article Text |
id | pubmed-10310030 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-103100302023-06-30 An amino acid substitution in HCV core antigen limits its use as a reliable measure of HCV infection compared with HCV RNA Hansoongnern, Payuda Pratedrat, Pornpitra Nilyanimit, Pornjarim Wasitthankasem, Rujipat Posuwan, Nawarat Wanlapakorn, Nasamon Kodchakorn, Kanchanok Kongtawelert, Prachya Pimsing, Napaporn Poovorawan, Yong PLoS One Research Article Hepatitis C virus (HCV) is a viral pathogen that causes chronic hepatitis, which can lead to cirrhosis and hepatocellular carcinoma. Detection of HCV RNA is the standard method used to diagnose the disease and monitor antiviral treatment. A quantification assay for the HCV core antigen (HCVcAg) has been proposed as a simplified alternative to the HCV RNA test for predicting active HCV infection, with the aim of achieving the global goal of eliminating hepatitis. The objective of this study was to determine the correlation between HCV RNA and HCVcAg, as well as the impact of amino acid sequence heterogeneity on HCVcAg quantification. Our findings demonstrated a strong positive correlation between HCV RNA and HCVcAg across all HCV genotypes (1a, 1b, 3a, and 6), with correlation coefficients ranging from 0.88 to 0.96 (p < 0.001). However, in some cases, samples with genotypes 3a and 6 exhibited lower HCVcAg levels than expected based on the corresponding HCV RNA values. Upon the core amino acid sequence alignment, it was observed that samples exhibiting low core antigen levels had an amino acid substitution at position 49, where threonine was replaced by either alanine or valine. Core mutation at this position may correlate with one of the epitope regions recognized by anti-HCV monoclonal antibodies. The present findings suggest that the utilization of HCVcAg as a standalone marker for HCV RNA might not provide adequate sensitivity for the detection of HCV infection, especially in cases where there are variations in the amino acid sequence of the core region and a low viral load of HCV RNA. Public Library of Science 2023-06-29 /pmc/articles/PMC10310030/ /pubmed/37384719 http://dx.doi.org/10.1371/journal.pone.0287694 Text en © 2023 Hansoongnern et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hansoongnern, Payuda Pratedrat, Pornpitra Nilyanimit, Pornjarim Wasitthankasem, Rujipat Posuwan, Nawarat Wanlapakorn, Nasamon Kodchakorn, Kanchanok Kongtawelert, Prachya Pimsing, Napaporn Poovorawan, Yong An amino acid substitution in HCV core antigen limits its use as a reliable measure of HCV infection compared with HCV RNA |
title | An amino acid substitution in HCV core antigen limits its use as a reliable measure of HCV infection compared with HCV RNA |
title_full | An amino acid substitution in HCV core antigen limits its use as a reliable measure of HCV infection compared with HCV RNA |
title_fullStr | An amino acid substitution in HCV core antigen limits its use as a reliable measure of HCV infection compared with HCV RNA |
title_full_unstemmed | An amino acid substitution in HCV core antigen limits its use as a reliable measure of HCV infection compared with HCV RNA |
title_short | An amino acid substitution in HCV core antigen limits its use as a reliable measure of HCV infection compared with HCV RNA |
title_sort | amino acid substitution in hcv core antigen limits its use as a reliable measure of hcv infection compared with hcv rna |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310030/ https://www.ncbi.nlm.nih.gov/pubmed/37384719 http://dx.doi.org/10.1371/journal.pone.0287694 |
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