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Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries

Caprine papillomaviruses (ChPVs, Capra hircus papillomaviruses) were detected and quantified for the first time using droplet digital polymerase chain reaction (ddPCR) in blood samples of 374 clinically healthy goats from farms located in Italy, Romania, and Serbia. Overall, ddPCR revealed ChPV DNA...

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Autores principales: Cutarelli, Anna, De Falco, Francesca, Cuccaro, Bianca, Milićević, Vesna, Kureljušić, Branislav, Bojkovski, Jovan, Cerino, Pellegrino, Perillo, Antonella, Marica, Raluca, Catoi, Cornel, Roperto, Sante
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310300/
https://www.ncbi.nlm.nih.gov/pubmed/37396991
http://dx.doi.org/10.3389/fvets.2023.1213150
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author Cutarelli, Anna
De Falco, Francesca
Cuccaro, Bianca
Milićević, Vesna
Kureljušić, Branislav
Bojkovski, Jovan
Cerino, Pellegrino
Perillo, Antonella
Marica, Raluca
Catoi, Cornel
Roperto, Sante
author_facet Cutarelli, Anna
De Falco, Francesca
Cuccaro, Bianca
Milićević, Vesna
Kureljušić, Branislav
Bojkovski, Jovan
Cerino, Pellegrino
Perillo, Antonella
Marica, Raluca
Catoi, Cornel
Roperto, Sante
author_sort Cutarelli, Anna
collection PubMed
description Caprine papillomaviruses (ChPVs, Capra hircus papillomaviruses) were detected and quantified for the first time using droplet digital polymerase chain reaction (ddPCR) in blood samples of 374 clinically healthy goats from farms located in Italy, Romania, and Serbia. Overall, ddPCR revealed ChPV DNA in 78 of the 374 examined samples, indicating that ~21% of the goats harbored circulating papillomavirus DNA. In particular, in Italian goat farms, ChPV genotypes were detected and quantified in 58 of 157 blood samples (~37%), 11 of 117 samples from Serbian farms (~9.4%), and 9 of 100 from Romanian blood samples (9%). Blood samples from Italian goat farms showed a high prevalence of ChPV1, which was detected in 45 samples (28.6%). The ChPV2 genotype was detected in 13 samples (~8.3%). Therefore, significant differences in prevalence and genotype distributions were observed. On Serbian and Romanian farms, no significant differences were observed in the genotype prevalence of ChPVs. Molecular findings are consistent with ChPV prevalence, characterized by a territorial distribution similar to that of papillomaviruses in other mammalian species. Furthermore, this study showed that ddPCR is a very sensitive and accurate assay for ChPV detection and quantification. The ddPCR may be the molecular diagnostic tool of choice, ultimately providing useful insights into the molecular epidemiology and field surveillance of ChPV.
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spelling pubmed-103103002023-06-30 Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries Cutarelli, Anna De Falco, Francesca Cuccaro, Bianca Milićević, Vesna Kureljušić, Branislav Bojkovski, Jovan Cerino, Pellegrino Perillo, Antonella Marica, Raluca Catoi, Cornel Roperto, Sante Front Vet Sci Veterinary Science Caprine papillomaviruses (ChPVs, Capra hircus papillomaviruses) were detected and quantified for the first time using droplet digital polymerase chain reaction (ddPCR) in blood samples of 374 clinically healthy goats from farms located in Italy, Romania, and Serbia. Overall, ddPCR revealed ChPV DNA in 78 of the 374 examined samples, indicating that ~21% of the goats harbored circulating papillomavirus DNA. In particular, in Italian goat farms, ChPV genotypes were detected and quantified in 58 of 157 blood samples (~37%), 11 of 117 samples from Serbian farms (~9.4%), and 9 of 100 from Romanian blood samples (9%). Blood samples from Italian goat farms showed a high prevalence of ChPV1, which was detected in 45 samples (28.6%). The ChPV2 genotype was detected in 13 samples (~8.3%). Therefore, significant differences in prevalence and genotype distributions were observed. On Serbian and Romanian farms, no significant differences were observed in the genotype prevalence of ChPVs. Molecular findings are consistent with ChPV prevalence, characterized by a territorial distribution similar to that of papillomaviruses in other mammalian species. Furthermore, this study showed that ddPCR is a very sensitive and accurate assay for ChPV detection and quantification. The ddPCR may be the molecular diagnostic tool of choice, ultimately providing useful insights into the molecular epidemiology and field surveillance of ChPV. Frontiers Media S.A. 2023-06-15 /pmc/articles/PMC10310300/ /pubmed/37396991 http://dx.doi.org/10.3389/fvets.2023.1213150 Text en Copyright © 2023 Cutarelli, De Falco, Cuccaro, Milićević, Kureljušić, Bojkovski, Cerino, Perillo, Marica, Catoi and Roperto. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Cutarelli, Anna
De Falco, Francesca
Cuccaro, Bianca
Milićević, Vesna
Kureljušić, Branislav
Bojkovski, Jovan
Cerino, Pellegrino
Perillo, Antonella
Marica, Raluca
Catoi, Cornel
Roperto, Sante
Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries
title Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries
title_full Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries
title_fullStr Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries
title_full_unstemmed Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries
title_short Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries
title_sort prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three european countries
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310300/
https://www.ncbi.nlm.nih.gov/pubmed/37396991
http://dx.doi.org/10.3389/fvets.2023.1213150
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