Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain

When a monkeypox virus outbreak began in several parts of the world in May 2022, timely and accurate diagnosis became mandatory. In our laboratory, a real-time quantitative PCR was designed and evaluated in several patient samples and compared with isolation results. Genomic viral load was related t...

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Autores principales: Álvarez Argüelles, Marta E., Martínez, Zulema Pérez, Alba, Susana Rojo, González Alba, Jose María, Fernandez-Verdugo, Ana María, González, Isabel Costales, Rodríguez, Garbriel Martín, Riveiro, Jose Antonio Boga, Martins, Mario Margolles, García, Santiago Melón
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2023
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310368/
https://www.ncbi.nlm.nih.gov/pubmed/37347839
http://dx.doi.org/10.3201/eid2907.221229
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author Álvarez Argüelles, Marta E.
Martínez, Zulema Pérez
Alba, Susana Rojo
González Alba, Jose María
Fernandez-Verdugo, Ana María
González, Isabel Costales
Rodríguez, Garbriel Martín
Riveiro, Jose Antonio Boga
Martins, Mario Margolles
García, Santiago Melón
author_facet Álvarez Argüelles, Marta E.
Martínez, Zulema Pérez
Alba, Susana Rojo
González Alba, Jose María
Fernandez-Verdugo, Ana María
González, Isabel Costales
Rodríguez, Garbriel Martín
Riveiro, Jose Antonio Boga
Martins, Mario Margolles
García, Santiago Melón
author_sort Álvarez Argüelles, Marta E.
collection PubMed
description When a monkeypox virus outbreak began in several parts of the world in May 2022, timely and accurate diagnosis became mandatory. In our laboratory, a real-time quantitative PCR was designed and evaluated in several patient samples and compared with isolation results. Genomic viral load was related to virus viability.
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spelling pubmed-103103682023-07-01 Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain Álvarez Argüelles, Marta E. Martínez, Zulema Pérez Alba, Susana Rojo González Alba, Jose María Fernandez-Verdugo, Ana María González, Isabel Costales Rodríguez, Garbriel Martín Riveiro, Jose Antonio Boga Martins, Mario Margolles García, Santiago Melón Emerg Infect Dis Dispatch When a monkeypox virus outbreak began in several parts of the world in May 2022, timely and accurate diagnosis became mandatory. In our laboratory, a real-time quantitative PCR was designed and evaluated in several patient samples and compared with isolation results. Genomic viral load was related to virus viability. Centers for Disease Control and Prevention 2023-07 /pmc/articles/PMC10310368/ /pubmed/37347839 http://dx.doi.org/10.3201/eid2907.221229 Text en https://creativecommons.org/licenses/by/4.0/Emerging Infectious Diseases is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Álvarez Argüelles, Marta E.
Martínez, Zulema Pérez
Alba, Susana Rojo
González Alba, Jose María
Fernandez-Verdugo, Ana María
González, Isabel Costales
Rodríguez, Garbriel Martín
Riveiro, Jose Antonio Boga
Martins, Mario Margolles
García, Santiago Melón
Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain
title Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain
title_full Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain
title_fullStr Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain
title_full_unstemmed Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain
title_short Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain
title_sort detecting, quantifying, and isolating monkeypox virus in suspected cases, spain
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310368/
https://www.ncbi.nlm.nih.gov/pubmed/37347839
http://dx.doi.org/10.3201/eid2907.221229
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