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Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites

INTRODUCTION: A safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. Its cell-free supernatant (LAB-CFS), which is rich in bioactive compounds, is what makes LAB antibacterial work. METHODS: This study focused on the changes in...

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Autores principales: Mao, Yanni, Wang, Yuxia, Luo, Xiaofeng, Chen, Xiaohui, Wang, Guiqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310794/
https://www.ncbi.nlm.nih.gov/pubmed/37397004
http://dx.doi.org/10.3389/fvets.2023.1184989
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author Mao, Yanni
Wang, Yuxia
Luo, Xiaofeng
Chen, Xiaohui
Wang, Guiqin
author_facet Mao, Yanni
Wang, Yuxia
Luo, Xiaofeng
Chen, Xiaohui
Wang, Guiqin
author_sort Mao, Yanni
collection PubMed
description INTRODUCTION: A safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. Its cell-free supernatant (LAB-CFS), which is rich in bioactive compounds, is what makes LAB antibacterial work. METHODS: This study focused on the changes in biofilm activity and related metabolic pathways of S. aureus treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS). RESULTS: The findings demonstrated that the LAB-CFS treatment considerably slowed Staphylococcus aureus (S. aureus) growth and prevented it from forming biofilms. Additionally, it inhibits the physiological traits of the S. aureus biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. The metabolites of S. aureus biofilm treated with LAB-CFS were greater in the LAB-bf-CFS than they were in the LAB-pk-CFS, according to metabolomics studies. Important metabolic pathways such amino acids and carbohydrates metabolism were among the most noticeably altered metabolic pathways. DISCUSSION: These findings show that LAB-CFS has a strong potential to combat S. aureus infections.
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spelling pubmed-103107942023-07-01 Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites Mao, Yanni Wang, Yuxia Luo, Xiaofeng Chen, Xiaohui Wang, Guiqin Front Vet Sci Veterinary Science INTRODUCTION: A safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. Its cell-free supernatant (LAB-CFS), which is rich in bioactive compounds, is what makes LAB antibacterial work. METHODS: This study focused on the changes in biofilm activity and related metabolic pathways of S. aureus treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS). RESULTS: The findings demonstrated that the LAB-CFS treatment considerably slowed Staphylococcus aureus (S. aureus) growth and prevented it from forming biofilms. Additionally, it inhibits the physiological traits of the S. aureus biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. The metabolites of S. aureus biofilm treated with LAB-CFS were greater in the LAB-bf-CFS than they were in the LAB-pk-CFS, according to metabolomics studies. Important metabolic pathways such amino acids and carbohydrates metabolism were among the most noticeably altered metabolic pathways. DISCUSSION: These findings show that LAB-CFS has a strong potential to combat S. aureus infections. Frontiers Media S.A. 2023-06-15 /pmc/articles/PMC10310794/ /pubmed/37397004 http://dx.doi.org/10.3389/fvets.2023.1184989 Text en Copyright © 2023 Mao, Wang, Luo, Chen and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Mao, Yanni
Wang, Yuxia
Luo, Xiaofeng
Chen, Xiaohui
Wang, Guiqin
Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites
title Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites
title_full Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites
title_fullStr Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites
title_full_unstemmed Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites
title_short Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites
title_sort impact of cell-free supernatant of lactic acid bacteria on staphylococcus aureus biofilm and its metabolites
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310794/
https://www.ncbi.nlm.nih.gov/pubmed/37397004
http://dx.doi.org/10.3389/fvets.2023.1184989
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