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Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites
INTRODUCTION: A safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. Its cell-free supernatant (LAB-CFS), which is rich in bioactive compounds, is what makes LAB antibacterial work. METHODS: This study focused on the changes in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310794/ https://www.ncbi.nlm.nih.gov/pubmed/37397004 http://dx.doi.org/10.3389/fvets.2023.1184989 |
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author | Mao, Yanni Wang, Yuxia Luo, Xiaofeng Chen, Xiaohui Wang, Guiqin |
author_facet | Mao, Yanni Wang, Yuxia Luo, Xiaofeng Chen, Xiaohui Wang, Guiqin |
author_sort | Mao, Yanni |
collection | PubMed |
description | INTRODUCTION: A safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. Its cell-free supernatant (LAB-CFS), which is rich in bioactive compounds, is what makes LAB antibacterial work. METHODS: This study focused on the changes in biofilm activity and related metabolic pathways of S. aureus treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS). RESULTS: The findings demonstrated that the LAB-CFS treatment considerably slowed Staphylococcus aureus (S. aureus) growth and prevented it from forming biofilms. Additionally, it inhibits the physiological traits of the S. aureus biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. The metabolites of S. aureus biofilm treated with LAB-CFS were greater in the LAB-bf-CFS than they were in the LAB-pk-CFS, according to metabolomics studies. Important metabolic pathways such amino acids and carbohydrates metabolism were among the most noticeably altered metabolic pathways. DISCUSSION: These findings show that LAB-CFS has a strong potential to combat S. aureus infections. |
format | Online Article Text |
id | pubmed-10310794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103107942023-07-01 Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites Mao, Yanni Wang, Yuxia Luo, Xiaofeng Chen, Xiaohui Wang, Guiqin Front Vet Sci Veterinary Science INTRODUCTION: A safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. Its cell-free supernatant (LAB-CFS), which is rich in bioactive compounds, is what makes LAB antibacterial work. METHODS: This study focused on the changes in biofilm activity and related metabolic pathways of S. aureus treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS). RESULTS: The findings demonstrated that the LAB-CFS treatment considerably slowed Staphylococcus aureus (S. aureus) growth and prevented it from forming biofilms. Additionally, it inhibits the physiological traits of the S. aureus biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. The metabolites of S. aureus biofilm treated with LAB-CFS were greater in the LAB-bf-CFS than they were in the LAB-pk-CFS, according to metabolomics studies. Important metabolic pathways such amino acids and carbohydrates metabolism were among the most noticeably altered metabolic pathways. DISCUSSION: These findings show that LAB-CFS has a strong potential to combat S. aureus infections. Frontiers Media S.A. 2023-06-15 /pmc/articles/PMC10310794/ /pubmed/37397004 http://dx.doi.org/10.3389/fvets.2023.1184989 Text en Copyright © 2023 Mao, Wang, Luo, Chen and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Mao, Yanni Wang, Yuxia Luo, Xiaofeng Chen, Xiaohui Wang, Guiqin Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites |
title | Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites |
title_full | Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites |
title_fullStr | Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites |
title_full_unstemmed | Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites |
title_short | Impact of cell-free supernatant of lactic acid bacteria on Staphylococcus aureus biofilm and its metabolites |
title_sort | impact of cell-free supernatant of lactic acid bacteria on staphylococcus aureus biofilm and its metabolites |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310794/ https://www.ncbi.nlm.nih.gov/pubmed/37397004 http://dx.doi.org/10.3389/fvets.2023.1184989 |
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