Cargando…
Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis
OBJECTIVE: To determine the effects of sperm deoxyribonucleic acid (DNA) fragmentation at the time of fertilization on in vitro fertilization (IVF) outcomes and genetic diagnosis using next generation sequencing. DESIGN: Prospective double-blinded study. SETTING: Private Clinic. PATIENTS: Couples (n...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310935/ https://www.ncbi.nlm.nih.gov/pubmed/37398612 http://dx.doi.org/10.1016/j.xfre.2023.04.006 |
_version_ | 1785066638708572160 |
---|---|
author | Broussard, Alicia L. Leader, Benjamin Tirado, Edna Russell, Helena Beydoun, Hind Colver, Robert Reuter, Laura Bopp, Bradford Will, Matthew Anspach Will, Erica Adaniya, Glen |
author_facet | Broussard, Alicia L. Leader, Benjamin Tirado, Edna Russell, Helena Beydoun, Hind Colver, Robert Reuter, Laura Bopp, Bradford Will, Matthew Anspach Will, Erica Adaniya, Glen |
author_sort | Broussard, Alicia L. |
collection | PubMed |
description | OBJECTIVE: To determine the effects of sperm deoxyribonucleic acid (DNA) fragmentation at the time of fertilization on in vitro fertilization (IVF) outcomes and genetic diagnosis using next generation sequencing. DESIGN: Prospective double-blinded study. SETTING: Private Clinic. PATIENTS: Couples (n = 150). INTERVENTION: In vitro fertilization with preimplantation genetic testing for aneuploidy and sperm DNA fragmentation assay, as in sperm chromatin structure assay the day of retrieval. MAIN OUTCOME MEASURES: Laboratory outcomes are listed in the results section. Statistical analysis was performed using JMP, XYLSTAT, and STATA version 15. RESULTS: The sperm DNA fragmentation index (DFI) in the neat ejaculate did not predict fertilization rate, quality, blastulation, or genetic diagnosis. No statistically significant results were obtained comparing <15% with >15%, <20% with >20%, <30% with >30% except for DFI. No statistically significant differences in oocyte source age or male age were observed. No statistically significant differences comparing <15% with >15%, <20% with >20%, <30% with >30% DFI at the time of standard IVF or intracytoplasmic sperm injection (ICSI) were observed for % euploid, aneuploid, mosaic, blastulation, biopsied, or D5/total biopsied. The DFI of >15% had more good quality D3 embryos than the <15% group, as did the >20% group compared with the <20% group. The ICSI fertilization was significantly higher in all 3 lower percentage groups compared with the higher counterpart. Standard IVF had significantly more blastocysts/fertilized suitable for biopsy and more D5/total number biopsied than ICSI embryos despite no difference in DFI. CONCLUSIONS: The DFI at fertilization is correlated with decreased fertilization for ICSI and IVF. |
format | Online Article Text |
id | pubmed-10310935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-103109352023-07-01 Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis Broussard, Alicia L. Leader, Benjamin Tirado, Edna Russell, Helena Beydoun, Hind Colver, Robert Reuter, Laura Bopp, Bradford Will, Matthew Anspach Will, Erica Adaniya, Glen F S Rep Original Article OBJECTIVE: To determine the effects of sperm deoxyribonucleic acid (DNA) fragmentation at the time of fertilization on in vitro fertilization (IVF) outcomes and genetic diagnosis using next generation sequencing. DESIGN: Prospective double-blinded study. SETTING: Private Clinic. PATIENTS: Couples (n = 150). INTERVENTION: In vitro fertilization with preimplantation genetic testing for aneuploidy and sperm DNA fragmentation assay, as in sperm chromatin structure assay the day of retrieval. MAIN OUTCOME MEASURES: Laboratory outcomes are listed in the results section. Statistical analysis was performed using JMP, XYLSTAT, and STATA version 15. RESULTS: The sperm DNA fragmentation index (DFI) in the neat ejaculate did not predict fertilization rate, quality, blastulation, or genetic diagnosis. No statistically significant results were obtained comparing <15% with >15%, <20% with >20%, <30% with >30% except for DFI. No statistically significant differences in oocyte source age or male age were observed. No statistically significant differences comparing <15% with >15%, <20% with >20%, <30% with >30% DFI at the time of standard IVF or intracytoplasmic sperm injection (ICSI) were observed for % euploid, aneuploid, mosaic, blastulation, biopsied, or D5/total biopsied. The DFI of >15% had more good quality D3 embryos than the <15% group, as did the >20% group compared with the <20% group. The ICSI fertilization was significantly higher in all 3 lower percentage groups compared with the higher counterpart. Standard IVF had significantly more blastocysts/fertilized suitable for biopsy and more D5/total number biopsied than ICSI embryos despite no difference in DFI. CONCLUSIONS: The DFI at fertilization is correlated with decreased fertilization for ICSI and IVF. Elsevier 2023-04-25 /pmc/articles/PMC10310935/ /pubmed/37398612 http://dx.doi.org/10.1016/j.xfre.2023.04.006 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Broussard, Alicia L. Leader, Benjamin Tirado, Edna Russell, Helena Beydoun, Hind Colver, Robert Reuter, Laura Bopp, Bradford Will, Matthew Anspach Will, Erica Adaniya, Glen Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
title | Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
title_full | Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
title_fullStr | Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
title_full_unstemmed | Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
title_short | Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
title_sort | sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310935/ https://www.ncbi.nlm.nih.gov/pubmed/37398612 http://dx.doi.org/10.1016/j.xfre.2023.04.006 |
work_keys_str_mv | AT broussardalicial spermdeoxyribonucleicacidfragmentationindexatthetimeofintracytoplasmicsperminjectionandstandardinvitrofertilizationiscorrelatedwithlowerfertilizationbutnotwithblastocystgeneticdiagnosis AT leaderbenjamin spermdeoxyribonucleicacidfragmentationindexatthetimeofintracytoplasmicsperminjectionandstandardinvitrofertilizationiscorrelatedwithlowerfertilizationbutnotwithblastocystgeneticdiagnosis AT tiradoedna spermdeoxyribonucleicacidfragmentationindexatthetimeofintracytoplasmicsperminjectionandstandardinvitrofertilizationiscorrelatedwithlowerfertilizationbutnotwithblastocystgeneticdiagnosis AT russellhelena spermdeoxyribonucleicacidfragmentationindexatthetimeofintracytoplasmicsperminjectionandstandardinvitrofertilizationiscorrelatedwithlowerfertilizationbutnotwithblastocystgeneticdiagnosis AT beydounhind spermdeoxyribonucleicacidfragmentationindexatthetimeofintracytoplasmicsperminjectionandstandardinvitrofertilizationiscorrelatedwithlowerfertilizationbutnotwithblastocystgeneticdiagnosis AT colverrobert spermdeoxyribonucleicacidfragmentationindexatthetimeofintracytoplasmicsperminjectionandstandardinvitrofertilizationiscorrelatedwithlowerfertilizationbutnotwithblastocystgeneticdiagnosis AT reuterlaura spermdeoxyribonucleicacidfragmentationindexatthetimeofintracytoplasmicsperminjectionandstandardinvitrofertilizationiscorrelatedwithlowerfertilizationbutnotwithblastocystgeneticdiagnosis AT boppbradford spermdeoxyribonucleicacidfragmentationindexatthetimeofintracytoplasmicsperminjectionandstandardinvitrofertilizationiscorrelatedwithlowerfertilizationbutnotwithblastocystgeneticdiagnosis AT willmatthew spermdeoxyribonucleicacidfragmentationindexatthetimeofintracytoplasmicsperminjectionandstandardinvitrofertilizationiscorrelatedwithlowerfertilizationbutnotwithblastocystgeneticdiagnosis AT anspachwillerica spermdeoxyribonucleicacidfragmentationindexatthetimeofintracytoplasmicsperminjectionandstandardinvitrofertilizationiscorrelatedwithlowerfertilizationbutnotwithblastocystgeneticdiagnosis AT adaniyaglen spermdeoxyribonucleicacidfragmentationindexatthetimeofintracytoplasmicsperminjectionandstandardinvitrofertilizationiscorrelatedwithlowerfertilizationbutnotwithblastocystgeneticdiagnosis |