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Novel leaderless bacteriocin geobacillin 6 from thermophilic bacterium Parageobacillus thermoglucosidasius

Bacterial resistance to conventional antibiotics has urged us to develop alternative strategies against bacterial pathogens. Moreover, a demand for food products containing no chemical preservatives has led us to search for new alternative technologies for food preservation. Bacteriocins – ribosomal...

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Autores principales: Koniuchovaitė, Ana, Petkevičiūtė, Akvilė, Bernotaitė, Emilija, Gricajeva, Alisa, Gegeckas, Audrius, Kalėdienė, Lilija, Kaunietis, Arnoldas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10311245/
https://www.ncbi.nlm.nih.gov/pubmed/37396380
http://dx.doi.org/10.3389/fmicb.2023.1207367
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author Koniuchovaitė, Ana
Petkevičiūtė, Akvilė
Bernotaitė, Emilija
Gricajeva, Alisa
Gegeckas, Audrius
Kalėdienė, Lilija
Kaunietis, Arnoldas
author_facet Koniuchovaitė, Ana
Petkevičiūtė, Akvilė
Bernotaitė, Emilija
Gricajeva, Alisa
Gegeckas, Audrius
Kalėdienė, Lilija
Kaunietis, Arnoldas
author_sort Koniuchovaitė, Ana
collection PubMed
description Bacterial resistance to conventional antibiotics has urged us to develop alternative strategies against bacterial pathogens. Moreover, a demand for food products containing no chemical preservatives has led us to search for new alternative technologies for food preservation. Bacteriocins – ribosomally synthesized antimicrobial peptides – have been proposed as a new alternative to conventional antibiotics or chemicals for food preservation. This study describes biosynthesis and characterization of a novel leaderless bacteriocin, geobacillin 6, which was identified in the thermophilic bacterium Parageobacillus thermoglucosidasius. Its amino acid sequence shows low similarity to other bacteriocins and it is the first leaderless-type bacteriocin identified in thermophilic bacteria. Based on structure assessment, the bacteriocin forms a multi-helix bundle. Geobacillin 6 exhibits a relatively narrow antimicrobial spectrum, it is active in the μM range and against Gram-positive bacteria, mostly thermophilic species closely related to the producer strain. Bacteriocin demonstrates stability over pH 3–11 and is highly thermostable, retaining 100% of its activity after incubation at 95°C for 6 h. Geobacillin 6 has potential in the food industry and biotechnological processes where contamination with thermophilic bacteria is undesirable.
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spelling pubmed-103112452023-07-01 Novel leaderless bacteriocin geobacillin 6 from thermophilic bacterium Parageobacillus thermoglucosidasius Koniuchovaitė, Ana Petkevičiūtė, Akvilė Bernotaitė, Emilija Gricajeva, Alisa Gegeckas, Audrius Kalėdienė, Lilija Kaunietis, Arnoldas Front Microbiol Microbiology Bacterial resistance to conventional antibiotics has urged us to develop alternative strategies against bacterial pathogens. Moreover, a demand for food products containing no chemical preservatives has led us to search for new alternative technologies for food preservation. Bacteriocins – ribosomally synthesized antimicrobial peptides – have been proposed as a new alternative to conventional antibiotics or chemicals for food preservation. This study describes biosynthesis and characterization of a novel leaderless bacteriocin, geobacillin 6, which was identified in the thermophilic bacterium Parageobacillus thermoglucosidasius. Its amino acid sequence shows low similarity to other bacteriocins and it is the first leaderless-type bacteriocin identified in thermophilic bacteria. Based on structure assessment, the bacteriocin forms a multi-helix bundle. Geobacillin 6 exhibits a relatively narrow antimicrobial spectrum, it is active in the μM range and against Gram-positive bacteria, mostly thermophilic species closely related to the producer strain. Bacteriocin demonstrates stability over pH 3–11 and is highly thermostable, retaining 100% of its activity after incubation at 95°C for 6 h. Geobacillin 6 has potential in the food industry and biotechnological processes where contamination with thermophilic bacteria is undesirable. Frontiers Media S.A. 2023-06-15 /pmc/articles/PMC10311245/ /pubmed/37396380 http://dx.doi.org/10.3389/fmicb.2023.1207367 Text en Copyright © 2023 Koniuchovaitė, Petkevičiūtė, Bernotaitė, Gricajeva, Gegeckas, Kalėdienė and Kaunietis. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Koniuchovaitė, Ana
Petkevičiūtė, Akvilė
Bernotaitė, Emilija
Gricajeva, Alisa
Gegeckas, Audrius
Kalėdienė, Lilija
Kaunietis, Arnoldas
Novel leaderless bacteriocin geobacillin 6 from thermophilic bacterium Parageobacillus thermoglucosidasius
title Novel leaderless bacteriocin geobacillin 6 from thermophilic bacterium Parageobacillus thermoglucosidasius
title_full Novel leaderless bacteriocin geobacillin 6 from thermophilic bacterium Parageobacillus thermoglucosidasius
title_fullStr Novel leaderless bacteriocin geobacillin 6 from thermophilic bacterium Parageobacillus thermoglucosidasius
title_full_unstemmed Novel leaderless bacteriocin geobacillin 6 from thermophilic bacterium Parageobacillus thermoglucosidasius
title_short Novel leaderless bacteriocin geobacillin 6 from thermophilic bacterium Parageobacillus thermoglucosidasius
title_sort novel leaderless bacteriocin geobacillin 6 from thermophilic bacterium parageobacillus thermoglucosidasius
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10311245/
https://www.ncbi.nlm.nih.gov/pubmed/37396380
http://dx.doi.org/10.3389/fmicb.2023.1207367
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