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Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2
Herpes simplex virus type 1 (HSV-1) causes lifelong infections worldwide, and currently there is no efficient cure or vaccine. HSV-1-derived tools, such as neuronal circuit tracers and oncolytic viruses, have been used extensively; however, further genetic engineering of HSV-1 is hindered by its com...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wuhan Institute of Virology, Chinese Academy of Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10311267/ https://www.ncbi.nlm.nih.gov/pubmed/36940800 http://dx.doi.org/10.1016/j.virs.2023.03.005 |
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author | Xiao, Han Hu, Hengrui Guo, Yijia Li, Jiang Wen, Le Zeng, Wen-Bo Wang, Manli Luo, Min-Hua Hu, Zhihong |
author_facet | Xiao, Han Hu, Hengrui Guo, Yijia Li, Jiang Wen, Le Zeng, Wen-Bo Wang, Manli Luo, Min-Hua Hu, Zhihong |
author_sort | Xiao, Han |
collection | PubMed |
description | Herpes simplex virus type 1 (HSV-1) causes lifelong infections worldwide, and currently there is no efficient cure or vaccine. HSV-1-derived tools, such as neuronal circuit tracers and oncolytic viruses, have been used extensively; however, further genetic engineering of HSV-1 is hindered by its complex genome structure. In the present study, we designed and constructed a synthetic platform for HSV-1 based on H129-G4. The complete genome was constructed from 10 fragments through 3 rounds of synthesis using transformation-associated recombination (TAR) in yeast, and was named H129-Syn-G2. The H129-Syn-G2 genome contained two copies of the gfp gene and was transfected into cells to rescue the virus. According to growth curve assay and electron microscopy results, the synthetic viruses exhibited more optimized growth properties and similar morphogenesis compared to the parental virus. This synthetic platform will facilitate further manipulation of the HSV-1 genome for the development of neuronal circuit tracers, oncolytic viruses, and vaccines. |
format | Online Article Text |
id | pubmed-10311267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Wuhan Institute of Virology, Chinese Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-103112672023-07-01 Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2 Xiao, Han Hu, Hengrui Guo, Yijia Li, Jiang Wen, Le Zeng, Wen-Bo Wang, Manli Luo, Min-Hua Hu, Zhihong Virol Sin Research Article Herpes simplex virus type 1 (HSV-1) causes lifelong infections worldwide, and currently there is no efficient cure or vaccine. HSV-1-derived tools, such as neuronal circuit tracers and oncolytic viruses, have been used extensively; however, further genetic engineering of HSV-1 is hindered by its complex genome structure. In the present study, we designed and constructed a synthetic platform for HSV-1 based on H129-G4. The complete genome was constructed from 10 fragments through 3 rounds of synthesis using transformation-associated recombination (TAR) in yeast, and was named H129-Syn-G2. The H129-Syn-G2 genome contained two copies of the gfp gene and was transfected into cells to rescue the virus. According to growth curve assay and electron microscopy results, the synthetic viruses exhibited more optimized growth properties and similar morphogenesis compared to the parental virus. This synthetic platform will facilitate further manipulation of the HSV-1 genome for the development of neuronal circuit tracers, oncolytic viruses, and vaccines. Wuhan Institute of Virology, Chinese Academy of Sciences 2023-03-20 /pmc/articles/PMC10311267/ /pubmed/36940800 http://dx.doi.org/10.1016/j.virs.2023.03.005 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Xiao, Han Hu, Hengrui Guo, Yijia Li, Jiang Wen, Le Zeng, Wen-Bo Wang, Manli Luo, Min-Hua Hu, Zhihong Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2 |
title | Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2 |
title_full | Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2 |
title_fullStr | Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2 |
title_full_unstemmed | Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2 |
title_short | Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2 |
title_sort | construction and characterization of a synthesized herpes simplex virus h129-syn-g2 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10311267/ https://www.ncbi.nlm.nih.gov/pubmed/36940800 http://dx.doi.org/10.1016/j.virs.2023.03.005 |
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