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Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein

Human immunodeficiency virus-1 (HIV-1) encodes simply 15 proteins and thus depends on multiple host cellular factors for virus reproduction. Spastin, a microtubule severing protein, is an identified HIV-1 dependency factor, but the mechanism regulating HIV-1 is unclear. Here, the study showed that k...

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Autores principales: Shen, Wenyuan, Liu, Chang, Hu, Yue, Ding, Qian, Feng, Jiabin, Liu, Zhou, Kong, Xiaohong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10311276/
https://www.ncbi.nlm.nih.gov/pubmed/37172824
http://dx.doi.org/10.1016/j.virs.2023.05.006
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author Shen, Wenyuan
Liu, Chang
Hu, Yue
Ding, Qian
Feng, Jiabin
Liu, Zhou
Kong, Xiaohong
author_facet Shen, Wenyuan
Liu, Chang
Hu, Yue
Ding, Qian
Feng, Jiabin
Liu, Zhou
Kong, Xiaohong
author_sort Shen, Wenyuan
collection PubMed
description Human immunodeficiency virus-1 (HIV-1) encodes simply 15 proteins and thus depends on multiple host cellular factors for virus reproduction. Spastin, a microtubule severing protein, is an identified HIV-1 dependency factor, but the mechanism regulating HIV-1 is unclear. Here, the study showed that knockdown of spastin inhibited the production of the intracellular HIV-1 Gag protein and new virions through enhancing Gag lysosomal degradation. Further investigation showed that increased sodium tolerance 1 (IST1), the subunit of endosomal sorting complex required for transport (ESCRT), could interact with the MIT domain of spastin to regulate the intracellular Gag production. In summary, spastin is required for HIV-1 replication, while spastin-IST1 interaction facilitates virus production by regulating HIV-1 Gag intracellular trafficking and degradation. Spastin may serve as new target for HIV-1 prophylactic and therapy.
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spelling pubmed-103112762023-07-01 Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein Shen, Wenyuan Liu, Chang Hu, Yue Ding, Qian Feng, Jiabin Liu, Zhou Kong, Xiaohong Virol Sin Research Article Human immunodeficiency virus-1 (HIV-1) encodes simply 15 proteins and thus depends on multiple host cellular factors for virus reproduction. Spastin, a microtubule severing protein, is an identified HIV-1 dependency factor, but the mechanism regulating HIV-1 is unclear. Here, the study showed that knockdown of spastin inhibited the production of the intracellular HIV-1 Gag protein and new virions through enhancing Gag lysosomal degradation. Further investigation showed that increased sodium tolerance 1 (IST1), the subunit of endosomal sorting complex required for transport (ESCRT), could interact with the MIT domain of spastin to regulate the intracellular Gag production. In summary, spastin is required for HIV-1 replication, while spastin-IST1 interaction facilitates virus production by regulating HIV-1 Gag intracellular trafficking and degradation. Spastin may serve as new target for HIV-1 prophylactic and therapy. Wuhan Institute of Virology, Chinese Academy of Sciences 2023-05-11 /pmc/articles/PMC10311276/ /pubmed/37172824 http://dx.doi.org/10.1016/j.virs.2023.05.006 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Shen, Wenyuan
Liu, Chang
Hu, Yue
Ding, Qian
Feng, Jiabin
Liu, Zhou
Kong, Xiaohong
Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein
title Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein
title_full Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein
title_fullStr Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein
title_full_unstemmed Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein
title_short Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein
title_sort spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (escrt) protein
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10311276/
https://www.ncbi.nlm.nih.gov/pubmed/37172824
http://dx.doi.org/10.1016/j.virs.2023.05.006
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