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Promising bioactive metabolites of mangrove inhabitant Streptomyces tauricus and prostate cancer PC3 cell inhibition by antimicrobial peptides

Streptomyces is a group of microbes known for antibiotic production and has contributed to more than 70% of present commercially available antibiotics. These antibiotics are important in the management, protection, and treatment of chronic illnesses. In the present study, the isolated S. tauricus st...

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Autores principales: Karthik, Yalpi, Kalyani, Manjula Ishwara, Krishnappa, Srinivasa, Ramakrishna, Krishnaveni, Sayed, Samy M., Aharthy, Ohud Muslat, Alhelaify, Seham Sater, Mushtaq, Muntazir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312093/
https://www.ncbi.nlm.nih.gov/pubmed/37396348
http://dx.doi.org/10.3389/fmicb.2023.1152985
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author Karthik, Yalpi
Kalyani, Manjula Ishwara
Krishnappa, Srinivasa
Ramakrishna, Krishnaveni
Sayed, Samy M.
Aharthy, Ohud Muslat
Alhelaify, Seham Sater
Mushtaq, Muntazir
author_facet Karthik, Yalpi
Kalyani, Manjula Ishwara
Krishnappa, Srinivasa
Ramakrishna, Krishnaveni
Sayed, Samy M.
Aharthy, Ohud Muslat
Alhelaify, Seham Sater
Mushtaq, Muntazir
author_sort Karthik, Yalpi
collection PubMed
description Streptomyces is a group of microbes known for antibiotic production and has contributed to more than 70% of present commercially available antibiotics. These antibiotics are important in the management, protection, and treatment of chronic illnesses. In the present study, the isolated S. tauricus strain from mangrove soil in Mangalore, India (GenBank accession number: MW785875) was subjected for differential cultural characterization, phenotype involving brown pigmentation, filamentous mycelia, and ash-colored spore production was observed using field emission scanning electron microscopy (FESEM) analysis revealing filamentous mycelia possessing a straight spore chain. Spores were visualized as elongated, rod-shaped, smooth surfaces with curved edges. After optimized growth conditions for S. tauricus on starch-casein agar medium, the GC/MS analysis of S. tauricus intracellular extract detected bioactive compounds reported for pharmacological applications. Analyzed using the NIST library, most of the bioactive compounds identified in intracellular extract had molecular weights of less than 1 kDa. On the PC3 cell line, the Sephadex G-10 partially purified eluted peak protein fraction demonstrated significant anticancer activity. The LCMS analysis revealed the presence of Tryprostatin B, Fumonisin B1, Microcystin LR, and Surfactin C with molecular weights below 1 kDa. This study found that small molecular weight microbial compounds are more effective in a variety of biological applications.
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spelling pubmed-103120932023-07-01 Promising bioactive metabolites of mangrove inhabitant Streptomyces tauricus and prostate cancer PC3 cell inhibition by antimicrobial peptides Karthik, Yalpi Kalyani, Manjula Ishwara Krishnappa, Srinivasa Ramakrishna, Krishnaveni Sayed, Samy M. Aharthy, Ohud Muslat Alhelaify, Seham Sater Mushtaq, Muntazir Front Microbiol Microbiology Streptomyces is a group of microbes known for antibiotic production and has contributed to more than 70% of present commercially available antibiotics. These antibiotics are important in the management, protection, and treatment of chronic illnesses. In the present study, the isolated S. tauricus strain from mangrove soil in Mangalore, India (GenBank accession number: MW785875) was subjected for differential cultural characterization, phenotype involving brown pigmentation, filamentous mycelia, and ash-colored spore production was observed using field emission scanning electron microscopy (FESEM) analysis revealing filamentous mycelia possessing a straight spore chain. Spores were visualized as elongated, rod-shaped, smooth surfaces with curved edges. After optimized growth conditions for S. tauricus on starch-casein agar medium, the GC/MS analysis of S. tauricus intracellular extract detected bioactive compounds reported for pharmacological applications. Analyzed using the NIST library, most of the bioactive compounds identified in intracellular extract had molecular weights of less than 1 kDa. On the PC3 cell line, the Sephadex G-10 partially purified eluted peak protein fraction demonstrated significant anticancer activity. The LCMS analysis revealed the presence of Tryprostatin B, Fumonisin B1, Microcystin LR, and Surfactin C with molecular weights below 1 kDa. This study found that small molecular weight microbial compounds are more effective in a variety of biological applications. Frontiers Media S.A. 2023-06-16 /pmc/articles/PMC10312093/ /pubmed/37396348 http://dx.doi.org/10.3389/fmicb.2023.1152985 Text en Copyright © 2023 Karthik, Kalyani, Krishnappa, Ramakrishna, Sayed, Aharthy, Alhelaify and Mushtaq. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Karthik, Yalpi
Kalyani, Manjula Ishwara
Krishnappa, Srinivasa
Ramakrishna, Krishnaveni
Sayed, Samy M.
Aharthy, Ohud Muslat
Alhelaify, Seham Sater
Mushtaq, Muntazir
Promising bioactive metabolites of mangrove inhabitant Streptomyces tauricus and prostate cancer PC3 cell inhibition by antimicrobial peptides
title Promising bioactive metabolites of mangrove inhabitant Streptomyces tauricus and prostate cancer PC3 cell inhibition by antimicrobial peptides
title_full Promising bioactive metabolites of mangrove inhabitant Streptomyces tauricus and prostate cancer PC3 cell inhibition by antimicrobial peptides
title_fullStr Promising bioactive metabolites of mangrove inhabitant Streptomyces tauricus and prostate cancer PC3 cell inhibition by antimicrobial peptides
title_full_unstemmed Promising bioactive metabolites of mangrove inhabitant Streptomyces tauricus and prostate cancer PC3 cell inhibition by antimicrobial peptides
title_short Promising bioactive metabolites of mangrove inhabitant Streptomyces tauricus and prostate cancer PC3 cell inhibition by antimicrobial peptides
title_sort promising bioactive metabolites of mangrove inhabitant streptomyces tauricus and prostate cancer pc3 cell inhibition by antimicrobial peptides
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312093/
https://www.ncbi.nlm.nih.gov/pubmed/37396348
http://dx.doi.org/10.3389/fmicb.2023.1152985
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