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Complete mitogenomes characterization and phylogenetic analyses of Ceratophyllus anisus and Leptopsylla segnis

Fleas are one of the most common ectoparasites in warm-blooded mammals and an important vector of zoonotic diseases with serious medical implications. We sequenced the complete mitochondrial genomes of Ceratophyllus anisus and Leptopsylla segnis for the first time using high-throughput sequencing an...

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Autores principales: Liu, Yafang, Chen, Bin, Lu, Xinyan, Jiang, Dandan, Wang, Tao, Geng, Ling, Zhang, Quanfu, Yang, Xing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312373/
https://www.ncbi.nlm.nih.gov/pubmed/37396998
http://dx.doi.org/10.3389/fvets.2023.1218488
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author Liu, Yafang
Chen, Bin
Lu, Xinyan
Jiang, Dandan
Wang, Tao
Geng, Ling
Zhang, Quanfu
Yang, Xing
author_facet Liu, Yafang
Chen, Bin
Lu, Xinyan
Jiang, Dandan
Wang, Tao
Geng, Ling
Zhang, Quanfu
Yang, Xing
author_sort Liu, Yafang
collection PubMed
description Fleas are one of the most common ectoparasites in warm-blooded mammals and an important vector of zoonotic diseases with serious medical implications. We sequenced the complete mitochondrial genomes of Ceratophyllus anisus and Leptopsylla segnis for the first time using high-throughput sequencing and constructed phylogenetic relationships. We obtained double-stranded circular molecules of lengths 15,875 and 15,785 bp, respectively, consisting of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, and two control regions. AT-skew was negative in both C. anisus (−0.022) and L. segnis (−0.231), while GC-skew was positive in both (0.024/0.248), which produced significant differences in codon usage and amino acid composition. Thirteen PCGs encoding 3,617 and 3,711 codons, respectively, isoleucine and phenylalanine were used most frequently. The tRNA genes all form a typical secondary structure. Construction of phylogenetic trees based on Bayesian inference (BI) and maximum likelihood (ML) methods for PCGs. The results of this study provide new information for the mitochondrial genome database of fleas and support further taxonomic studies and population genetics of fleas.
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spelling pubmed-103123732023-07-01 Complete mitogenomes characterization and phylogenetic analyses of Ceratophyllus anisus and Leptopsylla segnis Liu, Yafang Chen, Bin Lu, Xinyan Jiang, Dandan Wang, Tao Geng, Ling Zhang, Quanfu Yang, Xing Front Vet Sci Veterinary Science Fleas are one of the most common ectoparasites in warm-blooded mammals and an important vector of zoonotic diseases with serious medical implications. We sequenced the complete mitochondrial genomes of Ceratophyllus anisus and Leptopsylla segnis for the first time using high-throughput sequencing and constructed phylogenetic relationships. We obtained double-stranded circular molecules of lengths 15,875 and 15,785 bp, respectively, consisting of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, and two control regions. AT-skew was negative in both C. anisus (−0.022) and L. segnis (−0.231), while GC-skew was positive in both (0.024/0.248), which produced significant differences in codon usage and amino acid composition. Thirteen PCGs encoding 3,617 and 3,711 codons, respectively, isoleucine and phenylalanine were used most frequently. The tRNA genes all form a typical secondary structure. Construction of phylogenetic trees based on Bayesian inference (BI) and maximum likelihood (ML) methods for PCGs. The results of this study provide new information for the mitochondrial genome database of fleas and support further taxonomic studies and population genetics of fleas. Frontiers Media S.A. 2023-06-16 /pmc/articles/PMC10312373/ /pubmed/37396998 http://dx.doi.org/10.3389/fvets.2023.1218488 Text en Copyright © 2023 Liu, Chen, Lu, Jiang, Wang, Geng, Zhang and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Liu, Yafang
Chen, Bin
Lu, Xinyan
Jiang, Dandan
Wang, Tao
Geng, Ling
Zhang, Quanfu
Yang, Xing
Complete mitogenomes characterization and phylogenetic analyses of Ceratophyllus anisus and Leptopsylla segnis
title Complete mitogenomes characterization and phylogenetic analyses of Ceratophyllus anisus and Leptopsylla segnis
title_full Complete mitogenomes characterization and phylogenetic analyses of Ceratophyllus anisus and Leptopsylla segnis
title_fullStr Complete mitogenomes characterization and phylogenetic analyses of Ceratophyllus anisus and Leptopsylla segnis
title_full_unstemmed Complete mitogenomes characterization and phylogenetic analyses of Ceratophyllus anisus and Leptopsylla segnis
title_short Complete mitogenomes characterization and phylogenetic analyses of Ceratophyllus anisus and Leptopsylla segnis
title_sort complete mitogenomes characterization and phylogenetic analyses of ceratophyllus anisus and leptopsylla segnis
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312373/
https://www.ncbi.nlm.nih.gov/pubmed/37396998
http://dx.doi.org/10.3389/fvets.2023.1218488
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