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N-glycans show distinct spatial distribution in mouse brain

Protein N-linked glycosylation is a ubiquitous modification in the secretory pathway that plays a critical role in the development and function of the brain. N-glycans have a distinct composition and undergo tight regulation in the brain, but the spatial distribution of these structures remains rela...

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Autores principales: Noel, Maxence, Cummings, Richard D., Mealer, Robert G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312599/
https://www.ncbi.nlm.nih.gov/pubmed/37398169
http://dx.doi.org/10.1101/2023.05.30.542954
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author Noel, Maxence
Cummings, Richard D.
Mealer, Robert G.
author_facet Noel, Maxence
Cummings, Richard D.
Mealer, Robert G.
author_sort Noel, Maxence
collection PubMed
description Protein N-linked glycosylation is a ubiquitous modification in the secretory pathway that plays a critical role in the development and function of the brain. N-glycans have a distinct composition and undergo tight regulation in the brain, but the spatial distribution of these structures remains relatively unexplored. Here, we systematically employed carbohydrate binding lectins with differing specificities to various classes of N-glycans and appropriate controls to identify multiple regions of the mouse brain. Lectins binding high-mannose-type N-glycans, the most abundant class of brain N-glycans, showed diffuse staining with some punctate structures observed on high magnification. Lectins binding specific motifs of complex N-glycans, including fucose and bisecting GlcNAc, showed more partitioned labeling, including to the synapse-rich molecular layer of the cerebellum. Understanding the distribution of N-glycans across the brain will aid future studies of these critical protein modifications in development and disease of the brain.
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spelling pubmed-103125992023-07-01 N-glycans show distinct spatial distribution in mouse brain Noel, Maxence Cummings, Richard D. Mealer, Robert G. bioRxiv Article Protein N-linked glycosylation is a ubiquitous modification in the secretory pathway that plays a critical role in the development and function of the brain. N-glycans have a distinct composition and undergo tight regulation in the brain, but the spatial distribution of these structures remains relatively unexplored. Here, we systematically employed carbohydrate binding lectins with differing specificities to various classes of N-glycans and appropriate controls to identify multiple regions of the mouse brain. Lectins binding high-mannose-type N-glycans, the most abundant class of brain N-glycans, showed diffuse staining with some punctate structures observed on high magnification. Lectins binding specific motifs of complex N-glycans, including fucose and bisecting GlcNAc, showed more partitioned labeling, including to the synapse-rich molecular layer of the cerebellum. Understanding the distribution of N-glycans across the brain will aid future studies of these critical protein modifications in development and disease of the brain. Cold Spring Harbor Laboratory 2023-05-30 /pmc/articles/PMC10312599/ /pubmed/37398169 http://dx.doi.org/10.1101/2023.05.30.542954 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Noel, Maxence
Cummings, Richard D.
Mealer, Robert G.
N-glycans show distinct spatial distribution in mouse brain
title N-glycans show distinct spatial distribution in mouse brain
title_full N-glycans show distinct spatial distribution in mouse brain
title_fullStr N-glycans show distinct spatial distribution in mouse brain
title_full_unstemmed N-glycans show distinct spatial distribution in mouse brain
title_short N-glycans show distinct spatial distribution in mouse brain
title_sort n-glycans show distinct spatial distribution in mouse brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312599/
https://www.ncbi.nlm.nih.gov/pubmed/37398169
http://dx.doi.org/10.1101/2023.05.30.542954
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