Scalable, cell type-selective, AAV-based in vivo CRISPR screening in the mouse brain

CRISPR-based genetic screening directly in mammalian tissues in vivo is challenging due to the need for scalable, cell-type selective delivery and recovery of guide RNA libraries. We developed an in vivo adeno-associated virus-based and Cre recombinase-dependent workflow for cell type-selective CRIS...

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Autores principales: Ramani, Biswarathan, Rose, Indigo V.L., Pan, Andrew, Tian, Ruilin, Ma, Keran, Palop, Jorge J., Kampmann, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312723/
https://www.ncbi.nlm.nih.gov/pubmed/37398301
http://dx.doi.org/10.1101/2023.06.13.544831
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author Ramani, Biswarathan
Rose, Indigo V.L.
Pan, Andrew
Tian, Ruilin
Ma, Keran
Palop, Jorge J.
Kampmann, Martin
author_facet Ramani, Biswarathan
Rose, Indigo V.L.
Pan, Andrew
Tian, Ruilin
Ma, Keran
Palop, Jorge J.
Kampmann, Martin
author_sort Ramani, Biswarathan
collection PubMed
description CRISPR-based genetic screening directly in mammalian tissues in vivo is challenging due to the need for scalable, cell-type selective delivery and recovery of guide RNA libraries. We developed an in vivo adeno-associated virus-based and Cre recombinase-dependent workflow for cell type-selective CRISPR interference screening in mouse tissues. We demonstrate the power of this approach by identifying neuron-essential genes in the mouse brain using a library targeting over 2000 genes.
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spelling pubmed-103127232023-07-01 Scalable, cell type-selective, AAV-based in vivo CRISPR screening in the mouse brain Ramani, Biswarathan Rose, Indigo V.L. Pan, Andrew Tian, Ruilin Ma, Keran Palop, Jorge J. Kampmann, Martin bioRxiv Article CRISPR-based genetic screening directly in mammalian tissues in vivo is challenging due to the need for scalable, cell-type selective delivery and recovery of guide RNA libraries. We developed an in vivo adeno-associated virus-based and Cre recombinase-dependent workflow for cell type-selective CRISPR interference screening in mouse tissues. We demonstrate the power of this approach by identifying neuron-essential genes in the mouse brain using a library targeting over 2000 genes. Cold Spring Harbor Laboratory 2023-06-27 /pmc/articles/PMC10312723/ /pubmed/37398301 http://dx.doi.org/10.1101/2023.06.13.544831 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Ramani, Biswarathan
Rose, Indigo V.L.
Pan, Andrew
Tian, Ruilin
Ma, Keran
Palop, Jorge J.
Kampmann, Martin
Scalable, cell type-selective, AAV-based in vivo CRISPR screening in the mouse brain
title Scalable, cell type-selective, AAV-based in vivo CRISPR screening in the mouse brain
title_full Scalable, cell type-selective, AAV-based in vivo CRISPR screening in the mouse brain
title_fullStr Scalable, cell type-selective, AAV-based in vivo CRISPR screening in the mouse brain
title_full_unstemmed Scalable, cell type-selective, AAV-based in vivo CRISPR screening in the mouse brain
title_short Scalable, cell type-selective, AAV-based in vivo CRISPR screening in the mouse brain
title_sort scalable, cell type-selective, aav-based in vivo crispr screening in the mouse brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312723/
https://www.ncbi.nlm.nih.gov/pubmed/37398301
http://dx.doi.org/10.1101/2023.06.13.544831
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