Scalable, cell type-selective, AAV-based in vivo CRISPR screening in the mouse brain

CRISPR-based genetic screening directly in mammalian tissues in vivo is challenging due to the need for scalable, cell-type selective delivery and recovery of guide RNA libraries. We developed an in vivo adeno-associated virus-based and Cre recombinase-dependent workflow for cell type-selective CRIS...

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Detalles Bibliográficos
Autores principales: Ramani, Biswarathan, Rose, Indigo V.L., Pan, Andrew, Tian, Ruilin, Ma, Keran, Palop, Jorge J., Kampmann, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312723/
https://www.ncbi.nlm.nih.gov/pubmed/37398301
http://dx.doi.org/10.1101/2023.06.13.544831

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312723/
https://www.ncbi.nlm.nih.gov/pubmed/37398301
http://dx.doi.org/10.1101/2023.06.13.544831

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