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Denitrification contributes to N(2)O emission in paddy soils

Denitrification is vital to nitrogen removal and N(2)O release in ecosystems; in this regard, paddy soils exhibit strong denitrifying ability. However, the underlying mechanism of N(2)O emission from denitrification in paddy soils is yet to be elucidated. In this study, the potential N(2)O emission...

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Autores principales: Xiang, Hua, Hong, Yiguo, Wu, Jiapeng, Wang, Yu, Ye, Fei, Ye, Jiaqi, Lu, Jing, Long, Aimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10313071/
https://www.ncbi.nlm.nih.gov/pubmed/37396352
http://dx.doi.org/10.3389/fmicb.2023.1218207
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author Xiang, Hua
Hong, Yiguo
Wu, Jiapeng
Wang, Yu
Ye, Fei
Ye, Jiaqi
Lu, Jing
Long, Aimin
author_facet Xiang, Hua
Hong, Yiguo
Wu, Jiapeng
Wang, Yu
Ye, Fei
Ye, Jiaqi
Lu, Jing
Long, Aimin
author_sort Xiang, Hua
collection PubMed
description Denitrification is vital to nitrogen removal and N(2)O release in ecosystems; in this regard, paddy soils exhibit strong denitrifying ability. However, the underlying mechanism of N(2)O emission from denitrification in paddy soils is yet to be elucidated. In this study, the potential N(2)O emission rate, enzymatic activity for N(2)O production and reduction, gene abundance, and community composition during denitrification were investigated using the (15)N isotope tracer technique combined with slurry incubation, enzymatic activity detection, quantitative polymerase chain reaction (qPCR), and metagenomic sequencing. Results of incubation experiments showed that the average potential N(2)O emission rates were 0.51 ± 0.20 μmol⋅N⋅kg(–1)⋅h(–1), which constituted 2.16 ± 0.85% of the denitrification end-products. The enzymatic activity for N(2)O production was 2.77–8.94 times than that for N(2)O reduction, indicating an imbalance between N(2)O production and reduction. The gene abundance ratio of nir to nosZ from qPCR results further supported the imbalance. Results of metagenomic analysis showed that, although Proteobacteria was the common phylum for denitrification genes, other dominant community compositions varied for different denitrification genes. Gammaproteobacteria and other phyla containing the norB gene without nosZ genes, including Actinobacteria, Planctomycetes, Desulfobacterota, Cyanobacteria, Acidobacteria, Bacteroidetes, and Myxococcus, may contribute to N(2)O emission from paddy soils. Our results suggest that denitrification is highly modular, with different microbial communities collaborating to complete the denitrification process, thus resulting in an emission estimation of 13.67 ± 5.44 g N(2)O⋅m(–2)⋅yr(–1) in surface paddy soils.
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spelling pubmed-103130712023-07-01 Denitrification contributes to N(2)O emission in paddy soils Xiang, Hua Hong, Yiguo Wu, Jiapeng Wang, Yu Ye, Fei Ye, Jiaqi Lu, Jing Long, Aimin Front Microbiol Microbiology Denitrification is vital to nitrogen removal and N(2)O release in ecosystems; in this regard, paddy soils exhibit strong denitrifying ability. However, the underlying mechanism of N(2)O emission from denitrification in paddy soils is yet to be elucidated. In this study, the potential N(2)O emission rate, enzymatic activity for N(2)O production and reduction, gene abundance, and community composition during denitrification were investigated using the (15)N isotope tracer technique combined with slurry incubation, enzymatic activity detection, quantitative polymerase chain reaction (qPCR), and metagenomic sequencing. Results of incubation experiments showed that the average potential N(2)O emission rates were 0.51 ± 0.20 μmol⋅N⋅kg(–1)⋅h(–1), which constituted 2.16 ± 0.85% of the denitrification end-products. The enzymatic activity for N(2)O production was 2.77–8.94 times than that for N(2)O reduction, indicating an imbalance between N(2)O production and reduction. The gene abundance ratio of nir to nosZ from qPCR results further supported the imbalance. Results of metagenomic analysis showed that, although Proteobacteria was the common phylum for denitrification genes, other dominant community compositions varied for different denitrification genes. Gammaproteobacteria and other phyla containing the norB gene without nosZ genes, including Actinobacteria, Planctomycetes, Desulfobacterota, Cyanobacteria, Acidobacteria, Bacteroidetes, and Myxococcus, may contribute to N(2)O emission from paddy soils. Our results suggest that denitrification is highly modular, with different microbial communities collaborating to complete the denitrification process, thus resulting in an emission estimation of 13.67 ± 5.44 g N(2)O⋅m(–2)⋅yr(–1) in surface paddy soils. Frontiers Media S.A. 2023-06-16 /pmc/articles/PMC10313071/ /pubmed/37396352 http://dx.doi.org/10.3389/fmicb.2023.1218207 Text en Copyright © 2023 Xiang, Hong, Wu, Wang, Ye, Ye, Lu and Long. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xiang, Hua
Hong, Yiguo
Wu, Jiapeng
Wang, Yu
Ye, Fei
Ye, Jiaqi
Lu, Jing
Long, Aimin
Denitrification contributes to N(2)O emission in paddy soils
title Denitrification contributes to N(2)O emission in paddy soils
title_full Denitrification contributes to N(2)O emission in paddy soils
title_fullStr Denitrification contributes to N(2)O emission in paddy soils
title_full_unstemmed Denitrification contributes to N(2)O emission in paddy soils
title_short Denitrification contributes to N(2)O emission in paddy soils
title_sort denitrification contributes to n(2)o emission in paddy soils
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10313071/
https://www.ncbi.nlm.nih.gov/pubmed/37396352
http://dx.doi.org/10.3389/fmicb.2023.1218207
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