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Technical note on the exploration of COVID-19 in autopsy material

Interrogation of immune response in autopsy material from patients with SARS-CoV-2 is potentially significant. We aim to describe a validated protocol for the exploration of the molecular physiopathology of SARS-CoV-2 pulmonary disease using multiplex immunofluorescence (mIF). The application of val...

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Autores principales: Humphries, Matthew Phillip, Bingham, Victoria, Abdullah Sidi, Fatima, Craig, Stephanie, Lara, Beatrize, El-daly, Hesham, O'Doherty, Nicole, Maxwell, Perry, Lewis, Claire, McQuaid, Stephen, Lyness, James, James, Jacqueline, Snead, David R J, Salto-Tellez, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10313984/
https://www.ncbi.nlm.nih.gov/pubmed/36717223
http://dx.doi.org/10.1136/jcp-2022-208525
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author Humphries, Matthew Phillip
Bingham, Victoria
Abdullah Sidi, Fatima
Craig, Stephanie
Lara, Beatrize
El-daly, Hesham
O'Doherty, Nicole
Maxwell, Perry
Lewis, Claire
McQuaid, Stephen
Lyness, James
James, Jacqueline
Snead, David R J
Salto-Tellez, Manuel
author_facet Humphries, Matthew Phillip
Bingham, Victoria
Abdullah Sidi, Fatima
Craig, Stephanie
Lara, Beatrize
El-daly, Hesham
O'Doherty, Nicole
Maxwell, Perry
Lewis, Claire
McQuaid, Stephen
Lyness, James
James, Jacqueline
Snead, David R J
Salto-Tellez, Manuel
author_sort Humphries, Matthew Phillip
collection PubMed
description Interrogation of immune response in autopsy material from patients with SARS-CoV-2 is potentially significant. We aim to describe a validated protocol for the exploration of the molecular physiopathology of SARS-CoV-2 pulmonary disease using multiplex immunofluorescence (mIF). The application of validated assays for the detection of SARS-CoV-2 in tissues, originally developed in our laboratory in the context of oncology, was used to map the topography and complexity of the adaptive immune response at protein and mRNA levels. SARS-CoV-2 is detectable in situ by protein or mRNA, with a sensitivity that could be in part related to disease stage. In formalin-fixed, paraffin-embedded pneumonia material, multiplex immunofluorescent panels are robust, reliable and quantifiable and can detect topographic variations in inflammation related to pathological processes. Clinical autopsies have relevance in understanding diseases of unknown/complex pathophysiology. In particular, autopsy materials are suitable for the detection of SARS-CoV-2 and for the topographic description of the complex tissue-based immune response using mIF.
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spelling pubmed-103139842023-07-02 Technical note on the exploration of COVID-19 in autopsy material Humphries, Matthew Phillip Bingham, Victoria Abdullah Sidi, Fatima Craig, Stephanie Lara, Beatrize El-daly, Hesham O'Doherty, Nicole Maxwell, Perry Lewis, Claire McQuaid, Stephen Lyness, James James, Jacqueline Snead, David R J Salto-Tellez, Manuel J Clin Pathol Short Report Interrogation of immune response in autopsy material from patients with SARS-CoV-2 is potentially significant. We aim to describe a validated protocol for the exploration of the molecular physiopathology of SARS-CoV-2 pulmonary disease using multiplex immunofluorescence (mIF). The application of validated assays for the detection of SARS-CoV-2 in tissues, originally developed in our laboratory in the context of oncology, was used to map the topography and complexity of the adaptive immune response at protein and mRNA levels. SARS-CoV-2 is detectable in situ by protein or mRNA, with a sensitivity that could be in part related to disease stage. In formalin-fixed, paraffin-embedded pneumonia material, multiplex immunofluorescent panels are robust, reliable and quantifiable and can detect topographic variations in inflammation related to pathological processes. Clinical autopsies have relevance in understanding diseases of unknown/complex pathophysiology. In particular, autopsy materials are suitable for the detection of SARS-CoV-2 and for the topographic description of the complex tissue-based immune response using mIF. BMJ Publishing Group 2023-06 2023-01-30 /pmc/articles/PMC10313984/ /pubmed/36717223 http://dx.doi.org/10.1136/jcp-2022-208525 Text en © Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY. Published by BMJ. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/.
spellingShingle Short Report
Humphries, Matthew Phillip
Bingham, Victoria
Abdullah Sidi, Fatima
Craig, Stephanie
Lara, Beatrize
El-daly, Hesham
O'Doherty, Nicole
Maxwell, Perry
Lewis, Claire
McQuaid, Stephen
Lyness, James
James, Jacqueline
Snead, David R J
Salto-Tellez, Manuel
Technical note on the exploration of COVID-19 in autopsy material
title Technical note on the exploration of COVID-19 in autopsy material
title_full Technical note on the exploration of COVID-19 in autopsy material
title_fullStr Technical note on the exploration of COVID-19 in autopsy material
title_full_unstemmed Technical note on the exploration of COVID-19 in autopsy material
title_short Technical note on the exploration of COVID-19 in autopsy material
title_sort technical note on the exploration of covid-19 in autopsy material
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10313984/
https://www.ncbi.nlm.nih.gov/pubmed/36717223
http://dx.doi.org/10.1136/jcp-2022-208525
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