Comparison of neuronal GAL4 drivers along with the AGES (auxin-inducible gene expression system) and TARGET (temporal and regional gene expression targeting) systems for fine tuning of neuronal gene expression in Drosophila

Spatial and temporal control of gene expression in Drosophila is essential in elucidating gene function. Spatial control is facilitated by the UAS/GAL4 system, and this can be coupled with additional adaptations for precise temporal control and fine tuning of gene expression levels. Here we directly...

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Autores principales: Hawley, Hannah R, Roberts, Celestine J, Fitzsimons, Helen L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314298/
https://www.ncbi.nlm.nih.gov/pubmed/37396791
http://dx.doi.org/10.17912/micropub.biology.000885
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author Hawley, Hannah R
Roberts, Celestine J
Fitzsimons, Helen L
author_facet Hawley, Hannah R
Roberts, Celestine J
Fitzsimons, Helen L
author_sort Hawley, Hannah R
collection PubMed
description Spatial and temporal control of gene expression in Drosophila is essential in elucidating gene function. Spatial control is facilitated by the UAS/GAL4 system, and this can be coupled with additional adaptations for precise temporal control and fine tuning of gene expression levels. Here we directly compare the level of pan-neuronal transgene expression governed by nSyb-GAL4 and elav-GAL4, as well as mushroom body-specific expression alongside OK107-GAL4. We also compare the temporal modulation of gene expression in neurons with the auxin-inducible gene expression system (AGES) and temporal and regional gene expression targeting (TARGET) systems.
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spelling pubmed-103142982023-07-02 Comparison of neuronal GAL4 drivers along with the AGES (auxin-inducible gene expression system) and TARGET (temporal and regional gene expression targeting) systems for fine tuning of neuronal gene expression in Drosophila Hawley, Hannah R Roberts, Celestine J Fitzsimons, Helen L MicroPubl Biol Materials and Reagents Spatial and temporal control of gene expression in Drosophila is essential in elucidating gene function. Spatial control is facilitated by the UAS/GAL4 system, and this can be coupled with additional adaptations for precise temporal control and fine tuning of gene expression levels. Here we directly compare the level of pan-neuronal transgene expression governed by nSyb-GAL4 and elav-GAL4, as well as mushroom body-specific expression alongside OK107-GAL4. We also compare the temporal modulation of gene expression in neurons with the auxin-inducible gene expression system (AGES) and temporal and regional gene expression targeting (TARGET) systems. Caltech Library 2023-06-16 /pmc/articles/PMC10314298/ /pubmed/37396791 http://dx.doi.org/10.17912/micropub.biology.000885 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Materials and Reagents
Hawley, Hannah R
Roberts, Celestine J
Fitzsimons, Helen L
Comparison of neuronal GAL4 drivers along with the AGES (auxin-inducible gene expression system) and TARGET (temporal and regional gene expression targeting) systems for fine tuning of neuronal gene expression in Drosophila
title Comparison of neuronal GAL4 drivers along with the AGES (auxin-inducible gene expression system) and TARGET (temporal and regional gene expression targeting) systems for fine tuning of neuronal gene expression in Drosophila
title_full Comparison of neuronal GAL4 drivers along with the AGES (auxin-inducible gene expression system) and TARGET (temporal and regional gene expression targeting) systems for fine tuning of neuronal gene expression in Drosophila
title_fullStr Comparison of neuronal GAL4 drivers along with the AGES (auxin-inducible gene expression system) and TARGET (temporal and regional gene expression targeting) systems for fine tuning of neuronal gene expression in Drosophila
title_full_unstemmed Comparison of neuronal GAL4 drivers along with the AGES (auxin-inducible gene expression system) and TARGET (temporal and regional gene expression targeting) systems for fine tuning of neuronal gene expression in Drosophila
title_short Comparison of neuronal GAL4 drivers along with the AGES (auxin-inducible gene expression system) and TARGET (temporal and regional gene expression targeting) systems for fine tuning of neuronal gene expression in Drosophila
title_sort comparison of neuronal gal4 drivers along with the ages (auxin-inducible gene expression system) and target (temporal and regional gene expression targeting) systems for fine tuning of neuronal gene expression in drosophila
topic Materials and Reagents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314298/
https://www.ncbi.nlm.nih.gov/pubmed/37396791
http://dx.doi.org/10.17912/micropub.biology.000885
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