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Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development
BACKGROUND: Conserved polycomb repressive complex 2 (PRC2) mediates H3K27me3 to direct transcriptional repression and has a key role in cell fate determination and cell differentiation in both animals and plants. PRC2 subunits have undergone independent multiplication and functional divergence in hi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314529/ https://www.ncbi.nlm.nih.gov/pubmed/37391710 http://dx.doi.org/10.1186/s12870-023-04359-9 |
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author | Zhu, Tianqing Hu, Jiwen Yang, Xiaowei Kong, Lisheng Ling, Juanjuan Wang, Junhui An, Sanping |
author_facet | Zhu, Tianqing Hu, Jiwen Yang, Xiaowei Kong, Lisheng Ling, Juanjuan Wang, Junhui An, Sanping |
author_sort | Zhu, Tianqing |
collection | PubMed |
description | BACKGROUND: Conserved polycomb repressive complex 2 (PRC2) mediates H3K27me3 to direct transcriptional repression and has a key role in cell fate determination and cell differentiation in both animals and plants. PRC2 subunits have undergone independent multiplication and functional divergence in higher plants. However, relevant information is still absent in gymnosperms. RESULTS: To launch gymnosperm PRC2 research, we identified and cloned the PRC2 core component genes in the conifer model species Picea abies, including one Esc/FIE homolog PaFIE, two p55/MSI homologs PaMSI1a and PaMSI1b, two E(z) homologs PaKMT6A2 and PaKMT6A4, a Su(z)12 homolog PaEMF2 and a PaEMF2-like fragment. Phylogenetic and protein domain analyses were conducted. The Esc/FIE homologs were highly conserved in the land plant, except the monocots. The other gymnospermous PRC2 subunits underwent independent evolution with angiospermous species to different extents. The relative transcript levels of these genes were measured in endosperm and zygotic and somatic embryos at different developmental stages. The obtained results proposed the involvement of PaMSI1b and PaKMT6A4 in embryogenesis and PaKMT6A2 and PaEMF2 in the transition from embryos to seedlings. The PaEMF2-like fragment was predominantly expressed in the endosperm but not in the embryo. In addition, immunohistochemistry assay showed that H3K27me3 deposits were generally enriched at meristem regions during seed development in P. abies. CONCLUSIONS: This study reports the first characterization of the PRC2 core component genes in the coniferous species P. abies. Our work may enable a deeper understanding of the cell reprogramming process during seed and embryo development and may guide further research on embryonic potential and development in conifers. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04359-9. |
format | Online Article Text |
id | pubmed-10314529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-103145292023-07-02 Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development Zhu, Tianqing Hu, Jiwen Yang, Xiaowei Kong, Lisheng Ling, Juanjuan Wang, Junhui An, Sanping BMC Plant Biol Research BACKGROUND: Conserved polycomb repressive complex 2 (PRC2) mediates H3K27me3 to direct transcriptional repression and has a key role in cell fate determination and cell differentiation in both animals and plants. PRC2 subunits have undergone independent multiplication and functional divergence in higher plants. However, relevant information is still absent in gymnosperms. RESULTS: To launch gymnosperm PRC2 research, we identified and cloned the PRC2 core component genes in the conifer model species Picea abies, including one Esc/FIE homolog PaFIE, two p55/MSI homologs PaMSI1a and PaMSI1b, two E(z) homologs PaKMT6A2 and PaKMT6A4, a Su(z)12 homolog PaEMF2 and a PaEMF2-like fragment. Phylogenetic and protein domain analyses were conducted. The Esc/FIE homologs were highly conserved in the land plant, except the monocots. The other gymnospermous PRC2 subunits underwent independent evolution with angiospermous species to different extents. The relative transcript levels of these genes were measured in endosperm and zygotic and somatic embryos at different developmental stages. The obtained results proposed the involvement of PaMSI1b and PaKMT6A4 in embryogenesis and PaKMT6A2 and PaEMF2 in the transition from embryos to seedlings. The PaEMF2-like fragment was predominantly expressed in the endosperm but not in the embryo. In addition, immunohistochemistry assay showed that H3K27me3 deposits were generally enriched at meristem regions during seed development in P. abies. CONCLUSIONS: This study reports the first characterization of the PRC2 core component genes in the coniferous species P. abies. Our work may enable a deeper understanding of the cell reprogramming process during seed and embryo development and may guide further research on embryonic potential and development in conifers. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04359-9. BioMed Central 2023-07-01 /pmc/articles/PMC10314529/ /pubmed/37391710 http://dx.doi.org/10.1186/s12870-023-04359-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zhu, Tianqing Hu, Jiwen Yang, Xiaowei Kong, Lisheng Ling, Juanjuan Wang, Junhui An, Sanping Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development |
title | Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development |
title_full | Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development |
title_fullStr | Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development |
title_full_unstemmed | Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development |
title_short | Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development |
title_sort | analysis of polycomb repressive complex 2 (prc2) subunits in picea abies with a focus on embryo development |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314529/ https://www.ncbi.nlm.nih.gov/pubmed/37391710 http://dx.doi.org/10.1186/s12870-023-04359-9 |
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