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Effect of Mir-4270 Inhibitor and Mimic on Viability and Stemness in Gastric Cancer Stem-Like Cells Derived from MKN-45 Cell Line
BACKGROUND: MiRNAs are significant regulatory factors in stem cell proliferation, and alteration in miRNA expression influences the viability and gene expression of cancer stem cells. Herein, we evaluated the effect of the hsa-miR-4270 inhibitor and mimic on the expression of stem cell markers in GC...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Pasteur Institute of Iran
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314761/ https://www.ncbi.nlm.nih.gov/pubmed/37070617 http://dx.doi.org/10.52547/ibj.3851 |
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author | Akrami, Hassan Shamsdin, Seyedeh Azra Nikmanesh, Yousef Fattahi, Mohammadreza |
author_facet | Akrami, Hassan Shamsdin, Seyedeh Azra Nikmanesh, Yousef Fattahi, Mohammadreza |
author_sort | Akrami, Hassan |
collection | PubMed |
description | BACKGROUND: MiRNAs are significant regulatory factors in stem cell proliferation, and alteration in miRNA expression influences the viability and gene expression of cancer stem cells. Herein, we evaluated the effect of the hsa-miR-4270 inhibitor and mimic on the expression of stem cell markers in GCSCs. METHODS: GCSCs were isolated from the MKN-45 cell line by a non-adherent surface system. The cells were confirmed by differentiation assays using dexamethasone and insulin as adipogenesis-inducing agents and also staurosporine as a neural-inducing agent. Isolated GCSCs were treated with different concentrations (0, 15, 20, 25, 30, 40, 50, and 60 nM) of hsa-miR-4270 inhibitor and mimic. The cell viability was determined by Trypan blue method. Transcription of the stem cell marker genes, including CD44, OCT3/4, SOX2, Nanog, and KLF4, was evaluated by quantitative real-time RT-PCR. RESULTS: The results showed that GCSCs were differentiated into adipose cells using dexamethasone and insulin, and neural cells by staurosporine. Treatment of the GCSCs with hsa-miR-4270 inhibitor decreased the cell viability and downregulated OCT3/4, CD44, and Nanog genes to 86%, 79%, and 91% respectively. Also, SOX2 and KLF4 were overexpressed to 8.1- and 1.94-folds, respectively. However, hsa-miR-4270 mimic had opposite effects on the cell viability and gene expression of the stem cell markers. CONCLUSION: Effect of hsa-miR-4270 inhibitor and mimic on the expression of the stem cell markers in GCSCs indicates that hsa-miR-4270 stimulates the stemness property of GCSCs, likely through stimulating the development of gastric stem cells. |
format | Online Article Text |
id | pubmed-10314761 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Pasteur Institute of Iran |
record_format | MEDLINE/PubMed |
spelling | pubmed-103147612023-07-02 Effect of Mir-4270 Inhibitor and Mimic on Viability and Stemness in Gastric Cancer Stem-Like Cells Derived from MKN-45 Cell Line Akrami, Hassan Shamsdin, Seyedeh Azra Nikmanesh, Yousef Fattahi, Mohammadreza Iran Biomed J Full Length BACKGROUND: MiRNAs are significant regulatory factors in stem cell proliferation, and alteration in miRNA expression influences the viability and gene expression of cancer stem cells. Herein, we evaluated the effect of the hsa-miR-4270 inhibitor and mimic on the expression of stem cell markers in GCSCs. METHODS: GCSCs were isolated from the MKN-45 cell line by a non-adherent surface system. The cells were confirmed by differentiation assays using dexamethasone and insulin as adipogenesis-inducing agents and also staurosporine as a neural-inducing agent. Isolated GCSCs were treated with different concentrations (0, 15, 20, 25, 30, 40, 50, and 60 nM) of hsa-miR-4270 inhibitor and mimic. The cell viability was determined by Trypan blue method. Transcription of the stem cell marker genes, including CD44, OCT3/4, SOX2, Nanog, and KLF4, was evaluated by quantitative real-time RT-PCR. RESULTS: The results showed that GCSCs were differentiated into adipose cells using dexamethasone and insulin, and neural cells by staurosporine. Treatment of the GCSCs with hsa-miR-4270 inhibitor decreased the cell viability and downregulated OCT3/4, CD44, and Nanog genes to 86%, 79%, and 91% respectively. Also, SOX2 and KLF4 were overexpressed to 8.1- and 1.94-folds, respectively. However, hsa-miR-4270 mimic had opposite effects on the cell viability and gene expression of the stem cell markers. CONCLUSION: Effect of hsa-miR-4270 inhibitor and mimic on the expression of the stem cell markers in GCSCs indicates that hsa-miR-4270 stimulates the stemness property of GCSCs, likely through stimulating the development of gastric stem cells. Pasteur Institute of Iran 2023 2023-01-23 /pmc/articles/PMC10314761/ /pubmed/37070617 http://dx.doi.org/10.52547/ibj.3851 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Length Akrami, Hassan Shamsdin, Seyedeh Azra Nikmanesh, Yousef Fattahi, Mohammadreza Effect of Mir-4270 Inhibitor and Mimic on Viability and Stemness in Gastric Cancer Stem-Like Cells Derived from MKN-45 Cell Line |
title | Effect of Mir-4270 Inhibitor and Mimic on Viability and Stemness in Gastric Cancer Stem-Like Cells Derived from MKN-45 Cell Line |
title_full | Effect of Mir-4270 Inhibitor and Mimic on Viability and Stemness in Gastric Cancer Stem-Like Cells Derived from MKN-45 Cell Line |
title_fullStr | Effect of Mir-4270 Inhibitor and Mimic on Viability and Stemness in Gastric Cancer Stem-Like Cells Derived from MKN-45 Cell Line |
title_full_unstemmed | Effect of Mir-4270 Inhibitor and Mimic on Viability and Stemness in Gastric Cancer Stem-Like Cells Derived from MKN-45 Cell Line |
title_short | Effect of Mir-4270 Inhibitor and Mimic on Viability and Stemness in Gastric Cancer Stem-Like Cells Derived from MKN-45 Cell Line |
title_sort | effect of mir-4270 inhibitor and mimic on viability and stemness in gastric cancer stem-like cells derived from mkn-45 cell line |
topic | Full Length |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314761/ https://www.ncbi.nlm.nih.gov/pubmed/37070617 http://dx.doi.org/10.52547/ibj.3851 |
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