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K-Ras4A Plays a More Significant Role than K-Ras4B in Ductal Carcinoma of Breast

BACKGROUND: K-Ras mutations rarely occur in breast cancer. However, studies have supported that K-Ras upregulation is involved in breast cancer pathogenesis. Two main K-Ras transcript variants, K-Ras4A and K-Ras4B, arise originate from the alternative splicing of exon 4. In this study, we aimed to e...

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Autores principales: Mortazavipour, Mohamad Mahdi, Mohamadalizadeh-Hanjani, Zeinab, Geranpayeh, Loabat, Mahdian, Reza, Shahbazi, Shirin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute of Iran 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314762/
https://www.ncbi.nlm.nih.gov/pubmed/37070675
http://dx.doi.org/10.52547/ibj.3857
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author Mortazavipour, Mohamad Mahdi
Mohamadalizadeh-Hanjani, Zeinab
Geranpayeh, Loabat
Mahdian, Reza
Shahbazi, Shirin
author_facet Mortazavipour, Mohamad Mahdi
Mohamadalizadeh-Hanjani, Zeinab
Geranpayeh, Loabat
Mahdian, Reza
Shahbazi, Shirin
author_sort Mortazavipour, Mohamad Mahdi
collection PubMed
description BACKGROUND: K-Ras mutations rarely occur in breast cancer. However, studies have supported that K-Ras upregulation is involved in breast cancer pathogenesis. Two main K-Ras transcript variants, K-Ras4A and K-Ras4B, arise originate from the alternative splicing of exon 4. In this study, we aimed to evaluate variations in the expression of K-Ras4A and K-Ras4B and their role in breast ductal carcinoma. METHODS: Total RNA was extracted from breast tumors, and the NATs were obtained via mastectomy. Patients were selected from new cases of breast cancer with no prior history of chemotherapy. Relative mRNA expression was calculated based on a pairwise comparison between the tumors and the NATs following normalization to the internal control gene. Predictive values of the transcript variants were examined by ROC curve analysis. RESULTS: A statistically significant increase was found in K-Ras4A and K-Ras4B expression with the mean fold changes of 7.58 (p = 0.01) and 2.47 (p = 0.001), respectively. The K-Ras4A/K-Ras4B ratio was lower in the tumors than that of the normal tissues. ROC curve analysis revealed the potential of K-Ras4A (AUC: 0.769) and K-Ras4B (AUC: 0.688) in breast cancer prediction. There was also a significant association between K-Ras4B expression and HER2 statues (p = 0.04). Furthermore, a significant link was detected between K-Ras4A expression and pathological prognostic stages (p = 0.04). CONCLUSION: Our findings reveal that the expression levels of K-Ras4A and K-Ras4B is higher in the tumor compared to the normal breast tissues. Increase in K-Ras4A expression was more significant than that of K-Ras4B.
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spelling pubmed-103147622023-07-02 K-Ras4A Plays a More Significant Role than K-Ras4B in Ductal Carcinoma of Breast Mortazavipour, Mohamad Mahdi Mohamadalizadeh-Hanjani, Zeinab Geranpayeh, Loabat Mahdian, Reza Shahbazi, Shirin Iran Biomed J Full Length BACKGROUND: K-Ras mutations rarely occur in breast cancer. However, studies have supported that K-Ras upregulation is involved in breast cancer pathogenesis. Two main K-Ras transcript variants, K-Ras4A and K-Ras4B, arise originate from the alternative splicing of exon 4. In this study, we aimed to evaluate variations in the expression of K-Ras4A and K-Ras4B and their role in breast ductal carcinoma. METHODS: Total RNA was extracted from breast tumors, and the NATs were obtained via mastectomy. Patients were selected from new cases of breast cancer with no prior history of chemotherapy. Relative mRNA expression was calculated based on a pairwise comparison between the tumors and the NATs following normalization to the internal control gene. Predictive values of the transcript variants were examined by ROC curve analysis. RESULTS: A statistically significant increase was found in K-Ras4A and K-Ras4B expression with the mean fold changes of 7.58 (p = 0.01) and 2.47 (p = 0.001), respectively. The K-Ras4A/K-Ras4B ratio was lower in the tumors than that of the normal tissues. ROC curve analysis revealed the potential of K-Ras4A (AUC: 0.769) and K-Ras4B (AUC: 0.688) in breast cancer prediction. There was also a significant association between K-Ras4B expression and HER2 statues (p = 0.04). Furthermore, a significant link was detected between K-Ras4A expression and pathological prognostic stages (p = 0.04). CONCLUSION: Our findings reveal that the expression levels of K-Ras4A and K-Ras4B is higher in the tumor compared to the normal breast tissues. Increase in K-Ras4A expression was more significant than that of K-Ras4B. Pasteur Institute of Iran 2023 2023-01-01 /pmc/articles/PMC10314762/ /pubmed/37070675 http://dx.doi.org/10.52547/ibj.3857 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Length
Mortazavipour, Mohamad Mahdi
Mohamadalizadeh-Hanjani, Zeinab
Geranpayeh, Loabat
Mahdian, Reza
Shahbazi, Shirin
K-Ras4A Plays a More Significant Role than K-Ras4B in Ductal Carcinoma of Breast
title K-Ras4A Plays a More Significant Role than K-Ras4B in Ductal Carcinoma of Breast
title_full K-Ras4A Plays a More Significant Role than K-Ras4B in Ductal Carcinoma of Breast
title_fullStr K-Ras4A Plays a More Significant Role than K-Ras4B in Ductal Carcinoma of Breast
title_full_unstemmed K-Ras4A Plays a More Significant Role than K-Ras4B in Ductal Carcinoma of Breast
title_short K-Ras4A Plays a More Significant Role than K-Ras4B in Ductal Carcinoma of Breast
title_sort k-ras4a plays a more significant role than k-ras4b in ductal carcinoma of breast
topic Full Length
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314762/
https://www.ncbi.nlm.nih.gov/pubmed/37070675
http://dx.doi.org/10.52547/ibj.3857
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