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Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens

Waterfowl, such as ducks, are natural hosts for avian influenza viruses (AIVs) and act as a bridge for transmitting the virus to humans or susceptible chickens. Since 2013, chickens and ducks have been threatened by waterfowl-origin H5N6 subtype AIVs in China. Therefore, it is necessary to investiga...

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Autores principales: He, Zhuoliang, Wang, Xia, Lin, Yu, Feng, Siyu, Huang, Xinyu, Zhao, Luxiang, Zhang, Junsheng, Ding, Yangbao, Li, Weiqiang, Yuan, Runyu, Jiao, Peirong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10315182/
https://www.ncbi.nlm.nih.gov/pubmed/37405154
http://dx.doi.org/10.3389/fmicb.2023.1211355
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author He, Zhuoliang
Wang, Xia
Lin, Yu
Feng, Siyu
Huang, Xinyu
Zhao, Luxiang
Zhang, Junsheng
Ding, Yangbao
Li, Weiqiang
Yuan, Runyu
Jiao, Peirong
author_facet He, Zhuoliang
Wang, Xia
Lin, Yu
Feng, Siyu
Huang, Xinyu
Zhao, Luxiang
Zhang, Junsheng
Ding, Yangbao
Li, Weiqiang
Yuan, Runyu
Jiao, Peirong
author_sort He, Zhuoliang
collection PubMed
description Waterfowl, such as ducks, are natural hosts for avian influenza viruses (AIVs) and act as a bridge for transmitting the virus to humans or susceptible chickens. Since 2013, chickens and ducks have been threatened by waterfowl-origin H5N6 subtype AIVs in China. Therefore, it is necessary to investigate the genetic evolution, transmission, and pathogenicity of these viruses. In this study, we determined the genetic characteristics, transmission, and pathogenicity of waterfowl-origin H5N6 viruses in southern China. The hemagglutinin (HA) genes of H5N6 viruses were classified into the MIX-like branch of clade 2.3.4.4h. The neuraminidase (NA) genes belonged to the Eurasian lineage. The PB1 genes were classified into MIX-like and VN 2014-like branches. The remaining five genes were clustered into the MIX-like branch. Therefore, these viruses belonged to different genotypes. The cleavage site of the HA proteins of these viruses was RERRRKR/G, a molecular characteristic of the H5 highly pathogenic AIV. The NA stalk of all H5N6 viruses contained 11 amino acid deletions at residues 58–68. All viruses contained 627E and 701D in the PB2 proteins, which were molecular characteristics of typical bird AIVs. Furthermore, this study showed that Q135 and S23 viruses could replicate systematically in chickens and ducks. They did not cause death in ducks but induced mild clinical signs in them. All the infected chickens showed severe clinical signs and died. These viruses were shed from the digestive and respiratory tracts and transmitted horizontally in chickens and ducks. Our results provide valuable information for preventing H5N6 avian influenza outbreaks.
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spelling pubmed-103151822023-07-03 Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens He, Zhuoliang Wang, Xia Lin, Yu Feng, Siyu Huang, Xinyu Zhao, Luxiang Zhang, Junsheng Ding, Yangbao Li, Weiqiang Yuan, Runyu Jiao, Peirong Front Microbiol Microbiology Waterfowl, such as ducks, are natural hosts for avian influenza viruses (AIVs) and act as a bridge for transmitting the virus to humans or susceptible chickens. Since 2013, chickens and ducks have been threatened by waterfowl-origin H5N6 subtype AIVs in China. Therefore, it is necessary to investigate the genetic evolution, transmission, and pathogenicity of these viruses. In this study, we determined the genetic characteristics, transmission, and pathogenicity of waterfowl-origin H5N6 viruses in southern China. The hemagglutinin (HA) genes of H5N6 viruses were classified into the MIX-like branch of clade 2.3.4.4h. The neuraminidase (NA) genes belonged to the Eurasian lineage. The PB1 genes were classified into MIX-like and VN 2014-like branches. The remaining five genes were clustered into the MIX-like branch. Therefore, these viruses belonged to different genotypes. The cleavage site of the HA proteins of these viruses was RERRRKR/G, a molecular characteristic of the H5 highly pathogenic AIV. The NA stalk of all H5N6 viruses contained 11 amino acid deletions at residues 58–68. All viruses contained 627E and 701D in the PB2 proteins, which were molecular characteristics of typical bird AIVs. Furthermore, this study showed that Q135 and S23 viruses could replicate systematically in chickens and ducks. They did not cause death in ducks but induced mild clinical signs in them. All the infected chickens showed severe clinical signs and died. These viruses were shed from the digestive and respiratory tracts and transmitted horizontally in chickens and ducks. Our results provide valuable information for preventing H5N6 avian influenza outbreaks. Frontiers Media S.A. 2023-06-15 /pmc/articles/PMC10315182/ /pubmed/37405154 http://dx.doi.org/10.3389/fmicb.2023.1211355 Text en Copyright © 2023 He, Wang, Lin, Feng, Huang, Zhao, Zhang, Ding, Li, Yuan and Jiao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
He, Zhuoliang
Wang, Xia
Lin, Yu
Feng, Siyu
Huang, Xinyu
Zhao, Luxiang
Zhang, Junsheng
Ding, Yangbao
Li, Weiqiang
Yuan, Runyu
Jiao, Peirong
Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens
title Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens
title_full Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens
title_fullStr Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens
title_full_unstemmed Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens
title_short Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens
title_sort genetic characteristics of waterfowl-origin h5n6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10315182/
https://www.ncbi.nlm.nih.gov/pubmed/37405154
http://dx.doi.org/10.3389/fmicb.2023.1211355
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