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Study on diversity, nitrogen-fixing capacity, and heavy metal tolerance of culturable Pongamia pinnata rhizobia in the vanadium-titanium magnetite tailings

INTRODUCTION: The diversity, nitrogen-fixing capacity and heavy metal tolerance of culturable rhizobia in symbiotic relationship with Pongamia pinnata surviving in vanadium (V) - titanium (Ti) magnetite (VTM) tailings is still unknown, and the rhizobia isolates from the extreme barren VTM tailings c...

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Autores principales: Shen, Tian, Jin, Ruimin, Yan, Jing, Cheng, Xiran, Zeng, Lan, Chen, Qiang, Gu, Yunfu, Zou, Likou, Zhao, Ke, Xiang, Quanju, Penttinen, Petri, Ma, Menggen, Li, Shuangcheng, Zou, Ting, Yu, Xiumei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10315665/
https://www.ncbi.nlm.nih.gov/pubmed/37405163
http://dx.doi.org/10.3389/fmicb.2023.1078333
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author Shen, Tian
Jin, Ruimin
Yan, Jing
Cheng, Xiran
Zeng, Lan
Chen, Qiang
Gu, Yunfu
Zou, Likou
Zhao, Ke
Xiang, Quanju
Penttinen, Petri
Ma, Menggen
Li, Shuangcheng
Zou, Ting
Yu, Xiumei
author_facet Shen, Tian
Jin, Ruimin
Yan, Jing
Cheng, Xiran
Zeng, Lan
Chen, Qiang
Gu, Yunfu
Zou, Likou
Zhao, Ke
Xiang, Quanju
Penttinen, Petri
Ma, Menggen
Li, Shuangcheng
Zou, Ting
Yu, Xiumei
author_sort Shen, Tian
collection PubMed
description INTRODUCTION: The diversity, nitrogen-fixing capacity and heavy metal tolerance of culturable rhizobia in symbiotic relationship with Pongamia pinnata surviving in vanadium (V) - titanium (Ti) magnetite (VTM) tailings is still unknown, and the rhizobia isolates from the extreme barren VTM tailings contaminated with a variety of metals would provide available rhizobia resources for bioremediation. METHODS: P. pinnata plants were cultivated in pots containing the VTM tailings until root nodules formed, and then culturable rhizobia were isolated from root nodules. The diversity, nitrogen-fixing capacity and heavy metal tolerance of rhizobia were performed. RESULTS: Among 57 rhizobia isolated from these nodules, only twenty strains showed different levels of tolerance to copper (Cu), nickel (Ni), manganese (Mn) and zinc (Zn), especially strains PP1 and PP76 showing high tolerance against these four heavy metals. Based on the phylogenetic analysis of 16S rRNA and four house-keeping genes (atpD, recA, rpoB, glnII), twelve isolates were identified as Bradyrhizobium pachyrhizi, four as Ochrobactrum anthropic, three as Rhizobium selenitireducens and one as Rhizobium pisi. Some rhizobia isolates showed a high nitrogen-fixing capacity and promoted P. pinnata growth by increasing nitrogen content by 10%-145% in aboveground plant part and 13%-79% in the root. R. pachyrhizi PP1 showed the strongest capacity of nitrogen fixation, plant growth promotion and resistance to heavy metals, which provided effective rhizobia strains for bioremediation of VTM tailings or other contaminated soils. This study demonstrated that there are at least three genera of culturable rhizobia in symbiosis with P. pinnata in VTM tailings. DISCUSSION: Abundant culturable rhizobia with the capacity of nitrogen fixation, plant growth promotion and resistance to heavy metals survived in VTM tailings, indicating more valuable functional microbes could be isolated from extreme soil environments such as VTM tailings.
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spelling pubmed-103156652023-07-04 Study on diversity, nitrogen-fixing capacity, and heavy metal tolerance of culturable Pongamia pinnata rhizobia in the vanadium-titanium magnetite tailings Shen, Tian Jin, Ruimin Yan, Jing Cheng, Xiran Zeng, Lan Chen, Qiang Gu, Yunfu Zou, Likou Zhao, Ke Xiang, Quanju Penttinen, Petri Ma, Menggen Li, Shuangcheng Zou, Ting Yu, Xiumei Front Microbiol Microbiology INTRODUCTION: The diversity, nitrogen-fixing capacity and heavy metal tolerance of culturable rhizobia in symbiotic relationship with Pongamia pinnata surviving in vanadium (V) - titanium (Ti) magnetite (VTM) tailings is still unknown, and the rhizobia isolates from the extreme barren VTM tailings contaminated with a variety of metals would provide available rhizobia resources for bioremediation. METHODS: P. pinnata plants were cultivated in pots containing the VTM tailings until root nodules formed, and then culturable rhizobia were isolated from root nodules. The diversity, nitrogen-fixing capacity and heavy metal tolerance of rhizobia were performed. RESULTS: Among 57 rhizobia isolated from these nodules, only twenty strains showed different levels of tolerance to copper (Cu), nickel (Ni), manganese (Mn) and zinc (Zn), especially strains PP1 and PP76 showing high tolerance against these four heavy metals. Based on the phylogenetic analysis of 16S rRNA and four house-keeping genes (atpD, recA, rpoB, glnII), twelve isolates were identified as Bradyrhizobium pachyrhizi, four as Ochrobactrum anthropic, three as Rhizobium selenitireducens and one as Rhizobium pisi. Some rhizobia isolates showed a high nitrogen-fixing capacity and promoted P. pinnata growth by increasing nitrogen content by 10%-145% in aboveground plant part and 13%-79% in the root. R. pachyrhizi PP1 showed the strongest capacity of nitrogen fixation, plant growth promotion and resistance to heavy metals, which provided effective rhizobia strains for bioremediation of VTM tailings or other contaminated soils. This study demonstrated that there are at least three genera of culturable rhizobia in symbiosis with P. pinnata in VTM tailings. DISCUSSION: Abundant culturable rhizobia with the capacity of nitrogen fixation, plant growth promotion and resistance to heavy metals survived in VTM tailings, indicating more valuable functional microbes could be isolated from extreme soil environments such as VTM tailings. Frontiers Media S.A. 2023-06-19 /pmc/articles/PMC10315665/ /pubmed/37405163 http://dx.doi.org/10.3389/fmicb.2023.1078333 Text en Copyright © 2023 Shen, Jin, Yan, Cheng, Zeng, Chen, Gu, Zou, Zhao, Xiang, Penttinen, Ma, Li, Zou and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Shen, Tian
Jin, Ruimin
Yan, Jing
Cheng, Xiran
Zeng, Lan
Chen, Qiang
Gu, Yunfu
Zou, Likou
Zhao, Ke
Xiang, Quanju
Penttinen, Petri
Ma, Menggen
Li, Shuangcheng
Zou, Ting
Yu, Xiumei
Study on diversity, nitrogen-fixing capacity, and heavy metal tolerance of culturable Pongamia pinnata rhizobia in the vanadium-titanium magnetite tailings
title Study on diversity, nitrogen-fixing capacity, and heavy metal tolerance of culturable Pongamia pinnata rhizobia in the vanadium-titanium magnetite tailings
title_full Study on diversity, nitrogen-fixing capacity, and heavy metal tolerance of culturable Pongamia pinnata rhizobia in the vanadium-titanium magnetite tailings
title_fullStr Study on diversity, nitrogen-fixing capacity, and heavy metal tolerance of culturable Pongamia pinnata rhizobia in the vanadium-titanium magnetite tailings
title_full_unstemmed Study on diversity, nitrogen-fixing capacity, and heavy metal tolerance of culturable Pongamia pinnata rhizobia in the vanadium-titanium magnetite tailings
title_short Study on diversity, nitrogen-fixing capacity, and heavy metal tolerance of culturable Pongamia pinnata rhizobia in the vanadium-titanium magnetite tailings
title_sort study on diversity, nitrogen-fixing capacity, and heavy metal tolerance of culturable pongamia pinnata rhizobia in the vanadium-titanium magnetite tailings
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10315665/
https://www.ncbi.nlm.nih.gov/pubmed/37405163
http://dx.doi.org/10.3389/fmicb.2023.1078333
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