Cargando…

In vitro Antibacterial Activity and Wound Healing Effects of Achillea millefolium Essential Oil in Rat

OBJECTIVES: In this study we aimed to evaluate the in vitro antibacterial activity and wound healing properties of Achillea millefolium essential oil (AMEO) in full-thickness wound model in rat. The antibacterial activity of AMEO was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa...

Descripción completa

Detalles Bibliográficos
Autores principales: Ghasemi, Mohammad Reza, Ranjbar, Akram, Tamri, Pari, Pourmoslemi, Shabnam, Nourian, Alireza, Dastan, Dara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Pharmacopuncture Institute (KPI) 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10315885/
https://www.ncbi.nlm.nih.gov/pubmed/37405118
http://dx.doi.org/10.3831/KPI.2023.26.2.167
_version_ 1785067596542902272
author Ghasemi, Mohammad Reza
Ranjbar, Akram
Tamri, Pari
Pourmoslemi, Shabnam
Nourian, Alireza
Dastan, Dara
author_facet Ghasemi, Mohammad Reza
Ranjbar, Akram
Tamri, Pari
Pourmoslemi, Shabnam
Nourian, Alireza
Dastan, Dara
author_sort Ghasemi, Mohammad Reza
collection PubMed
description OBJECTIVES: In this study we aimed to evaluate the in vitro antibacterial activity and wound healing properties of Achillea millefolium essential oil (AMEO) in full-thickness wound model in rat. The antibacterial activity of AMEO was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa using the broth dilution method. METHODS: The 2 cm × 2 cm full-thickness excisional wounds were created on the back of animals. Topical therapy was applied twice a day using 1%, 2%, and 3% w/w AMEO ointments, and the measurement of the wounds area was carried out every 3 days, after that the wound closure percentage was calculated in these days. Hydroxyproline content and histopathological evaluation of wound tissue samples were carried out on day 7 and 14 post wounding. Eucerin was used for the treatment of vehicle control group and negative control group received no treatment. RESULTS: Our results revealed the bacteriostatic activity of AMEO against S. aureus and P. aeruginosa. Wound healing activity evaluation of AMEO showed the significant increase (p < 0.05) in the wound closure percentages in rats treated with AMEO 1% and 2% comparing to those of non-treatment group. In addition, hydroxyproline contents of tissue significantly (p < 0.01) increased in AMEO 1% and 2% comparing to non-treatment group. Histopathological evaluations of wound tissue samples on day 7 and 14 demonstrated higher accumulation of collagen fibers, reduction of edema and inflammation and also formation of tissue appendages in 1% and 2% AMEO treated groups in comparison with non-treatment group. CONCLUSION: The results of this study indicated that AMEO has the potential to be used as a safe and effective wound healing agent.
format Online
Article
Text
id pubmed-10315885
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher The Korean Pharmacopuncture Institute (KPI)
record_format MEDLINE/PubMed
spelling pubmed-103158852023-07-04 In vitro Antibacterial Activity and Wound Healing Effects of Achillea millefolium Essential Oil in Rat Ghasemi, Mohammad Reza Ranjbar, Akram Tamri, Pari Pourmoslemi, Shabnam Nourian, Alireza Dastan, Dara J Pharmacopuncture Original Article OBJECTIVES: In this study we aimed to evaluate the in vitro antibacterial activity and wound healing properties of Achillea millefolium essential oil (AMEO) in full-thickness wound model in rat. The antibacterial activity of AMEO was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa using the broth dilution method. METHODS: The 2 cm × 2 cm full-thickness excisional wounds were created on the back of animals. Topical therapy was applied twice a day using 1%, 2%, and 3% w/w AMEO ointments, and the measurement of the wounds area was carried out every 3 days, after that the wound closure percentage was calculated in these days. Hydroxyproline content and histopathological evaluation of wound tissue samples were carried out on day 7 and 14 post wounding. Eucerin was used for the treatment of vehicle control group and negative control group received no treatment. RESULTS: Our results revealed the bacteriostatic activity of AMEO against S. aureus and P. aeruginosa. Wound healing activity evaluation of AMEO showed the significant increase (p < 0.05) in the wound closure percentages in rats treated with AMEO 1% and 2% comparing to those of non-treatment group. In addition, hydroxyproline contents of tissue significantly (p < 0.01) increased in AMEO 1% and 2% comparing to non-treatment group. Histopathological evaluations of wound tissue samples on day 7 and 14 demonstrated higher accumulation of collagen fibers, reduction of edema and inflammation and also formation of tissue appendages in 1% and 2% AMEO treated groups in comparison with non-treatment group. CONCLUSION: The results of this study indicated that AMEO has the potential to be used as a safe and effective wound healing agent. The Korean Pharmacopuncture Institute (KPI) 2023-06-30 2023-06-30 /pmc/articles/PMC10315885/ /pubmed/37405118 http://dx.doi.org/10.3831/KPI.2023.26.2.167 Text en © 2023 Korean Pharmacopuncture Institute https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ghasemi, Mohammad Reza
Ranjbar, Akram
Tamri, Pari
Pourmoslemi, Shabnam
Nourian, Alireza
Dastan, Dara
In vitro Antibacterial Activity and Wound Healing Effects of Achillea millefolium Essential Oil in Rat
title In vitro Antibacterial Activity and Wound Healing Effects of Achillea millefolium Essential Oil in Rat
title_full In vitro Antibacterial Activity and Wound Healing Effects of Achillea millefolium Essential Oil in Rat
title_fullStr In vitro Antibacterial Activity and Wound Healing Effects of Achillea millefolium Essential Oil in Rat
title_full_unstemmed In vitro Antibacterial Activity and Wound Healing Effects of Achillea millefolium Essential Oil in Rat
title_short In vitro Antibacterial Activity and Wound Healing Effects of Achillea millefolium Essential Oil in Rat
title_sort in vitro antibacterial activity and wound healing effects of achillea millefolium essential oil in rat
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10315885/
https://www.ncbi.nlm.nih.gov/pubmed/37405118
http://dx.doi.org/10.3831/KPI.2023.26.2.167
work_keys_str_mv AT ghasemimohammadreza invitroantibacterialactivityandwoundhealingeffectsofachilleamillefoliumessentialoilinrat
AT ranjbarakram invitroantibacterialactivityandwoundhealingeffectsofachilleamillefoliumessentialoilinrat
AT tamripari invitroantibacterialactivityandwoundhealingeffectsofachilleamillefoliumessentialoilinrat
AT pourmoslemishabnam invitroantibacterialactivityandwoundhealingeffectsofachilleamillefoliumessentialoilinrat
AT nourianalireza invitroantibacterialactivityandwoundhealingeffectsofachilleamillefoliumessentialoilinrat
AT dastandara invitroantibacterialactivityandwoundhealingeffectsofachilleamillefoliumessentialoilinrat