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Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study

BACKGROUND: This study evaluated if genetic variations in the WNT family members and RUNX2 are associated with craniofacial maturation, investigating dental and skeletal maturity in children and teenagers. METHODS: Radiographs from pre-orthodontic treatment of Brazilian patients (7 to 17 years-old)...

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Autores principales: Bitencourt Reis, Caio Luiz, Nakane Matsumoto, Mirian Aiko, Baratto-Filho, Flares, Scariot, Rafaela, Sasso Stuani, Maria Bernadete, Lourenço Romano, Fábio, Della Coletta, Ricardo, Silva Barroso de Oliveira, Daniela, Proff, Peter, Kirschneck, Christian, Calvano Küchler, Erika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10316614/
https://www.ncbi.nlm.nih.gov/pubmed/37400934
http://dx.doi.org/10.1186/s13005-023-00372-3
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author Bitencourt Reis, Caio Luiz
Nakane Matsumoto, Mirian Aiko
Baratto-Filho, Flares
Scariot, Rafaela
Sasso Stuani, Maria Bernadete
Lourenço Romano, Fábio
Della Coletta, Ricardo
Silva Barroso de Oliveira, Daniela
Proff, Peter
Kirschneck, Christian
Calvano Küchler, Erika
author_facet Bitencourt Reis, Caio Luiz
Nakane Matsumoto, Mirian Aiko
Baratto-Filho, Flares
Scariot, Rafaela
Sasso Stuani, Maria Bernadete
Lourenço Romano, Fábio
Della Coletta, Ricardo
Silva Barroso de Oliveira, Daniela
Proff, Peter
Kirschneck, Christian
Calvano Küchler, Erika
author_sort Bitencourt Reis, Caio Luiz
collection PubMed
description BACKGROUND: This study evaluated if genetic variations in the WNT family members and RUNX2 are associated with craniofacial maturation, investigating dental and skeletal maturity in children and teenagers. METHODS: Radiographs from pre-orthodontic treatment of Brazilian patients (7 to 17 years-old) were used to assess dental (panoramic radiographs) and skeletal maturity (cephalometric radiographs). The chronological age (CA) was calculated based on the date of birth and the time the radiographs were performed. For the dental maturity analysis, the Demirjian (1973) method was used and a delta [dental age - chronological age (DA-CA)] was calculated. For the skeletal maturity analysis, the Baccetti et al. (2005) method was used and the patients were classified as “delayed skeletal maturation”, “advanced skeletal maturation” or “normal skeletal maturation”. DNA isolated from buccal cells was used for genotyping of two genetic variations in WNT family genes: rs708111 (G > A) in WNT3A and rs1533767 (G > A) in WNT11; and two genetic variations in RUNX2: rs1200425 (G > A) and rs59983488 (G > T). A statistical analysis was performed and values of p < 0.05 indicated a significant difference. RESULTS: There were no associations between dental maturity and genotypes (p > 0.05). In the skeletal maturity analysis, the allele A in the rs708111 (WNT3A) was statistically more frequent in patients with delayed skeletal maturation (Prevalence Ratio = 1.6; 95% Confidence Interval = 1.00 to 2.54; p-value = 0.042). CONCLUSIONS: The rs708111 in the WNT3A gene impacts on skeletal maturation.
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spelling pubmed-103166142023-07-04 Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study Bitencourt Reis, Caio Luiz Nakane Matsumoto, Mirian Aiko Baratto-Filho, Flares Scariot, Rafaela Sasso Stuani, Maria Bernadete Lourenço Romano, Fábio Della Coletta, Ricardo Silva Barroso de Oliveira, Daniela Proff, Peter Kirschneck, Christian Calvano Küchler, Erika Head Face Med Research BACKGROUND: This study evaluated if genetic variations in the WNT family members and RUNX2 are associated with craniofacial maturation, investigating dental and skeletal maturity in children and teenagers. METHODS: Radiographs from pre-orthodontic treatment of Brazilian patients (7 to 17 years-old) were used to assess dental (panoramic radiographs) and skeletal maturity (cephalometric radiographs). The chronological age (CA) was calculated based on the date of birth and the time the radiographs were performed. For the dental maturity analysis, the Demirjian (1973) method was used and a delta [dental age - chronological age (DA-CA)] was calculated. For the skeletal maturity analysis, the Baccetti et al. (2005) method was used and the patients were classified as “delayed skeletal maturation”, “advanced skeletal maturation” or “normal skeletal maturation”. DNA isolated from buccal cells was used for genotyping of two genetic variations in WNT family genes: rs708111 (G > A) in WNT3A and rs1533767 (G > A) in WNT11; and two genetic variations in RUNX2: rs1200425 (G > A) and rs59983488 (G > T). A statistical analysis was performed and values of p < 0.05 indicated a significant difference. RESULTS: There were no associations between dental maturity and genotypes (p > 0.05). In the skeletal maturity analysis, the allele A in the rs708111 (WNT3A) was statistically more frequent in patients with delayed skeletal maturation (Prevalence Ratio = 1.6; 95% Confidence Interval = 1.00 to 2.54; p-value = 0.042). CONCLUSIONS: The rs708111 in the WNT3A gene impacts on skeletal maturation. BioMed Central 2023-07-03 /pmc/articles/PMC10316614/ /pubmed/37400934 http://dx.doi.org/10.1186/s13005-023-00372-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Bitencourt Reis, Caio Luiz
Nakane Matsumoto, Mirian Aiko
Baratto-Filho, Flares
Scariot, Rafaela
Sasso Stuani, Maria Bernadete
Lourenço Romano, Fábio
Della Coletta, Ricardo
Silva Barroso de Oliveira, Daniela
Proff, Peter
Kirschneck, Christian
Calvano Küchler, Erika
Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study
title Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study
title_full Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study
title_fullStr Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study
title_full_unstemmed Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study
title_short Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study
title_sort impact of genetic variations in the wnt family members and runx2 on dental and skeletal maturation: a cross-sectional study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10316614/
https://www.ncbi.nlm.nih.gov/pubmed/37400934
http://dx.doi.org/10.1186/s13005-023-00372-3
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