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Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone?
Objective To analyze the effects of estrogen alone or in combination with progestogens and tibolone (TIB) on the expression of the extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), of perlecan, and of heparanase (HPSE) of the vascular walls of the carotid arteries. Methods A total o...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Thieme Revinter Publicações Ltda
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10316798/ https://www.ncbi.nlm.nih.gov/pubmed/31344719 http://dx.doi.org/10.1055/s-0039-1693681 |
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author | Steiner, Marcelo Luis Theodoro, Thérèse Rachell Garcia, Shirley Gimenez Mader, Ana Maria Amaral Antonio Pompei, Luciano de Melo Pinhal, Maria Aparecida da Silva Fernandes, César Eduardo |
author_facet | Steiner, Marcelo Luis Theodoro, Thérèse Rachell Garcia, Shirley Gimenez Mader, Ana Maria Amaral Antonio Pompei, Luciano de Melo Pinhal, Maria Aparecida da Silva Fernandes, César Eduardo |
author_sort | Steiner, Marcelo Luis |
collection | PubMed |
description | Objective To analyze the effects of estrogen alone or in combination with progestogens and tibolone (TIB) on the expression of the extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), of perlecan, and of heparanase (HPSE) of the vascular walls of the carotid arteries. Methods A total of 30 250-day-old ovariectomized Wistar rats were orally treated for 5 weeks with: a) 1 mg/kg of estradiol benzoate (EB); b) EB + 0.2 mg/kg of medroxyprogesterone acetate (MPA); c) EB + 0.2mg/kg of norethisterone acetate (NETA); d) EB + 2 mg/kg of dydrogesterone (DI); e) 1 mg/kg of TIB; f) placebo (CTR). Following treatment, the expression of mRNA for MMP-2, MMP-9, and HPSE was analyzed by real-time polymerase chain-reaction (PCR), and the expression of MMP-2, of MMP-9, of tissue inhibitor of metalloproteinase 2 (TIMP-2), and of perlecan was quantified by immunohistochemistry in the carotid arteries. Results The groups showed significant differences on mRNA HPSE expression (p = 0.048), which was higher in the EB, EB + MPA, and TIB groups. There was no statistically significant difference in mRNA MMP-2 or MMP-9 expression. The immunohistochemical expression of MMP-2, of TIMP-2, of MMP-9, of HPSE, and of perlecan showed no differences between groups. Conclusion Estradiol alone or associated with MPA and TIB treatment can increase mRNA HSPE expression of the walls of the carotid arteries in ovariectomized rats. |
format | Online Article Text |
id | pubmed-10316798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Thieme Revinter Publicações Ltda |
record_format | MEDLINE/PubMed |
spelling | pubmed-103167982023-07-27 Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? Steiner, Marcelo Luis Theodoro, Thérèse Rachell Garcia, Shirley Gimenez Mader, Ana Maria Amaral Antonio Pompei, Luciano de Melo Pinhal, Maria Aparecida da Silva Fernandes, César Eduardo Rev Bras Ginecol Obstet Objective To analyze the effects of estrogen alone or in combination with progestogens and tibolone (TIB) on the expression of the extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), of perlecan, and of heparanase (HPSE) of the vascular walls of the carotid arteries. Methods A total of 30 250-day-old ovariectomized Wistar rats were orally treated for 5 weeks with: a) 1 mg/kg of estradiol benzoate (EB); b) EB + 0.2 mg/kg of medroxyprogesterone acetate (MPA); c) EB + 0.2mg/kg of norethisterone acetate (NETA); d) EB + 2 mg/kg of dydrogesterone (DI); e) 1 mg/kg of TIB; f) placebo (CTR). Following treatment, the expression of mRNA for MMP-2, MMP-9, and HPSE was analyzed by real-time polymerase chain-reaction (PCR), and the expression of MMP-2, of MMP-9, of tissue inhibitor of metalloproteinase 2 (TIMP-2), and of perlecan was quantified by immunohistochemistry in the carotid arteries. Results The groups showed significant differences on mRNA HPSE expression (p = 0.048), which was higher in the EB, EB + MPA, and TIB groups. There was no statistically significant difference in mRNA MMP-2 or MMP-9 expression. The immunohistochemical expression of MMP-2, of TIMP-2, of MMP-9, of HPSE, and of perlecan showed no differences between groups. Conclusion Estradiol alone or associated with MPA and TIB treatment can increase mRNA HSPE expression of the walls of the carotid arteries in ovariectomized rats. Thieme Revinter Publicações Ltda 2019-07 /pmc/articles/PMC10316798/ /pubmed/31344719 http://dx.doi.org/10.1055/s-0039-1693681 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Steiner, Marcelo Luis Theodoro, Thérèse Rachell Garcia, Shirley Gimenez Mader, Ana Maria Amaral Antonio Pompei, Luciano de Melo Pinhal, Maria Aparecida da Silva Fernandes, César Eduardo Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? |
title | Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? |
title_full | Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? |
title_fullStr | Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? |
title_full_unstemmed | Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? |
title_short | Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? |
title_sort | is the expression of the components of the carotid matrix of rats influenced by estrogen, progestin and tibolone? |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10316798/ https://www.ncbi.nlm.nih.gov/pubmed/31344719 http://dx.doi.org/10.1055/s-0039-1693681 |
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