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Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone?

Objective To analyze the effects of estrogen alone or in combination with progestogens and tibolone (TIB) on the expression of the extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), of perlecan, and of heparanase (HPSE) of the vascular walls of the carotid arteries. Methods A total o...

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Autores principales: Steiner, Marcelo Luis, Theodoro, Thérèse Rachell, Garcia, Shirley Gimenez, Mader, Ana Maria Amaral Antonio, Pompei, Luciano de Melo, Pinhal, Maria Aparecida da Silva, Fernandes, César Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Thieme Revinter Publicações Ltda 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10316798/
https://www.ncbi.nlm.nih.gov/pubmed/31344719
http://dx.doi.org/10.1055/s-0039-1693681
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author Steiner, Marcelo Luis
Theodoro, Thérèse Rachell
Garcia, Shirley Gimenez
Mader, Ana Maria Amaral Antonio
Pompei, Luciano de Melo
Pinhal, Maria Aparecida da Silva
Fernandes, César Eduardo
author_facet Steiner, Marcelo Luis
Theodoro, Thérèse Rachell
Garcia, Shirley Gimenez
Mader, Ana Maria Amaral Antonio
Pompei, Luciano de Melo
Pinhal, Maria Aparecida da Silva
Fernandes, César Eduardo
author_sort Steiner, Marcelo Luis
collection PubMed
description Objective To analyze the effects of estrogen alone or in combination with progestogens and tibolone (TIB) on the expression of the extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), of perlecan, and of heparanase (HPSE) of the vascular walls of the carotid arteries. Methods A total of 30 250-day-old ovariectomized Wistar rats were orally treated for 5 weeks with: a) 1 mg/kg of estradiol benzoate (EB); b) EB + 0.2 mg/kg of medroxyprogesterone acetate (MPA); c) EB + 0.2mg/kg of norethisterone acetate (NETA); d) EB + 2 mg/kg of dydrogesterone (DI); e) 1 mg/kg of TIB; f) placebo (CTR). Following treatment, the expression of mRNA for MMP-2, MMP-9, and HPSE was analyzed by real-time polymerase chain-reaction (PCR), and the expression of MMP-2, of MMP-9, of tissue inhibitor of metalloproteinase 2 (TIMP-2), and of perlecan was quantified by immunohistochemistry in the carotid arteries. Results The groups showed significant differences on mRNA HPSE expression (p = 0.048), which was higher in the EB, EB + MPA, and TIB groups. There was no statistically significant difference in mRNA MMP-2 or MMP-9 expression. The immunohistochemical expression of MMP-2, of TIMP-2, of MMP-9, of HPSE, and of perlecan showed no differences between groups. Conclusion Estradiol alone or associated with MPA and TIB treatment can increase mRNA HSPE expression of the walls of the carotid arteries in ovariectomized rats.
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spelling pubmed-103167982023-07-27 Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? Steiner, Marcelo Luis Theodoro, Thérèse Rachell Garcia, Shirley Gimenez Mader, Ana Maria Amaral Antonio Pompei, Luciano de Melo Pinhal, Maria Aparecida da Silva Fernandes, César Eduardo Rev Bras Ginecol Obstet Objective To analyze the effects of estrogen alone or in combination with progestogens and tibolone (TIB) on the expression of the extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), of perlecan, and of heparanase (HPSE) of the vascular walls of the carotid arteries. Methods A total of 30 250-day-old ovariectomized Wistar rats were orally treated for 5 weeks with: a) 1 mg/kg of estradiol benzoate (EB); b) EB + 0.2 mg/kg of medroxyprogesterone acetate (MPA); c) EB + 0.2mg/kg of norethisterone acetate (NETA); d) EB + 2 mg/kg of dydrogesterone (DI); e) 1 mg/kg of TIB; f) placebo (CTR). Following treatment, the expression of mRNA for MMP-2, MMP-9, and HPSE was analyzed by real-time polymerase chain-reaction (PCR), and the expression of MMP-2, of MMP-9, of tissue inhibitor of metalloproteinase 2 (TIMP-2), and of perlecan was quantified by immunohistochemistry in the carotid arteries. Results The groups showed significant differences on mRNA HPSE expression (p = 0.048), which was higher in the EB, EB + MPA, and TIB groups. There was no statistically significant difference in mRNA MMP-2 or MMP-9 expression. The immunohistochemical expression of MMP-2, of TIMP-2, of MMP-9, of HPSE, and of perlecan showed no differences between groups. Conclusion Estradiol alone or associated with MPA and TIB treatment can increase mRNA HSPE expression of the walls of the carotid arteries in ovariectomized rats. Thieme Revinter Publicações Ltda 2019-07 /pmc/articles/PMC10316798/ /pubmed/31344719 http://dx.doi.org/10.1055/s-0039-1693681 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Steiner, Marcelo Luis
Theodoro, Thérèse Rachell
Garcia, Shirley Gimenez
Mader, Ana Maria Amaral Antonio
Pompei, Luciano de Melo
Pinhal, Maria Aparecida da Silva
Fernandes, César Eduardo
Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone?
title Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone?
title_full Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone?
title_fullStr Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone?
title_full_unstemmed Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone?
title_short Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone?
title_sort is the expression of the components of the carotid matrix of rats influenced by estrogen, progestin and tibolone?
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10316798/
https://www.ncbi.nlm.nih.gov/pubmed/31344719
http://dx.doi.org/10.1055/s-0039-1693681
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