Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics

The intestinal epithelial barrier facilitates homeostatic host–microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for...

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Autores principales: Bootz-Maoz, Hadar, Simon, Ariel, Del Mare-Roumani, Sara, Bennet, Yifat, Toister, Einat, Romano, Hadar, Zheng, Danping, Amidror, Sivan, Elinav, Eran, Yissachar, Nissan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10318059/
https://www.ncbi.nlm.nih.gov/pubmed/37400593
http://dx.doi.org/10.1038/s41522-023-00409-0
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author Bootz-Maoz, Hadar
Simon, Ariel
Del Mare-Roumani, Sara
Bennet, Yifat
Toister, Einat
Romano, Hadar
Zheng, Danping
Amidror, Sivan
Elinav, Eran
Yissachar, Nissan
author_facet Bootz-Maoz, Hadar
Simon, Ariel
Del Mare-Roumani, Sara
Bennet, Yifat
Toister, Einat
Romano, Hadar
Zheng, Danping
Amidror, Sivan
Elinav, Eran
Yissachar, Nissan
author_sort Bootz-Maoz, Hadar
collection PubMed
description The intestinal epithelial barrier facilitates homeostatic host–microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.
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spelling pubmed-103180592023-07-05 Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics Bootz-Maoz, Hadar Simon, Ariel Del Mare-Roumani, Sara Bennet, Yifat Toister, Einat Romano, Hadar Zheng, Danping Amidror, Sivan Elinav, Eran Yissachar, Nissan NPJ Biofilms Microbiomes Brief Communication The intestinal epithelial barrier facilitates homeostatic host–microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions. Nature Publishing Group UK 2023-07-03 /pmc/articles/PMC10318059/ /pubmed/37400593 http://dx.doi.org/10.1038/s41522-023-00409-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Communication
Bootz-Maoz, Hadar
Simon, Ariel
Del Mare-Roumani, Sara
Bennet, Yifat
Toister, Einat
Romano, Hadar
Zheng, Danping
Amidror, Sivan
Elinav, Eran
Yissachar, Nissan
Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics
title Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics
title_full Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics
title_fullStr Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics
title_full_unstemmed Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics
title_short Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics
title_sort ex vivo intestinal permeability assay (x-ipa) for tracking barrier function dynamics
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10318059/
https://www.ncbi.nlm.nih.gov/pubmed/37400593
http://dx.doi.org/10.1038/s41522-023-00409-0
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