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Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L.
Breeding rapeseed varieties with more main inflorescence siliques is an idea for developing rapeseed varieties that are suitable for light and simplified cultivation. The Brassica napus exhibited cluster bud of the main inflorescence (Bnclib) gene. At the fruiting stage, the main inflorescence had m...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10318724/ https://www.ncbi.nlm.nih.gov/pubmed/37403046 http://dx.doi.org/10.1186/s12870-023-04355-z |
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author | Zheng, Wen Yin Zhu, Zhe Yi Sami, Abdul Sun, Meng Yuan Li, Yong Hu, Jian Qian, Xing Zhi Ma, Jin Xu Wang, Mei Qi Yu, Yan Zhang, Fu Gui Zhou, Ke Jin Zhu, Zong He |
author_facet | Zheng, Wen Yin Zhu, Zhe Yi Sami, Abdul Sun, Meng Yuan Li, Yong Hu, Jian Qian, Xing Zhi Ma, Jin Xu Wang, Mei Qi Yu, Yan Zhang, Fu Gui Zhou, Ke Jin Zhu, Zong He |
author_sort | Zheng, Wen Yin |
collection | PubMed |
description | Breeding rapeseed varieties with more main inflorescence siliques is an idea for developing rapeseed varieties that are suitable for light and simplified cultivation. The Brassica napus exhibited cluster bud of the main inflorescence (Bnclib) gene. At the fruiting stage, the main inflorescence had more siliques, higher density, and more main inflorescences. Moreover, the top of the main inflorescence bifurcated. Genetic analysis showed that the separation ratio between Bnclib and the wild type in the F(2) generation was 3:1, which indicated that the trait was a single-gene-dominant inheritance. Among the 24 candidate genes, only one gene, BnaA03g53930D, showed differential expression between the groups (False discovery rate, FDR ≤ 0.05, |log2FC|≤ 1). qPCR verification of the BnaA03g53930D gene between Huyou 17 and its Bnclib near-isogenic line showed that BnaA03g53930D was significantly differentially expressed in the stem tissue of Huyou 17 and its Bnclib near-isogenic line (Bnclib NIL). The determination of gibberellin (GA), brassinolide (BR), cytokinin (CTK), jasmonic acid (JA), growth hormone (IAA), and strigolactone (SL) content in the shoot apex of Huyou 17 by Bnclib NIL and wild type showed that all six hormones significantly differed between the Bnclib NIL and Huyou 17. It is necessary to conduct further research on the interactions between JA and the other five hormones and the main inflorescence bud clustering in B. napus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04355-z. |
format | Online Article Text |
id | pubmed-10318724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-103187242023-07-05 Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. Zheng, Wen Yin Zhu, Zhe Yi Sami, Abdul Sun, Meng Yuan Li, Yong Hu, Jian Qian, Xing Zhi Ma, Jin Xu Wang, Mei Qi Yu, Yan Zhang, Fu Gui Zhou, Ke Jin Zhu, Zong He BMC Plant Biol Research Breeding rapeseed varieties with more main inflorescence siliques is an idea for developing rapeseed varieties that are suitable for light and simplified cultivation. The Brassica napus exhibited cluster bud of the main inflorescence (Bnclib) gene. At the fruiting stage, the main inflorescence had more siliques, higher density, and more main inflorescences. Moreover, the top of the main inflorescence bifurcated. Genetic analysis showed that the separation ratio between Bnclib and the wild type in the F(2) generation was 3:1, which indicated that the trait was a single-gene-dominant inheritance. Among the 24 candidate genes, only one gene, BnaA03g53930D, showed differential expression between the groups (False discovery rate, FDR ≤ 0.05, |log2FC|≤ 1). qPCR verification of the BnaA03g53930D gene between Huyou 17 and its Bnclib near-isogenic line showed that BnaA03g53930D was significantly differentially expressed in the stem tissue of Huyou 17 and its Bnclib near-isogenic line (Bnclib NIL). The determination of gibberellin (GA), brassinolide (BR), cytokinin (CTK), jasmonic acid (JA), growth hormone (IAA), and strigolactone (SL) content in the shoot apex of Huyou 17 by Bnclib NIL and wild type showed that all six hormones significantly differed between the Bnclib NIL and Huyou 17. It is necessary to conduct further research on the interactions between JA and the other five hormones and the main inflorescence bud clustering in B. napus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04355-z. BioMed Central 2023-07-04 /pmc/articles/PMC10318724/ /pubmed/37403046 http://dx.doi.org/10.1186/s12870-023-04355-z Text en © The Author(s) 2023, corrected publication 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zheng, Wen Yin Zhu, Zhe Yi Sami, Abdul Sun, Meng Yuan Li, Yong Hu, Jian Qian, Xing Zhi Ma, Jin Xu Wang, Mei Qi Yu, Yan Zhang, Fu Gui Zhou, Ke Jin Zhu, Zong He Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. |
title | Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. |
title_full | Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. |
title_fullStr | Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. |
title_full_unstemmed | Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. |
title_short | Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. |
title_sort | mapping and candidate gene analysis of clustered bud on the main inflorescence in brassica napus l. |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10318724/ https://www.ncbi.nlm.nih.gov/pubmed/37403046 http://dx.doi.org/10.1186/s12870-023-04355-z |
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