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The Condylar Effects of Mesenchymal Stem Cells, Low-Level Laser Therapy and Grape Seed Extract on Functional Mandibular Advancement of the Rat Mandible
OBJECTIVE: Functional treatment of Class II malocclusion is expected to lead to adaptation in the condyle. This study aimed to evaluate the effects of adipose tissue-derived mesenchymal stem cells (ADMSCs), low-level laser therapy (LLLT), and grape-seed extract (GSE) on condylar growth after functio...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Galenos Publishing
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10318849/ https://www.ncbi.nlm.nih.gov/pubmed/37345991 http://dx.doi.org/10.4274/TurkJOrthod.2022.2022.92 |
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author | Eğlenen, Merve Nur Tuğlu, Mehmet İbrahim Aydemir, Işıl Güleç, Ayşegül |
author_facet | Eğlenen, Merve Nur Tuğlu, Mehmet İbrahim Aydemir, Işıl Güleç, Ayşegül |
author_sort | Eğlenen, Merve Nur |
collection | PubMed |
description | OBJECTIVE: Functional treatment of Class II malocclusion is expected to lead to adaptation in the condyle. This study aimed to evaluate the effects of adipose tissue-derived mesenchymal stem cells (ADMSCs), low-level laser therapy (LLLT), and grape-seed extract (GSE) on condylar growth after functional mandibular advancement. METHODS: Forty-five rats were randomly divided into 8 groups. Functional appliances were applied to all groups (n=6) except the control group (n=3). One group was treated with appliances only; the other six groups received various combinations of ADMSCs, LLLT, and GSE. Analyses for new osteoblasts and new bone formation, vascular endothelial growth factor, and Type II collagen were performed on condylar tissues, after an experimental period of four weeks. The quantitative data obtained from the results of the experiments were evaluated by H-score and analyzed using One-Way ANOVA by Tukey-Kramer multiple comparisons test (p≤0.05). RESULTS: Levels of all investigated parameters increased in all groups (p≤0.05). The highest increases were achieved by a combined application of functional appliance, ADMSCs, LLLT and GSE (p≤0.05). Single LLLT administrations or single GSE applications did not create a statistical difference from appliance alone (p>0.05). A positive effect of ADMSCs or LLLT on osteoblast formation, neovascularization, and Type II collagen level was apparent (p≤0.05), however, neither affected new bone formation (p>0.05). CONCLUSION: This study shows that ADMSCs with LLLT and GSE applications provide differing levels of new osteoblast and bone formation, new vascular formation, and Type II collagen formation in rat condyles after functional mandibular advancement. |
format | Online Article Text |
id | pubmed-10318849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Galenos Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-103188492023-07-05 The Condylar Effects of Mesenchymal Stem Cells, Low-Level Laser Therapy and Grape Seed Extract on Functional Mandibular Advancement of the Rat Mandible Eğlenen, Merve Nur Tuğlu, Mehmet İbrahim Aydemir, Işıl Güleç, Ayşegül Turk J Orthod Original Article OBJECTIVE: Functional treatment of Class II malocclusion is expected to lead to adaptation in the condyle. This study aimed to evaluate the effects of adipose tissue-derived mesenchymal stem cells (ADMSCs), low-level laser therapy (LLLT), and grape-seed extract (GSE) on condylar growth after functional mandibular advancement. METHODS: Forty-five rats were randomly divided into 8 groups. Functional appliances were applied to all groups (n=6) except the control group (n=3). One group was treated with appliances only; the other six groups received various combinations of ADMSCs, LLLT, and GSE. Analyses for new osteoblasts and new bone formation, vascular endothelial growth factor, and Type II collagen were performed on condylar tissues, after an experimental period of four weeks. The quantitative data obtained from the results of the experiments were evaluated by H-score and analyzed using One-Way ANOVA by Tukey-Kramer multiple comparisons test (p≤0.05). RESULTS: Levels of all investigated parameters increased in all groups (p≤0.05). The highest increases were achieved by a combined application of functional appliance, ADMSCs, LLLT and GSE (p≤0.05). Single LLLT administrations or single GSE applications did not create a statistical difference from appliance alone (p>0.05). A positive effect of ADMSCs or LLLT on osteoblast formation, neovascularization, and Type II collagen level was apparent (p≤0.05), however, neither affected new bone formation (p>0.05). CONCLUSION: This study shows that ADMSCs with LLLT and GSE applications provide differing levels of new osteoblast and bone formation, new vascular formation, and Type II collagen formation in rat condyles after functional mandibular advancement. Galenos Publishing 2023-06-22 /pmc/articles/PMC10318849/ /pubmed/37345991 http://dx.doi.org/10.4274/TurkJOrthod.2022.2022.92 Text en © Copyright 2023 by Turkish Orthodontic Society | Turkish Journal of Orthodontics, published by Galenos Publishing House. https://creativecommons.org/licenses/by-nc/4.0/Content of this journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. (https://creativecommons.org/licenses/by-nc/4.0/) |
spellingShingle | Original Article Eğlenen, Merve Nur Tuğlu, Mehmet İbrahim Aydemir, Işıl Güleç, Ayşegül The Condylar Effects of Mesenchymal Stem Cells, Low-Level Laser Therapy and Grape Seed Extract on Functional Mandibular Advancement of the Rat Mandible |
title | The Condylar Effects of Mesenchymal Stem Cells, Low-Level Laser Therapy and Grape Seed Extract on Functional Mandibular Advancement of the Rat Mandible |
title_full | The Condylar Effects of Mesenchymal Stem Cells, Low-Level Laser Therapy and Grape Seed Extract on Functional Mandibular Advancement of the Rat Mandible |
title_fullStr | The Condylar Effects of Mesenchymal Stem Cells, Low-Level Laser Therapy and Grape Seed Extract on Functional Mandibular Advancement of the Rat Mandible |
title_full_unstemmed | The Condylar Effects of Mesenchymal Stem Cells, Low-Level Laser Therapy and Grape Seed Extract on Functional Mandibular Advancement of the Rat Mandible |
title_short | The Condylar Effects of Mesenchymal Stem Cells, Low-Level Laser Therapy and Grape Seed Extract on Functional Mandibular Advancement of the Rat Mandible |
title_sort | condylar effects of mesenchymal stem cells, low-level laser therapy and grape seed extract on functional mandibular advancement of the rat mandible |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10318849/ https://www.ncbi.nlm.nih.gov/pubmed/37345991 http://dx.doi.org/10.4274/TurkJOrthod.2022.2022.92 |
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