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Choanoflagellates alongside diverse uncultured predatory protists consume the abundant open-ocean cyanobacterium Prochlorococcus

Prochlorococcus is a key member of open-ocean primary producer communities. Despite its importance, little is known about the predators that consume this cyanobacterium and make its biomass available to higher trophic levels. We identify potential predators along a gradient wherein Prochlorococcus a...

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Autores principales: Wilken, Susanne, Yung, Charmaine C. M., Poirier, Camille, Massana, Ramon, Jimenez, Valeria, Worden, Alexandra Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10318953/
https://www.ncbi.nlm.nih.gov/pubmed/37364109
http://dx.doi.org/10.1073/pnas.2302388120
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author Wilken, Susanne
Yung, Charmaine C. M.
Poirier, Camille
Massana, Ramon
Jimenez, Valeria
Worden, Alexandra Z.
author_facet Wilken, Susanne
Yung, Charmaine C. M.
Poirier, Camille
Massana, Ramon
Jimenez, Valeria
Worden, Alexandra Z.
author_sort Wilken, Susanne
collection PubMed
description Prochlorococcus is a key member of open-ocean primary producer communities. Despite its importance, little is known about the predators that consume this cyanobacterium and make its biomass available to higher trophic levels. We identify potential predators along a gradient wherein Prochlorococcus abundance increased from near detection limits (coastal California) to >200,000 cells mL(−1) (subtropical North Pacific Gyre). A replicated RNA-Stable Isotope Probing experiment involving the in situ community, and labeled Prochlorococcus as prey, revealed choanoflagellates as the most active predators of Prochlorococcus, alongside a radiolarian, chrysophytes, dictyochophytes, and specific MAST lineages. These predators were not appropriately highlighted in multiyear conventional 18S rRNA gene amplicon surveys where dinoflagellates and other taxa had highest relative amplicon abundances across the gradient. In identifying direct consumers of Prochlorococcus, we reveal food-web linkages of individual protistan taxa and resolve routes of carbon transfer from the base of marine food webs.
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spelling pubmed-103189532023-07-05 Choanoflagellates alongside diverse uncultured predatory protists consume the abundant open-ocean cyanobacterium Prochlorococcus Wilken, Susanne Yung, Charmaine C. M. Poirier, Camille Massana, Ramon Jimenez, Valeria Worden, Alexandra Z. Proc Natl Acad Sci U S A Biological Sciences Prochlorococcus is a key member of open-ocean primary producer communities. Despite its importance, little is known about the predators that consume this cyanobacterium and make its biomass available to higher trophic levels. We identify potential predators along a gradient wherein Prochlorococcus abundance increased from near detection limits (coastal California) to >200,000 cells mL(−1) (subtropical North Pacific Gyre). A replicated RNA-Stable Isotope Probing experiment involving the in situ community, and labeled Prochlorococcus as prey, revealed choanoflagellates as the most active predators of Prochlorococcus, alongside a radiolarian, chrysophytes, dictyochophytes, and specific MAST lineages. These predators were not appropriately highlighted in multiyear conventional 18S rRNA gene amplicon surveys where dinoflagellates and other taxa had highest relative amplicon abundances across the gradient. In identifying direct consumers of Prochlorococcus, we reveal food-web linkages of individual protistan taxa and resolve routes of carbon transfer from the base of marine food webs. National Academy of Sciences 2023-06-26 2023-07-04 /pmc/articles/PMC10318953/ /pubmed/37364109 http://dx.doi.org/10.1073/pnas.2302388120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biological Sciences
Wilken, Susanne
Yung, Charmaine C. M.
Poirier, Camille
Massana, Ramon
Jimenez, Valeria
Worden, Alexandra Z.
Choanoflagellates alongside diverse uncultured predatory protists consume the abundant open-ocean cyanobacterium Prochlorococcus
title Choanoflagellates alongside diverse uncultured predatory protists consume the abundant open-ocean cyanobacterium Prochlorococcus
title_full Choanoflagellates alongside diverse uncultured predatory protists consume the abundant open-ocean cyanobacterium Prochlorococcus
title_fullStr Choanoflagellates alongside diverse uncultured predatory protists consume the abundant open-ocean cyanobacterium Prochlorococcus
title_full_unstemmed Choanoflagellates alongside diverse uncultured predatory protists consume the abundant open-ocean cyanobacterium Prochlorococcus
title_short Choanoflagellates alongside diverse uncultured predatory protists consume the abundant open-ocean cyanobacterium Prochlorococcus
title_sort choanoflagellates alongside diverse uncultured predatory protists consume the abundant open-ocean cyanobacterium prochlorococcus
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10318953/
https://www.ncbi.nlm.nih.gov/pubmed/37364109
http://dx.doi.org/10.1073/pnas.2302388120
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