Cargando…

Microbial interactions and metabolisms in response to bacterial wilt and black shank pathogens in the tobacco rhizosphere

BACKGROUND: Tobacco bacterial wilt (TBW) and black shank (TBS) are responsible for substantial economic losses worldwide; however, microbial interactions and metabolisms in response to TBW and TBS pathogens in the tobacco rhizosphere remain unclear. METHODS: We explored and compared the response of...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Qianjun, Liu, Tianbo, Teng, Kai, Xiao, Zhipeng, Cai, Hailin, Wang, Yunsheng, Xiao, Yunhua, Chen, Wu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10319149/
https://www.ncbi.nlm.nih.gov/pubmed/37409299
http://dx.doi.org/10.3389/fpls.2023.1200136
_version_ 1785068184008654848
author Tang, Qianjun
Liu, Tianbo
Teng, Kai
Xiao, Zhipeng
Cai, Hailin
Wang, Yunsheng
Xiao, Yunhua
Chen, Wu
author_facet Tang, Qianjun
Liu, Tianbo
Teng, Kai
Xiao, Zhipeng
Cai, Hailin
Wang, Yunsheng
Xiao, Yunhua
Chen, Wu
author_sort Tang, Qianjun
collection PubMed
description BACKGROUND: Tobacco bacterial wilt (TBW) and black shank (TBS) are responsible for substantial economic losses worldwide; however, microbial interactions and metabolisms in response to TBW and TBS pathogens in the tobacco rhizosphere remain unclear. METHODS: We explored and compared the response of rhizosphere microbial communities to these two plant diseases with the incidences in moderate and heavy degrees by sequencing of 16S rRNA gene amplicons and bioinformatics analysis. RESULTS AND DISCUSSIONS: We found that the structure of rhizosphere soil bacterial communities was significantly (p < 0.05) changed from the incidences of TBW and TBS, which also led to decreased Shannon diversity and Pielou evenness. Compared with the healthy group (CK), the OTUs with significantly (p < 0.05) decreased relative abundances were mostly affiliated with Actinobacteria (e.g., Streptomyces and Arthrobacter) in the diseased groups, and the OTUs with significantly (p < 0.05) increased relative abundances were mainly identified as Proteobacteria and Acidobacteria. Also, molecular ecological network analysis showed that the nodes (<467) and links (<641) were decreased in the diseased groups compared with the control group (572; 1056), suggesting that both TBW and TBS weakened bacterial interactions. In addition, the predictive functional analysis indicated that the relative abundance of genes related to the biosynthesis of antibiotics (e.g., ansamycins and streptomycin) was significantly (p < 0.05) decreased due to incidences of TBW and TBS, and antimicrobial tests showed that some Actinobacteria strains (e.g., Streptomyces) and their secreted antibiotics (e.g., streptomycin) could effectively inhibit the growth of these two pathogens.
format Online
Article
Text
id pubmed-10319149
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-103191492023-07-05 Microbial interactions and metabolisms in response to bacterial wilt and black shank pathogens in the tobacco rhizosphere Tang, Qianjun Liu, Tianbo Teng, Kai Xiao, Zhipeng Cai, Hailin Wang, Yunsheng Xiao, Yunhua Chen, Wu Front Plant Sci Plant Science BACKGROUND: Tobacco bacterial wilt (TBW) and black shank (TBS) are responsible for substantial economic losses worldwide; however, microbial interactions and metabolisms in response to TBW and TBS pathogens in the tobacco rhizosphere remain unclear. METHODS: We explored and compared the response of rhizosphere microbial communities to these two plant diseases with the incidences in moderate and heavy degrees by sequencing of 16S rRNA gene amplicons and bioinformatics analysis. RESULTS AND DISCUSSIONS: We found that the structure of rhizosphere soil bacterial communities was significantly (p < 0.05) changed from the incidences of TBW and TBS, which also led to decreased Shannon diversity and Pielou evenness. Compared with the healthy group (CK), the OTUs with significantly (p < 0.05) decreased relative abundances were mostly affiliated with Actinobacteria (e.g., Streptomyces and Arthrobacter) in the diseased groups, and the OTUs with significantly (p < 0.05) increased relative abundances were mainly identified as Proteobacteria and Acidobacteria. Also, molecular ecological network analysis showed that the nodes (<467) and links (<641) were decreased in the diseased groups compared with the control group (572; 1056), suggesting that both TBW and TBS weakened bacterial interactions. In addition, the predictive functional analysis indicated that the relative abundance of genes related to the biosynthesis of antibiotics (e.g., ansamycins and streptomycin) was significantly (p < 0.05) decreased due to incidences of TBW and TBS, and antimicrobial tests showed that some Actinobacteria strains (e.g., Streptomyces) and their secreted antibiotics (e.g., streptomycin) could effectively inhibit the growth of these two pathogens. Frontiers Media S.A. 2023-06-20 /pmc/articles/PMC10319149/ /pubmed/37409299 http://dx.doi.org/10.3389/fpls.2023.1200136 Text en Copyright © 2023 Tang, Liu, Teng, Xiao, Cai, Wang, Xiao and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Tang, Qianjun
Liu, Tianbo
Teng, Kai
Xiao, Zhipeng
Cai, Hailin
Wang, Yunsheng
Xiao, Yunhua
Chen, Wu
Microbial interactions and metabolisms in response to bacterial wilt and black shank pathogens in the tobacco rhizosphere
title Microbial interactions and metabolisms in response to bacterial wilt and black shank pathogens in the tobacco rhizosphere
title_full Microbial interactions and metabolisms in response to bacterial wilt and black shank pathogens in the tobacco rhizosphere
title_fullStr Microbial interactions and metabolisms in response to bacterial wilt and black shank pathogens in the tobacco rhizosphere
title_full_unstemmed Microbial interactions and metabolisms in response to bacterial wilt and black shank pathogens in the tobacco rhizosphere
title_short Microbial interactions and metabolisms in response to bacterial wilt and black shank pathogens in the tobacco rhizosphere
title_sort microbial interactions and metabolisms in response to bacterial wilt and black shank pathogens in the tobacco rhizosphere
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10319149/
https://www.ncbi.nlm.nih.gov/pubmed/37409299
http://dx.doi.org/10.3389/fpls.2023.1200136
work_keys_str_mv AT tangqianjun microbialinteractionsandmetabolismsinresponsetobacterialwiltandblackshankpathogensinthetobaccorhizosphere
AT liutianbo microbialinteractionsandmetabolismsinresponsetobacterialwiltandblackshankpathogensinthetobaccorhizosphere
AT tengkai microbialinteractionsandmetabolismsinresponsetobacterialwiltandblackshankpathogensinthetobaccorhizosphere
AT xiaozhipeng microbialinteractionsandmetabolismsinresponsetobacterialwiltandblackshankpathogensinthetobaccorhizosphere
AT caihailin microbialinteractionsandmetabolismsinresponsetobacterialwiltandblackshankpathogensinthetobaccorhizosphere
AT wangyunsheng microbialinteractionsandmetabolismsinresponsetobacterialwiltandblackshankpathogensinthetobaccorhizosphere
AT xiaoyunhua microbialinteractionsandmetabolismsinresponsetobacterialwiltandblackshankpathogensinthetobaccorhizosphere
AT chenwu microbialinteractionsandmetabolismsinresponsetobacterialwiltandblackshankpathogensinthetobaccorhizosphere