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Transcriptomic and metabolomic analyses reveal the potential mechanism of waterlogging resistance in cotton (Gossypium hirsutum L.)

INTRODUCTION: Cotton (Gossypium hirsutum L.) is susceptible to long-term waterlogging stress; however, genomic information of cotton response mechanisms toward long days of waterlogging is quite elusive. METHODS: Here, we combined the transcriptome and metabolome expression level changes in cotton r...

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Autores principales: Owusu, Anane Gideon, Lv, Yin-Ping, Liu, Man, Wu, Yong, Li, Cheng-Lin, Guo, Ning, Li, Da-Hui, Gao, Jun-Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10319419/
https://www.ncbi.nlm.nih.gov/pubmed/37409297
http://dx.doi.org/10.3389/fpls.2023.1088537
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author Owusu, Anane Gideon
Lv, Yin-Ping
Liu, Man
Wu, Yong
Li, Cheng-Lin
Guo, Ning
Li, Da-Hui
Gao, Jun-Shan
author_facet Owusu, Anane Gideon
Lv, Yin-Ping
Liu, Man
Wu, Yong
Li, Cheng-Lin
Guo, Ning
Li, Da-Hui
Gao, Jun-Shan
author_sort Owusu, Anane Gideon
collection PubMed
description INTRODUCTION: Cotton (Gossypium hirsutum L.) is susceptible to long-term waterlogging stress; however, genomic information of cotton response mechanisms toward long days of waterlogging is quite elusive. METHODS: Here, we combined the transcriptome and metabolome expression level changes in cotton roots after 10 and 20 days of waterlogging stress treatment pertaining to potential resistance mechanisms in two cotton genotypes. RESULTS AND DISCUSSION: Numerous adventitious roots and hypertrophic lenticels were induced in CJ1831056 and CJ1831072. Transcriptome analysis revealed 101,599 differentially expressed genes in cotton roots with higher gene expression after 20 days of stress. Reactive oxygen species (ROS) generating genes, antioxidant enzyme genes, and transcription factor genes (AP2, MYB, WRKY, and bZIP) were highly responsive to waterlogging stress among the two genotypes. Metabolomics results showed higher expressions of stress-resistant metabolites sinapyl alcohol, L-glutamic acid, galactaric acid, glucose 1-phosphate, L-valine, L-asparagine, and melibiose in CJ1831056 than CJ1831072. Differentially expressed metabolites (adenosine, galactaric acid, sinapyl alcohol, L-valine, L-asparagine, and melibiose) significantly correlated with the differentially expressed PRX52, PER1, PER64, and BGLU11 transcripts. This investigation reveals genes for targeted genetic engineering to improve waterlogging stress resistance to enhance abiotic stress regulatory mechanisms in cotton at the transcript and metabolic levels of study.
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spelling pubmed-103194192023-07-05 Transcriptomic and metabolomic analyses reveal the potential mechanism of waterlogging resistance in cotton (Gossypium hirsutum L.) Owusu, Anane Gideon Lv, Yin-Ping Liu, Man Wu, Yong Li, Cheng-Lin Guo, Ning Li, Da-Hui Gao, Jun-Shan Front Plant Sci Plant Science INTRODUCTION: Cotton (Gossypium hirsutum L.) is susceptible to long-term waterlogging stress; however, genomic information of cotton response mechanisms toward long days of waterlogging is quite elusive. METHODS: Here, we combined the transcriptome and metabolome expression level changes in cotton roots after 10 and 20 days of waterlogging stress treatment pertaining to potential resistance mechanisms in two cotton genotypes. RESULTS AND DISCUSSION: Numerous adventitious roots and hypertrophic lenticels were induced in CJ1831056 and CJ1831072. Transcriptome analysis revealed 101,599 differentially expressed genes in cotton roots with higher gene expression after 20 days of stress. Reactive oxygen species (ROS) generating genes, antioxidant enzyme genes, and transcription factor genes (AP2, MYB, WRKY, and bZIP) were highly responsive to waterlogging stress among the two genotypes. Metabolomics results showed higher expressions of stress-resistant metabolites sinapyl alcohol, L-glutamic acid, galactaric acid, glucose 1-phosphate, L-valine, L-asparagine, and melibiose in CJ1831056 than CJ1831072. Differentially expressed metabolites (adenosine, galactaric acid, sinapyl alcohol, L-valine, L-asparagine, and melibiose) significantly correlated with the differentially expressed PRX52, PER1, PER64, and BGLU11 transcripts. This investigation reveals genes for targeted genetic engineering to improve waterlogging stress resistance to enhance abiotic stress regulatory mechanisms in cotton at the transcript and metabolic levels of study. Frontiers Media S.A. 2023-06-12 /pmc/articles/PMC10319419/ /pubmed/37409297 http://dx.doi.org/10.3389/fpls.2023.1088537 Text en Copyright © 2023 Owusu, Lv, Liu, Wu, Li, Guo, Li and Gao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Owusu, Anane Gideon
Lv, Yin-Ping
Liu, Man
Wu, Yong
Li, Cheng-Lin
Guo, Ning
Li, Da-Hui
Gao, Jun-Shan
Transcriptomic and metabolomic analyses reveal the potential mechanism of waterlogging resistance in cotton (Gossypium hirsutum L.)
title Transcriptomic and metabolomic analyses reveal the potential mechanism of waterlogging resistance in cotton (Gossypium hirsutum L.)
title_full Transcriptomic and metabolomic analyses reveal the potential mechanism of waterlogging resistance in cotton (Gossypium hirsutum L.)
title_fullStr Transcriptomic and metabolomic analyses reveal the potential mechanism of waterlogging resistance in cotton (Gossypium hirsutum L.)
title_full_unstemmed Transcriptomic and metabolomic analyses reveal the potential mechanism of waterlogging resistance in cotton (Gossypium hirsutum L.)
title_short Transcriptomic and metabolomic analyses reveal the potential mechanism of waterlogging resistance in cotton (Gossypium hirsutum L.)
title_sort transcriptomic and metabolomic analyses reveal the potential mechanism of waterlogging resistance in cotton (gossypium hirsutum l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10319419/
https://www.ncbi.nlm.nih.gov/pubmed/37409297
http://dx.doi.org/10.3389/fpls.2023.1088537
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