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High-speed scanless entire bandwidth mid-infrared chemical imaging
Mid-infrared spectroscopy probes molecular vibrations to identify chemical species and functional groups. Therefore, mid-infrared hyperspectral imaging is one of the most powerful and promising candidates for chemical imaging using optical methods. Yet high-speed and entire bandwidth mid-infrared hy...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10319884/ https://www.ncbi.nlm.nih.gov/pubmed/37402722 http://dx.doi.org/10.1038/s41467-023-39628-6 |
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author | Zhao, Yue Kusama, Shota Furutani, Yuji Huang, Wei-Hong Luo, Chih-Wei Fuji, Takao |
author_facet | Zhao, Yue Kusama, Shota Furutani, Yuji Huang, Wei-Hong Luo, Chih-Wei Fuji, Takao |
author_sort | Zhao, Yue |
collection | PubMed |
description | Mid-infrared spectroscopy probes molecular vibrations to identify chemical species and functional groups. Therefore, mid-infrared hyperspectral imaging is one of the most powerful and promising candidates for chemical imaging using optical methods. Yet high-speed and entire bandwidth mid-infrared hyperspectral imaging has not been realized. Here we report a mid-infrared hyperspectral chemical imaging technique that uses chirped pulse upconversion of sub-cycle pulses at the image plane. This technique offers a lateral resolution of 15 µm, and the field of view is adjustable between 800 µm × 600 µm to 12 mm × 9 mm. The hyperspectral imaging produces a 640 × 480 pixel image in 8 s, which covers a spectral range of 640–3015 cm(–1), comprising 1069 wavelength points and offering a wavenumber resolution of 2.6–3.7 cm(–1). For discrete frequency mid-infrared imaging, the measurement speed reaches a frame rate of 5 kHz, the repetition rate of the laser. As a demonstration, we effectively identified and mapped different components in a microfluidic device, plant cell, and mouse embryo section. The great capacity and latent force of this technique in chemical imaging promise to be applied to many fields such as chemical analysis, biology, and medicine. |
format | Online Article Text |
id | pubmed-10319884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-103198842023-07-06 High-speed scanless entire bandwidth mid-infrared chemical imaging Zhao, Yue Kusama, Shota Furutani, Yuji Huang, Wei-Hong Luo, Chih-Wei Fuji, Takao Nat Commun Article Mid-infrared spectroscopy probes molecular vibrations to identify chemical species and functional groups. Therefore, mid-infrared hyperspectral imaging is one of the most powerful and promising candidates for chemical imaging using optical methods. Yet high-speed and entire bandwidth mid-infrared hyperspectral imaging has not been realized. Here we report a mid-infrared hyperspectral chemical imaging technique that uses chirped pulse upconversion of sub-cycle pulses at the image plane. This technique offers a lateral resolution of 15 µm, and the field of view is adjustable between 800 µm × 600 µm to 12 mm × 9 mm. The hyperspectral imaging produces a 640 × 480 pixel image in 8 s, which covers a spectral range of 640–3015 cm(–1), comprising 1069 wavelength points and offering a wavenumber resolution of 2.6–3.7 cm(–1). For discrete frequency mid-infrared imaging, the measurement speed reaches a frame rate of 5 kHz, the repetition rate of the laser. As a demonstration, we effectively identified and mapped different components in a microfluidic device, plant cell, and mouse embryo section. The great capacity and latent force of this technique in chemical imaging promise to be applied to many fields such as chemical analysis, biology, and medicine. Nature Publishing Group UK 2023-07-04 /pmc/articles/PMC10319884/ /pubmed/37402722 http://dx.doi.org/10.1038/s41467-023-39628-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhao, Yue Kusama, Shota Furutani, Yuji Huang, Wei-Hong Luo, Chih-Wei Fuji, Takao High-speed scanless entire bandwidth mid-infrared chemical imaging |
title | High-speed scanless entire bandwidth mid-infrared chemical imaging |
title_full | High-speed scanless entire bandwidth mid-infrared chemical imaging |
title_fullStr | High-speed scanless entire bandwidth mid-infrared chemical imaging |
title_full_unstemmed | High-speed scanless entire bandwidth mid-infrared chemical imaging |
title_short | High-speed scanless entire bandwidth mid-infrared chemical imaging |
title_sort | high-speed scanless entire bandwidth mid-infrared chemical imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10319884/ https://www.ncbi.nlm.nih.gov/pubmed/37402722 http://dx.doi.org/10.1038/s41467-023-39628-6 |
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