Protocol for high-throughput electron tomography exemplified on centrioles in primary human plasma cells

Electron microscopy is the gold standard to characterize cellular ultrastructure. However, production of significant morphometrical data is highly limited by acquisition time. Here, we describe a semi-automated high-throughput strategy using single-axis serial section electron tomography to investig...

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Detalles Bibliográficos
Autores principales: Köhrer, Sebastian, Dittrich, Tobias, Schorb, Martin, Haberbosch, Isabella, Schwab, Yannick, Krämer, Alwin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320260/
https://www.ncbi.nlm.nih.gov/pubmed/37354457
http://dx.doi.org/10.1016/j.xpro.2023.102373

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320260/
https://www.ncbi.nlm.nih.gov/pubmed/37354457
http://dx.doi.org/10.1016/j.xpro.2023.102373

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