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Protocol for high-throughput electron tomography exemplified on centrioles in primary human plasma cells
Electron microscopy is the gold standard to characterize cellular ultrastructure. However, production of significant morphometrical data is highly limited by acquisition time. Here, we describe a semi-automated high-throughput strategy using single-axis serial section electron tomography to investig...
Autores principales: | Köhrer, Sebastian, Dittrich, Tobias, Schorb, Martin, Haberbosch, Isabella, Schwab, Yannick, Krämer, Alwin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320260/ https://www.ncbi.nlm.nih.gov/pubmed/37354457 http://dx.doi.org/10.1016/j.xpro.2023.102373 |
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