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Protocol for extracting live blastoderm cells from embryos of annual killifish

The implementation of in vitro approaches using undifferentiated embryonic cells from annual killifish to complement existing in vivo developmental studies has been hindered by a lack of efficient isolation techniques. Here, we present a protocol to isolate annual killifish blastoderm cells, at the...

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Detalles Bibliográficos
Autores principales: Vásquez-Sepúlveda, Sebastian, Guerrero, Nestor, Lemus, Carmen Gloria, Meynard, Margarita, Cerda, Mauricio, Concha, Miguel Luis, Bertocchi, Cristina, Ravasio, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320274/
https://www.ncbi.nlm.nih.gov/pubmed/37352104
http://dx.doi.org/10.1016/j.xpro.2023.102344

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