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Whole genome sequencing data of Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia
Candida krusei is a Candida non-albicans species with a high prevalence, which causes candidaemia. Current treatment guidelines include fluconazole as a primary therapeutic option for the treatment of these infections; however, it is only a fungistatic against Candida spp., and both inherent and acq...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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F1000 Research Limited
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320322/ https://www.ncbi.nlm.nih.gov/pubmed/37416067 http://dx.doi.org/10.12688/f1000research.121402.2 |
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author | Fadilah, Fadilah Adawiyah, Robiatul Wahyuningsih, Retno Rozaliyani, Anna Fatril, Ayu Eka Paramita, Rafika Indah Erlina, Linda Istiadi, Khaerunissa Anbar |
author_facet | Fadilah, Fadilah Adawiyah, Robiatul Wahyuningsih, Retno Rozaliyani, Anna Fatril, Ayu Eka Paramita, Rafika Indah Erlina, Linda Istiadi, Khaerunissa Anbar |
author_sort | Fadilah, Fadilah |
collection | PubMed |
description | Candida krusei is a Candida non-albicans species with a high prevalence, which causes candidaemia. Current treatment guidelines include fluconazole as a primary therapeutic option for the treatment of these infections; however, it is only a fungistatic against Candida spp., and both inherent and acquired resistance to fluconazole have been reported. C. krusei species is also reported as the only Candida sp. which has an intrinsic resistance factor to fluconazole. Therefore, in dealing with antifungal resistance, it is necessary to develop new antifungal agents that are efficient in the treatment of fungal infections, especially those caused by C. krusei. The purpose of this study was to investigate the genome of clinical C. krusei isolates and correlate the resistant phenotypes with mutations in resistance genes. A total of 16 samples of C. krusei from clinical samples from hospitals in Jakarta were used in the experiment. All colonies were extracted using the QIAamp DNA Mini Kit. The library was prepared using the Illumina DNA Prep Kit. The sequencing process was carried out on the Illumina MiSeq Platform using a 2x301 paired-end configuration. FASTQ raw files are available under the BioProject Accession Number PRJNA819536 and Sequence Read Archive Accession Numbers SRR18739949 and SRR18739964. |
format | Online Article Text |
id | pubmed-10320322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | F1000 Research Limited |
record_format | MEDLINE/PubMed |
spelling | pubmed-103203222023-07-06 Whole genome sequencing data of Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia Fadilah, Fadilah Adawiyah, Robiatul Wahyuningsih, Retno Rozaliyani, Anna Fatril, Ayu Eka Paramita, Rafika Indah Erlina, Linda Istiadi, Khaerunissa Anbar F1000Res Genome Note Candida krusei is a Candida non-albicans species with a high prevalence, which causes candidaemia. Current treatment guidelines include fluconazole as a primary therapeutic option for the treatment of these infections; however, it is only a fungistatic against Candida spp., and both inherent and acquired resistance to fluconazole have been reported. C. krusei species is also reported as the only Candida sp. which has an intrinsic resistance factor to fluconazole. Therefore, in dealing with antifungal resistance, it is necessary to develop new antifungal agents that are efficient in the treatment of fungal infections, especially those caused by C. krusei. The purpose of this study was to investigate the genome of clinical C. krusei isolates and correlate the resistant phenotypes with mutations in resistance genes. A total of 16 samples of C. krusei from clinical samples from hospitals in Jakarta were used in the experiment. All colonies were extracted using the QIAamp DNA Mini Kit. The library was prepared using the Illumina DNA Prep Kit. The sequencing process was carried out on the Illumina MiSeq Platform using a 2x301 paired-end configuration. FASTQ raw files are available under the BioProject Accession Number PRJNA819536 and Sequence Read Archive Accession Numbers SRR18739949 and SRR18739964. F1000 Research Limited 2023-03-29 /pmc/articles/PMC10320322/ /pubmed/37416067 http://dx.doi.org/10.12688/f1000research.121402.2 Text en Copyright: © 2023 Fadilah F et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Genome Note Fadilah, Fadilah Adawiyah, Robiatul Wahyuningsih, Retno Rozaliyani, Anna Fatril, Ayu Eka Paramita, Rafika Indah Erlina, Linda Istiadi, Khaerunissa Anbar Whole genome sequencing data of Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia |
title | Whole genome sequencing data of
Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia |
title_full | Whole genome sequencing data of
Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia |
title_fullStr | Whole genome sequencing data of
Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia |
title_full_unstemmed | Whole genome sequencing data of
Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia |
title_short | Whole genome sequencing data of
Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia |
title_sort | whole genome sequencing data of
candida krusei, the pathogen causing candidaemia, from department of parasitology culture collection, faculty of medicine universitas indonesia |
topic | Genome Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320322/ https://www.ncbi.nlm.nih.gov/pubmed/37416067 http://dx.doi.org/10.12688/f1000research.121402.2 |
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