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Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition

INTRODUCTION: Dietary medicinal plants are among the most sought-after topics in alternative medicine today due to their preventive and healing properties against many diseases. AIM: This study aimed to extract and determine the polyphenols from indigenous plants extracts, i.e., Mentha longifolia, M...

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Autores principales: Mueed, Abdul, Shibli, Sahar, Al-Quwaie, Diana A., Ashkan, Mada F., Alharbi, Mona, Alanazi, Humidah, Binothman, Najat, Aljadani, Majidah, Majrashi, Kamlah Ali, Huwaikem, Mashael, Abourehab, Mohammed A. S., Korma, Sameh A., El-Saadony, Mohamed T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320526/
https://www.ncbi.nlm.nih.gov/pubmed/37415912
http://dx.doi.org/10.3389/fnut.2023.1125106
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author Mueed, Abdul
Shibli, Sahar
Al-Quwaie, Diana A.
Ashkan, Mada F.
Alharbi, Mona
Alanazi, Humidah
Binothman, Najat
Aljadani, Majidah
Majrashi, Kamlah Ali
Huwaikem, Mashael
Abourehab, Mohammed A. S.
Korma, Sameh A.
El-Saadony, Mohamed T.
author_facet Mueed, Abdul
Shibli, Sahar
Al-Quwaie, Diana A.
Ashkan, Mada F.
Alharbi, Mona
Alanazi, Humidah
Binothman, Najat
Aljadani, Majidah
Majrashi, Kamlah Ali
Huwaikem, Mashael
Abourehab, Mohammed A. S.
Korma, Sameh A.
El-Saadony, Mohamed T.
author_sort Mueed, Abdul
collection PubMed
description INTRODUCTION: Dietary medicinal plants are among the most sought-after topics in alternative medicine today due to their preventive and healing properties against many diseases. AIM: This study aimed to extract and determine the polyphenols from indigenous plants extracts, i.e., Mentha longifolia, M. arvensis, Tinospora cordifolia, Cymbopogon citratus, Foeniculum vulgare, Cassia absus, Camellia sinensis, Trachyspermum ammi, C. sinensis and M. arvensis, then evaluate the antioxidant, cytotoxicity, and antimicrobial properties, besides enzyme inhibition of isolated polyphenols. METHODS: The antioxidant activity was evaluated by DPPH, Superoxide radical, Hydroxyl radical (OH(.)), and Nitric oxide (NO(.)) scavenging activity; the antidiabetic activity was evaluated by enzymatic methods, and anticancer activity using MTT assay, while the antibacterial activity. RESULTS: The results showed that tested medicinal plants’ polyphenolic extracts (MPPE) exhibited the most significant antioxidant activity in DPPH, hydroxyl, nitric oxide, and superoxide radical scavenging methods because of the considerable amounts of total polyphenol and flavonoid contents. UHPLC profile showed twenty-five polyphenol complexes in eight medicinal plant extracts, categorized into phenolic acids, flavonoids, and alkaloids. The main polyphenol was 3-Feroylquinic acid (1,302 mg/L), also found in M. longifolia, C. absus, and C. sinensis, has a higher phenolic content, i.e., rosmarinic acid, vanillic acid, chlorogenic acid, p-coumaric acid, ferulic acid, gallic acid, catechin, luteolin, 7-O-neohesperideside, quercetin 3,7-O-glucoside, hesperidin, rutin, quercetin, and caffeine in the range of (560–780 mg/L). At the same time, other compounds are of medium content (99–312 mg/L). The phenolics in C. sinensis were 20–116% more abundant than those in M. longifolia, C. absus, and other medicinal plants. While T. cordifolia is rich in alkaloids, T. ammi has a lower content. The MTT assay against Caco-2 cells showed that polyphenolic extracts of T. ammi and C. citratus had maximum cytotoxicity. While M. arvensis, C. sinensis, and F. vulgare extracts showed significant enzyme inhibition activity, C. sinensis showed minor inhibition activity against α-amylase. Furthermore, F. vulgare and C. sinensis polyphenolic extracts showed considerable antibacterial activity against S. aureus, B. cereus, E. coli, and S. enterica. DISCUSSION: The principal component analysis demonstrated clear separation among medicinal plants’ extracts based on their functional properties. These findings prove the therapeutic effectiveness of indigenous plants and highlight their importance as natural reserves of phytogenic compounds with untapped potential that needs to be discovered through advanced analytical methods.
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spelling pubmed-103205262023-07-06 Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition Mueed, Abdul Shibli, Sahar Al-Quwaie, Diana A. Ashkan, Mada F. Alharbi, Mona Alanazi, Humidah Binothman, Najat Aljadani, Majidah Majrashi, Kamlah Ali Huwaikem, Mashael Abourehab, Mohammed A. S. Korma, Sameh A. El-Saadony, Mohamed T. Front Nutr Nutrition INTRODUCTION: Dietary medicinal plants are among the most sought-after topics in alternative medicine today due to their preventive and healing properties against many diseases. AIM: This study aimed to extract and determine the polyphenols from indigenous plants extracts, i.e., Mentha longifolia, M. arvensis, Tinospora cordifolia, Cymbopogon citratus, Foeniculum vulgare, Cassia absus, Camellia sinensis, Trachyspermum ammi, C. sinensis and M. arvensis, then evaluate the antioxidant, cytotoxicity, and antimicrobial properties, besides enzyme inhibition of isolated polyphenols. METHODS: The antioxidant activity was evaluated by DPPH, Superoxide radical, Hydroxyl radical (OH(.)), and Nitric oxide (NO(.)) scavenging activity; the antidiabetic activity was evaluated by enzymatic methods, and anticancer activity using MTT assay, while the antibacterial activity. RESULTS: The results showed that tested medicinal plants’ polyphenolic extracts (MPPE) exhibited the most significant antioxidant activity in DPPH, hydroxyl, nitric oxide, and superoxide radical scavenging methods because of the considerable amounts of total polyphenol and flavonoid contents. UHPLC profile showed twenty-five polyphenol complexes in eight medicinal plant extracts, categorized into phenolic acids, flavonoids, and alkaloids. The main polyphenol was 3-Feroylquinic acid (1,302 mg/L), also found in M. longifolia, C. absus, and C. sinensis, has a higher phenolic content, i.e., rosmarinic acid, vanillic acid, chlorogenic acid, p-coumaric acid, ferulic acid, gallic acid, catechin, luteolin, 7-O-neohesperideside, quercetin 3,7-O-glucoside, hesperidin, rutin, quercetin, and caffeine in the range of (560–780 mg/L). At the same time, other compounds are of medium content (99–312 mg/L). The phenolics in C. sinensis were 20–116% more abundant than those in M. longifolia, C. absus, and other medicinal plants. While T. cordifolia is rich in alkaloids, T. ammi has a lower content. The MTT assay against Caco-2 cells showed that polyphenolic extracts of T. ammi and C. citratus had maximum cytotoxicity. While M. arvensis, C. sinensis, and F. vulgare extracts showed significant enzyme inhibition activity, C. sinensis showed minor inhibition activity against α-amylase. Furthermore, F. vulgare and C. sinensis polyphenolic extracts showed considerable antibacterial activity against S. aureus, B. cereus, E. coli, and S. enterica. DISCUSSION: The principal component analysis demonstrated clear separation among medicinal plants’ extracts based on their functional properties. These findings prove the therapeutic effectiveness of indigenous plants and highlight their importance as natural reserves of phytogenic compounds with untapped potential that needs to be discovered through advanced analytical methods. Frontiers Media S.A. 2023-06-21 /pmc/articles/PMC10320526/ /pubmed/37415912 http://dx.doi.org/10.3389/fnut.2023.1125106 Text en Copyright © 2023 Mueed, Shibli, Al-Quwaie, Ashkan, Alharbi, Alanazi, Binothman, Aljadani, Majrashi, Huwaikem, Abourehab, Korma and El-Saadony. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Mueed, Abdul
Shibli, Sahar
Al-Quwaie, Diana A.
Ashkan, Mada F.
Alharbi, Mona
Alanazi, Humidah
Binothman, Najat
Aljadani, Majidah
Majrashi, Kamlah Ali
Huwaikem, Mashael
Abourehab, Mohammed A. S.
Korma, Sameh A.
El-Saadony, Mohamed T.
Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition
title Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition
title_full Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition
title_fullStr Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition
title_full_unstemmed Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition
title_short Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition
title_sort extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320526/
https://www.ncbi.nlm.nih.gov/pubmed/37415912
http://dx.doi.org/10.3389/fnut.2023.1125106
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