Comparison of three DNA extraction methods for recovery of microbial DNA from Arctic permafrost

Permafrost soils, which contain one of Earth’s largest terrestrial carbon stocks, are vulnerable to thaw and microbial decomposition, exacerbating climate change. Advancements in sequencing technologies have facilitated the identification and functional profiling of microbial communities in permafro...

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Detalles Bibliográficos
Autores principales: Feng, Sarah, DeKlotz, Marla, Taş, Neslihan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
Commodity Validation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320573/
https://www.ncbi.nlm.nih.gov/pubmed/37416892
http://dx.doi.org/10.17912/micropub.biology.000834
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author Feng, Sarah
DeKlotz, Marla
Taş, Neslihan
author_facet Feng, Sarah
DeKlotz, Marla
Taş, Neslihan
author_sort Feng, Sarah
collection PubMed
description Permafrost soils, which contain one of Earth’s largest terrestrial carbon stocks, are vulnerable to thaw and microbial decomposition, exacerbating climate change. Advancements in sequencing technologies have facilitated the identification and functional profiling of microbial communities in permafrost, but DNA extraction from these soils is challenging due to their high microbial diversity and low biomass. This study assessed the effectiveness of the DNeasy PowerSoil Pro kit in extracting DNA from permafrost samples and found that it produced significantly different results than the discontinued DNeasy PowerSoil kit. The study highlights the importance of consistent DNA extraction methods in permafrost studies.
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spelling pubmed-103205732023-07-06 Comparison of three DNA extraction methods for recovery of microbial DNA from Arctic permafrost Feng, Sarah DeKlotz, Marla Taş, Neslihan MicroPubl Biol Commodity Validation Permafrost soils, which contain one of Earth’s largest terrestrial carbon stocks, are vulnerable to thaw and microbial decomposition, exacerbating climate change. Advancements in sequencing technologies have facilitated the identification and functional profiling of microbial communities in permafrost, but DNA extraction from these soils is challenging due to their high microbial diversity and low biomass. This study assessed the effectiveness of the DNeasy PowerSoil Pro kit in extracting DNA from permafrost samples and found that it produced significantly different results than the discontinued DNeasy PowerSoil kit. The study highlights the importance of consistent DNA extraction methods in permafrost studies. Caltech Library 2023-06-21 /pmc/articles/PMC10320573/ /pubmed/37416892 http://dx.doi.org/10.17912/micropub.biology.000834 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Commodity Validation
Feng, Sarah
DeKlotz, Marla
Taş, Neslihan
Comparison of three DNA extraction methods for recovery of microbial DNA from Arctic permafrost
title Comparison of three DNA extraction methods for recovery of microbial DNA from Arctic permafrost
title_full Comparison of three DNA extraction methods for recovery of microbial DNA from Arctic permafrost
title_fullStr Comparison of three DNA extraction methods for recovery of microbial DNA from Arctic permafrost
title_full_unstemmed Comparison of three DNA extraction methods for recovery of microbial DNA from Arctic permafrost
title_short Comparison of three DNA extraction methods for recovery of microbial DNA from Arctic permafrost
title_sort comparison of three dna extraction methods for recovery of microbial dna from arctic permafrost
topic Commodity Validation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320573/
https://www.ncbi.nlm.nih.gov/pubmed/37416892
http://dx.doi.org/10.17912/micropub.biology.000834
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