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Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair

Femoral shaft fracture is a common bone trauma in dogs. The limitation of mesenchymal stem cells in bone defect applications is that the cell suspension cannot be fixed to the bone defect site. In the study, our objective was to substantiate the combined application of canine bone marrow mesenchymal...

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Autores principales: Ma, Zihang, Guo, Xiaoying, Zhang, Jun, Jiang, Qifeng, Liang, Wuying, Meng, Wenxin, Chen, Shuaijiang, Zhu, Yufan, Ye, Cundong, Jia, Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320857/
https://www.ncbi.nlm.nih.gov/pubmed/37415965
http://dx.doi.org/10.3389/fvets.2023.1162407
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author Ma, Zihang
Guo, Xiaoying
Zhang, Jun
Jiang, Qifeng
Liang, Wuying
Meng, Wenxin
Chen, Shuaijiang
Zhu, Yufan
Ye, Cundong
Jia, Kun
author_facet Ma, Zihang
Guo, Xiaoying
Zhang, Jun
Jiang, Qifeng
Liang, Wuying
Meng, Wenxin
Chen, Shuaijiang
Zhu, Yufan
Ye, Cundong
Jia, Kun
author_sort Ma, Zihang
collection PubMed
description Femoral shaft fracture is a common bone trauma in dogs. The limitation of mesenchymal stem cells in bone defect applications is that the cell suspension cannot be fixed to the bone defect site. In the study, our objective was to substantiate the combined application of canine bone marrow mesenchymal stem cells (cBMSCs) and gelatin-nano-hydroxyapatite (Gel-nHAP) and evaluate its therapeutic effect on bone defect diseases in dogs. Experiments were performed to evaluate the following: (1) the porosity of Gel-nHAP; (2) the adhesion of cBMSCs to Gel-nHAP; and (3) the effect of Gel-nHAP on cBMSC proliferation. The efficacy and safety of the combination of cBMSC and Gel-nHAP in the repair of femoral shaft defects were evaluated in animal experiments. The results showed that Gel-nHAP supported the attachment of cBMSCs and exhibited good biocompatibility. In the animal bone defect repair experiment, significant cortical bone growth was observed in the Gel-nHAP group at week 8 (p < 0.05) and in the cBMSCs-Gel-nHAP group at week 4 (p < 0.01). We demonstrated that Gel-nHAP could promote the repair of bone defects, and the effect of cBMSC-Gel-nHAP on the repair of bone defects was profound.
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spelling pubmed-103208572023-07-06 Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair Ma, Zihang Guo, Xiaoying Zhang, Jun Jiang, Qifeng Liang, Wuying Meng, Wenxin Chen, Shuaijiang Zhu, Yufan Ye, Cundong Jia, Kun Front Vet Sci Veterinary Science Femoral shaft fracture is a common bone trauma in dogs. The limitation of mesenchymal stem cells in bone defect applications is that the cell suspension cannot be fixed to the bone defect site. In the study, our objective was to substantiate the combined application of canine bone marrow mesenchymal stem cells (cBMSCs) and gelatin-nano-hydroxyapatite (Gel-nHAP) and evaluate its therapeutic effect on bone defect diseases in dogs. Experiments were performed to evaluate the following: (1) the porosity of Gel-nHAP; (2) the adhesion of cBMSCs to Gel-nHAP; and (3) the effect of Gel-nHAP on cBMSC proliferation. The efficacy and safety of the combination of cBMSC and Gel-nHAP in the repair of femoral shaft defects were evaluated in animal experiments. The results showed that Gel-nHAP supported the attachment of cBMSCs and exhibited good biocompatibility. In the animal bone defect repair experiment, significant cortical bone growth was observed in the Gel-nHAP group at week 8 (p < 0.05) and in the cBMSCs-Gel-nHAP group at week 4 (p < 0.01). We demonstrated that Gel-nHAP could promote the repair of bone defects, and the effect of cBMSC-Gel-nHAP on the repair of bone defects was profound. Frontiers Media S.A. 2023-06-21 /pmc/articles/PMC10320857/ /pubmed/37415965 http://dx.doi.org/10.3389/fvets.2023.1162407 Text en Copyright © 2023 Ma, Guo, Zhang, Jiang, Liang, Meng, Chen, Zhu, Ye and Jia. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Ma, Zihang
Guo, Xiaoying
Zhang, Jun
Jiang, Qifeng
Liang, Wuying
Meng, Wenxin
Chen, Shuaijiang
Zhu, Yufan
Ye, Cundong
Jia, Kun
Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair
title Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair
title_full Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair
title_fullStr Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair
title_full_unstemmed Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair
title_short Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair
title_sort evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320857/
https://www.ncbi.nlm.nih.gov/pubmed/37415965
http://dx.doi.org/10.3389/fvets.2023.1162407
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