Cargando…
GBAP1 functions as a tumor promotor in hepatocellular carcinoma via the PI3K/AKT pathway
Hepatocellular carcinoma (HCC) is common worldwide, and novel therapeutic targets and biomarkers are needed to improve outcomes. In this study, bioinformatics analyses combined with in vitro and in vivo assays were used to identify the potential therapeutic targets. Differentially expressed genes (D...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320987/ https://www.ncbi.nlm.nih.gov/pubmed/37407932 http://dx.doi.org/10.1186/s12885-023-11107-7 |
_version_ | 1785068545085800448 |
---|---|
author | Chen, Rong Zhao, Meng An, Yanli Liu, Dongfang Tang, Qiusha |
author_facet | Chen, Rong Zhao, Meng An, Yanli Liu, Dongfang Tang, Qiusha |
author_sort | Chen, Rong |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) is common worldwide, and novel therapeutic targets and biomarkers are needed to improve outcomes. In this study, bioinformatics analyses combined with in vitro and in vivo assays were used to identify the potential therapeutic targets. Differentially expressed genes (DEG) in HCC were identified by the intersection between The Cancer Genome Atlas and International Cancer Genome Consortium data. The DEGs were evaluated by a gene set enrichment analysis as well as Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. A protein interaction network, univariate Cox regression, and Lasso regression were used to screen out hub genes correlated with survival. Increased expression of the long noncoding RNA GBAP1 in HCC was confirmed in additional datasets and its biological function was evaluated in HCC cell lines and nude mice. Among 121 DEGs, GBAP1 and PRC1 were identified as hub genes with significant prognostic value. Overexpression of GBAP1 in HCC was confirmed in 21 paired clinical tissues and liver cancer or normal cell lines. The inhibition of GBAP1 expression reduced HCC cell proliferation and promoted apoptosis by inactivating the PI3K/AKT pathway in vitro and in vivo. Therefore, GBAP1 has a pro-oncogenic function in HCC and is a candidate prognostic biomarker and therapeutic target. |
format | Online Article Text |
id | pubmed-10320987 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-103209872023-07-06 GBAP1 functions as a tumor promotor in hepatocellular carcinoma via the PI3K/AKT pathway Chen, Rong Zhao, Meng An, Yanli Liu, Dongfang Tang, Qiusha BMC Cancer Research Hepatocellular carcinoma (HCC) is common worldwide, and novel therapeutic targets and biomarkers are needed to improve outcomes. In this study, bioinformatics analyses combined with in vitro and in vivo assays were used to identify the potential therapeutic targets. Differentially expressed genes (DEG) in HCC were identified by the intersection between The Cancer Genome Atlas and International Cancer Genome Consortium data. The DEGs were evaluated by a gene set enrichment analysis as well as Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. A protein interaction network, univariate Cox regression, and Lasso regression were used to screen out hub genes correlated with survival. Increased expression of the long noncoding RNA GBAP1 in HCC was confirmed in additional datasets and its biological function was evaluated in HCC cell lines and nude mice. Among 121 DEGs, GBAP1 and PRC1 were identified as hub genes with significant prognostic value. Overexpression of GBAP1 in HCC was confirmed in 21 paired clinical tissues and liver cancer or normal cell lines. The inhibition of GBAP1 expression reduced HCC cell proliferation and promoted apoptosis by inactivating the PI3K/AKT pathway in vitro and in vivo. Therefore, GBAP1 has a pro-oncogenic function in HCC and is a candidate prognostic biomarker and therapeutic target. BioMed Central 2023-07-05 /pmc/articles/PMC10320987/ /pubmed/37407932 http://dx.doi.org/10.1186/s12885-023-11107-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Chen, Rong Zhao, Meng An, Yanli Liu, Dongfang Tang, Qiusha GBAP1 functions as a tumor promotor in hepatocellular carcinoma via the PI3K/AKT pathway |
title | GBAP1 functions as a tumor promotor in hepatocellular carcinoma via the PI3K/AKT pathway |
title_full | GBAP1 functions as a tumor promotor in hepatocellular carcinoma via the PI3K/AKT pathway |
title_fullStr | GBAP1 functions as a tumor promotor in hepatocellular carcinoma via the PI3K/AKT pathway |
title_full_unstemmed | GBAP1 functions as a tumor promotor in hepatocellular carcinoma via the PI3K/AKT pathway |
title_short | GBAP1 functions as a tumor promotor in hepatocellular carcinoma via the PI3K/AKT pathway |
title_sort | gbap1 functions as a tumor promotor in hepatocellular carcinoma via the pi3k/akt pathway |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320987/ https://www.ncbi.nlm.nih.gov/pubmed/37407932 http://dx.doi.org/10.1186/s12885-023-11107-7 |
work_keys_str_mv | AT chenrong gbap1functionsasatumorpromotorinhepatocellularcarcinomaviathepi3kaktpathway AT zhaomeng gbap1functionsasatumorpromotorinhepatocellularcarcinomaviathepi3kaktpathway AT anyanli gbap1functionsasatumorpromotorinhepatocellularcarcinomaviathepi3kaktpathway AT liudongfang gbap1functionsasatumorpromotorinhepatocellularcarcinomaviathepi3kaktpathway AT tangqiusha gbap1functionsasatumorpromotorinhepatocellularcarcinomaviathepi3kaktpathway |