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Hypoxia sensing requires H(2)S-dependent persulfidation of olfactory receptor 78
Oxygen (O(2)) sensing by the carotid body is critical for maintaining cardiorespiratory homeostasis during hypoxia. Hydrogen sulfide (H(2)S) signaling is implicated in carotid body activation by low O(2). Here, we show that persulfidation of olfactory receptor 78 (Olfr78) by H(2)S is an integral com...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321732/ https://www.ncbi.nlm.nih.gov/pubmed/37406126 http://dx.doi.org/10.1126/sciadv.adf3026 |
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author | Peng, Ying-Jie Nanduri, Jayasri Wang, Ning Kumar, Ganesh K. Bindokas, Vytautas Paul, Bindu D. Chen, Xuanmao Fox, Aaron P. Vignane, Thibaut Filipovic, Milos R. Prabhakar, Nanduri R. |
author_facet | Peng, Ying-Jie Nanduri, Jayasri Wang, Ning Kumar, Ganesh K. Bindokas, Vytautas Paul, Bindu D. Chen, Xuanmao Fox, Aaron P. Vignane, Thibaut Filipovic, Milos R. Prabhakar, Nanduri R. |
author_sort | Peng, Ying-Jie |
collection | PubMed |
description | Oxygen (O(2)) sensing by the carotid body is critical for maintaining cardiorespiratory homeostasis during hypoxia. Hydrogen sulfide (H(2)S) signaling is implicated in carotid body activation by low O(2). Here, we show that persulfidation of olfactory receptor 78 (Olfr78) by H(2)S is an integral component of carotid body activation by hypoxia. Hypoxia and H(2)S increased persulfidation in carotid body glomus cells and persulfidated cysteine(240) in Olfr78 protein in heterologous system. Olfr78 mutants manifest impaired carotid body sensory nerve, glomus cell, and breathing responses to H(2)S and hypoxia. Glomus cells are positive for G(Olf,) adenylate cyclase 3 (Adcy3) and cyclic nucleotide–gated channel alpha 2 (Cnga2), key molecules of odorant receptor signaling. Adcy3 or Cnga2 mutants exhibited impaired carotid body and glomus cell responses to H(2)S and breathing responses to hypoxia. These results suggest that H(2)S through redox modification of Olfr78 participates in carotid body activation by hypoxia to regulate breathing. |
format | Online Article Text |
id | pubmed-10321732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-103217322023-07-06 Hypoxia sensing requires H(2)S-dependent persulfidation of olfactory receptor 78 Peng, Ying-Jie Nanduri, Jayasri Wang, Ning Kumar, Ganesh K. Bindokas, Vytautas Paul, Bindu D. Chen, Xuanmao Fox, Aaron P. Vignane, Thibaut Filipovic, Milos R. Prabhakar, Nanduri R. Sci Adv Biomedicine and Life Sciences Oxygen (O(2)) sensing by the carotid body is critical for maintaining cardiorespiratory homeostasis during hypoxia. Hydrogen sulfide (H(2)S) signaling is implicated in carotid body activation by low O(2). Here, we show that persulfidation of olfactory receptor 78 (Olfr78) by H(2)S is an integral component of carotid body activation by hypoxia. Hypoxia and H(2)S increased persulfidation in carotid body glomus cells and persulfidated cysteine(240) in Olfr78 protein in heterologous system. Olfr78 mutants manifest impaired carotid body sensory nerve, glomus cell, and breathing responses to H(2)S and hypoxia. Glomus cells are positive for G(Olf,) adenylate cyclase 3 (Adcy3) and cyclic nucleotide–gated channel alpha 2 (Cnga2), key molecules of odorant receptor signaling. Adcy3 or Cnga2 mutants exhibited impaired carotid body and glomus cell responses to H(2)S and breathing responses to hypoxia. These results suggest that H(2)S through redox modification of Olfr78 participates in carotid body activation by hypoxia to regulate breathing. American Association for the Advancement of Science 2023-07-05 /pmc/articles/PMC10321732/ /pubmed/37406126 http://dx.doi.org/10.1126/sciadv.adf3026 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Peng, Ying-Jie Nanduri, Jayasri Wang, Ning Kumar, Ganesh K. Bindokas, Vytautas Paul, Bindu D. Chen, Xuanmao Fox, Aaron P. Vignane, Thibaut Filipovic, Milos R. Prabhakar, Nanduri R. Hypoxia sensing requires H(2)S-dependent persulfidation of olfactory receptor 78 |
title | Hypoxia sensing requires H(2)S-dependent persulfidation of olfactory receptor 78 |
title_full | Hypoxia sensing requires H(2)S-dependent persulfidation of olfactory receptor 78 |
title_fullStr | Hypoxia sensing requires H(2)S-dependent persulfidation of olfactory receptor 78 |
title_full_unstemmed | Hypoxia sensing requires H(2)S-dependent persulfidation of olfactory receptor 78 |
title_short | Hypoxia sensing requires H(2)S-dependent persulfidation of olfactory receptor 78 |
title_sort | hypoxia sensing requires h(2)s-dependent persulfidation of olfactory receptor 78 |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321732/ https://www.ncbi.nlm.nih.gov/pubmed/37406126 http://dx.doi.org/10.1126/sciadv.adf3026 |
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