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Russian collection of Brucella abortus vaccine strains: annotation, implementation and genomic analysis

Over the past 10 years, immunization of cattle in Russia has been performed using vaccines from Brucella abortus strains 82, 19 and 75/79. To prevent brucellosis in small ruminants, two vaccines have been used, from the Brucella melitensis strain REV-1 and the B. abortus strain 19; note that twice a...

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Autores principales: Prasolova, Olga, Krylova, Ekaterina, Bogomazova, Alexandra, Soltynskaya, Irina, Sklyarov, Oleg, Gordeeva, Veronika, Timofeeva, Irina, Motorygin, Anton, Panin, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10322197/
https://www.ncbi.nlm.nih.gov/pubmed/37415963
http://dx.doi.org/10.3389/fvets.2023.1154520
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author Prasolova, Olga
Krylova, Ekaterina
Bogomazova, Alexandra
Soltynskaya, Irina
Sklyarov, Oleg
Gordeeva, Veronika
Timofeeva, Irina
Motorygin, Anton
Panin, Alexander
author_facet Prasolova, Olga
Krylova, Ekaterina
Bogomazova, Alexandra
Soltynskaya, Irina
Sklyarov, Oleg
Gordeeva, Veronika
Timofeeva, Irina
Motorygin, Anton
Panin, Alexander
author_sort Prasolova, Olga
collection PubMed
description Over the past 10 years, immunization of cattle in Russia has been performed using vaccines from Brucella abortus strains 82, 19 and 75/79. To prevent brucellosis in small ruminants, two vaccines have been used, from the Brucella melitensis strain REV-1 and the B. abortus strain 19; note that twice as many animals have been immunized with the former vaccine than with the latter vaccine. The disadvantage of using these preparations is the formation of prolonged post-vaccination seropositivity, which is especially pronounced in animals after immunization with vaccines from B. abortus strain 19 and B. melitensis strain REV-1. This study aims to perform the whole genome sequencing of Brucella vaccine strains from the Russian collection. A bioinformatics analysis of the genomic data proved that the vaccine strains 75/79AB, 82, R-1096, and the KV 17/100 belong to ST-2, 104 M to ST-1, KV 13/100 to ST-5. This analysis allowed us to characterize vaccine strains’s phylogenetic relationships and to prove the close relation of vaccine strains 75/79AB, 82, R-1096. Also, we defined candidate mutations in genes pmm, wbdA, wbkA, wboA, and eryB, which could be responsible for the attenuated virulence of vaccine strains. The complete genomic sequences of B. abortus strains make further studies of bacterial pathogenicity determinants and virulence phenotype feasible, as well as their use in quality control of animal medicines.
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spelling pubmed-103221972023-07-06 Russian collection of Brucella abortus vaccine strains: annotation, implementation and genomic analysis Prasolova, Olga Krylova, Ekaterina Bogomazova, Alexandra Soltynskaya, Irina Sklyarov, Oleg Gordeeva, Veronika Timofeeva, Irina Motorygin, Anton Panin, Alexander Front Vet Sci Veterinary Science Over the past 10 years, immunization of cattle in Russia has been performed using vaccines from Brucella abortus strains 82, 19 and 75/79. To prevent brucellosis in small ruminants, two vaccines have been used, from the Brucella melitensis strain REV-1 and the B. abortus strain 19; note that twice as many animals have been immunized with the former vaccine than with the latter vaccine. The disadvantage of using these preparations is the formation of prolonged post-vaccination seropositivity, which is especially pronounced in animals after immunization with vaccines from B. abortus strain 19 and B. melitensis strain REV-1. This study aims to perform the whole genome sequencing of Brucella vaccine strains from the Russian collection. A bioinformatics analysis of the genomic data proved that the vaccine strains 75/79AB, 82, R-1096, and the KV 17/100 belong to ST-2, 104 M to ST-1, KV 13/100 to ST-5. This analysis allowed us to characterize vaccine strains’s phylogenetic relationships and to prove the close relation of vaccine strains 75/79AB, 82, R-1096. Also, we defined candidate mutations in genes pmm, wbdA, wbkA, wboA, and eryB, which could be responsible for the attenuated virulence of vaccine strains. The complete genomic sequences of B. abortus strains make further studies of bacterial pathogenicity determinants and virulence phenotype feasible, as well as their use in quality control of animal medicines. Frontiers Media S.A. 2023-06-21 /pmc/articles/PMC10322197/ /pubmed/37415963 http://dx.doi.org/10.3389/fvets.2023.1154520 Text en Copyright © 2023 Prasolova, Krylova, Bogomazova, Soltynskaya, Sklyarov, Gordeeva, Timofeeva, Motorygin and Panin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Prasolova, Olga
Krylova, Ekaterina
Bogomazova, Alexandra
Soltynskaya, Irina
Sklyarov, Oleg
Gordeeva, Veronika
Timofeeva, Irina
Motorygin, Anton
Panin, Alexander
Russian collection of Brucella abortus vaccine strains: annotation, implementation and genomic analysis
title Russian collection of Brucella abortus vaccine strains: annotation, implementation and genomic analysis
title_full Russian collection of Brucella abortus vaccine strains: annotation, implementation and genomic analysis
title_fullStr Russian collection of Brucella abortus vaccine strains: annotation, implementation and genomic analysis
title_full_unstemmed Russian collection of Brucella abortus vaccine strains: annotation, implementation and genomic analysis
title_short Russian collection of Brucella abortus vaccine strains: annotation, implementation and genomic analysis
title_sort russian collection of brucella abortus vaccine strains: annotation, implementation and genomic analysis
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10322197/
https://www.ncbi.nlm.nih.gov/pubmed/37415963
http://dx.doi.org/10.3389/fvets.2023.1154520
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