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Protocol for immunofluorescence staining of murine helminth-infected intestinal and lung tissues
Preserving structurally intact tissue through proper tissue fixation and cryopreservation minimizes tissue autolysis, desiccation, and enzymatic degradation. In this protocol, we describe the use of an optimized fresh freezing technique that cryopreserves the tissue and favors the retention of the n...
Autores principales: | El-Naccache, Darine W., Chen, Fei, Gause, William C. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10322902/ https://www.ncbi.nlm.nih.gov/pubmed/37086410 http://dx.doi.org/10.1016/j.xpro.2023.102208 |
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