Standardized immunoprecipitation protocol for efficient isolation of native apolipoprotein E particles utilizing HJ15.4 monoclonal antibody

The apolipoprotein E protein (apoE) confers differential risk for Alzheimer’s disease depending on which isoforms are expressed. Here, we present a 2-day immunoprecipitation protocol using the HJ15.4 monoclonal apoE antibody for the pull-down of native apoE particles. We describe major steps for apo...

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Detalles Bibliográficos
Autores principales: O’Leary, Justin, Raulin, Ana-Caroline, Li, Zonghua, Martens, Yuka, Inoue, Yasuteru, Strickland, Michael R., Han, Xianlin, Holtzman, David M., Bu, Guojun, Zhao, Na
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323120/
https://www.ncbi.nlm.nih.gov/pubmed/37289593
http://dx.doi.org/10.1016/j.xpro.2023.102271

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