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Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde

BACKGROUND: Sleep disorders are accompanied by increased anxiety and somatic pain. In addition, it has been observed that anxiety and pain have a boosting effect on each other, resulting in continued sleep disturbances. Amygdala's (CeA) central nucleus plays a crucial role in these processes. C...

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Autores principales: Hadeiy, Seyed Kaveh, Habtemariam, Solomon, Shankayi, Zeinab, Shahyad, Shima, Sahraei, Hedayat, Asghardoust Rezaei, Milad, Bahrami, Farideh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323214/
https://www.ncbi.nlm.nih.gov/pubmed/37424741
http://dx.doi.org/10.1016/j.sleepx.2023.100069
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author Hadeiy, Seyed Kaveh
Habtemariam, Solomon
Shankayi, Zeinab
Shahyad, Shima
Sahraei, Hedayat
Asghardoust Rezaei, Milad
Bahrami, Farideh
author_facet Hadeiy, Seyed Kaveh
Habtemariam, Solomon
Shankayi, Zeinab
Shahyad, Shima
Sahraei, Hedayat
Asghardoust Rezaei, Milad
Bahrami, Farideh
author_sort Hadeiy, Seyed Kaveh
collection PubMed
description BACKGROUND: Sleep disorders are accompanied by increased anxiety and somatic pain. In addition, it has been observed that anxiety and pain have a boosting effect on each other, resulting in continued sleep disturbances. Amygdala's (CeA) central nucleus plays a crucial role in these processes. Cinnamaldehyde (Cinn) is an aromatic compound with anti-anxiety, antioxidant, and sleep-promoting properties. The present study uses sleep-deprived rats to examine the effects of an intra-CeA injection of Cinn on pain and anxiety. METHODS: Sleep deprivation (SD) was induced using the platform technique. 35 male Wistar rats were divided into five groups. Anxiety state and nociception were evaluated among groups using formalin test (F.T.), open field test (OFT), and elevated plus maze (EPM). Anxiety tests (OFT and EPM) were conducted in all groups. The first group was undergone FT without induction of SD (SD(−)FT(+)). The second group received SD without FT(SD(+)FT(−)). The third group received both SD and FT(SD(+)FT(+)). The treatment and vehicle groups have undergone both SD and FT in addition to the respectively intra-CeA injection of Cinn (SD(+)FT(+) Cinn) and Cinn vehicle (SD(+)FT(+) VC). The recorded behaviors were analyzed between groups using IBM SPSS 24th version. RESULTS: SD did not lead to any significant difference in nociceptive behaviors in FT between groups SD(−)FT(+) and SD(+)FT(+) (P ≥ 0.05). At the same time, there was a considerable discrepancy in rearing behaviors (P < 0.006) and the number of fecal boli (P < 0.004) recorded in OFM between these groups. Treatment with Cinn led to decreased nociception (P < 0.038), decreased rearing behaviors (P < 0.01), and reduced defecation (P < 0.004) in group SD + FT+ Cinn in comparison to the group SD(+)FT(+). There were no differences in anxiety test results between the first and second groups (P ≥ 0.05). CONCLUSION: SD can lead to elevated anxiety, while intra-CeA injection of Cinn ameliorated both perceptions of acute pain and anxiety. Besides, the conduction of FT before the anxiety test led to no disturbance in the results of anxiety tests.
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spelling pubmed-103232142023-07-07 Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde Hadeiy, Seyed Kaveh Habtemariam, Solomon Shankayi, Zeinab Shahyad, Shima Sahraei, Hedayat Asghardoust Rezaei, Milad Bahrami, Farideh Sleep Med X Article BACKGROUND: Sleep disorders are accompanied by increased anxiety and somatic pain. In addition, it has been observed that anxiety and pain have a boosting effect on each other, resulting in continued sleep disturbances. Amygdala's (CeA) central nucleus plays a crucial role in these processes. Cinnamaldehyde (Cinn) is an aromatic compound with anti-anxiety, antioxidant, and sleep-promoting properties. The present study uses sleep-deprived rats to examine the effects of an intra-CeA injection of Cinn on pain and anxiety. METHODS: Sleep deprivation (SD) was induced using the platform technique. 35 male Wistar rats were divided into five groups. Anxiety state and nociception were evaluated among groups using formalin test (F.T.), open field test (OFT), and elevated plus maze (EPM). Anxiety tests (OFT and EPM) were conducted in all groups. The first group was undergone FT without induction of SD (SD(−)FT(+)). The second group received SD without FT(SD(+)FT(−)). The third group received both SD and FT(SD(+)FT(+)). The treatment and vehicle groups have undergone both SD and FT in addition to the respectively intra-CeA injection of Cinn (SD(+)FT(+) Cinn) and Cinn vehicle (SD(+)FT(+) VC). The recorded behaviors were analyzed between groups using IBM SPSS 24th version. RESULTS: SD did not lead to any significant difference in nociceptive behaviors in FT between groups SD(−)FT(+) and SD(+)FT(+) (P ≥ 0.05). At the same time, there was a considerable discrepancy in rearing behaviors (P < 0.006) and the number of fecal boli (P < 0.004) recorded in OFM between these groups. Treatment with Cinn led to decreased nociception (P < 0.038), decreased rearing behaviors (P < 0.01), and reduced defecation (P < 0.004) in group SD + FT+ Cinn in comparison to the group SD(+)FT(+). There were no differences in anxiety test results between the first and second groups (P ≥ 0.05). CONCLUSION: SD can lead to elevated anxiety, while intra-CeA injection of Cinn ameliorated both perceptions of acute pain and anxiety. Besides, the conduction of FT before the anxiety test led to no disturbance in the results of anxiety tests. Elsevier 2023-03-23 /pmc/articles/PMC10323214/ /pubmed/37424741 http://dx.doi.org/10.1016/j.sleepx.2023.100069 Text en © 2023 Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Hadeiy, Seyed Kaveh
Habtemariam, Solomon
Shankayi, Zeinab
Shahyad, Shima
Sahraei, Hedayat
Asghardoust Rezaei, Milad
Bahrami, Farideh
Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde
title Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde
title_full Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde
title_fullStr Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde
title_full_unstemmed Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde
title_short Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde
title_sort amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323214/
https://www.ncbi.nlm.nih.gov/pubmed/37424741
http://dx.doi.org/10.1016/j.sleepx.2023.100069
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