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Th2 cells infiltrating high-grade serous ovarian cancer: a feature that may account for the poor prognosis

OBJECTIVE: We aimed to investigate the differences of transcriptome profile between 2 groups of high-grade serous ovarian cancer (HGSOC) patients with distinct outcomes and identify potential biomarkers for recurrence. METHODS: RNA sequencing was performed in 2 groups of HGSOC patients with similar...

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Autores principales: Su, Hao, Jin, Yueqi, Tao, Changyu, Yang, Hua, Yang, Ence, Zhang, Wei-Guang, Feng, Fengzhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asian Society of Gynecologic Oncology; Korean Society of Gynecologic Oncology; Japan Society of Gynecologic Oncology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323303/
https://www.ncbi.nlm.nih.gov/pubmed/36998223
http://dx.doi.org/10.3802/jgo.2023.34.e48
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author Su, Hao
Jin, Yueqi
Tao, Changyu
Yang, Hua
Yang, Ence
Zhang, Wei-Guang
Feng, Fengzhi
author_facet Su, Hao
Jin, Yueqi
Tao, Changyu
Yang, Hua
Yang, Ence
Zhang, Wei-Guang
Feng, Fengzhi
author_sort Su, Hao
collection PubMed
description OBJECTIVE: We aimed to investigate the differences of transcriptome profile between 2 groups of high-grade serous ovarian cancer (HGSOC) patients with distinct outcomes and identify potential biomarkers for recurrence. METHODS: RNA sequencing was performed in 2 groups of HGSOC patients with similar demographic characteristics but exhibiting distinct progression-free survival (PFS). Transcriptome data of poor response (PR; PFS ≤6 months) and good response (GR; PFS ≥12 months) group were compared. We employed xCell to evaluate the abundance of 63 cells in tumor microenvironment. The predictive value of recurrence-related tumor infiltration cells was validated in cohort data from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) dataset. The weighted correlation network analysis was performed to identify the genes related to cell infiltration. RESULTS: PR patients exhibited a distinct tumor infiltration immune cells-related transcriptional profile compared to GR patients, such as lower signatures of leukocyte differentiation, activation and chemotaxis. The fraction of T-helper 2 (Th2) cells infiltration was significantly higher in PR group than in GR group. High infiltration of Th2 was significantly associated with unfavorable prognosis in the GEO cohort (area under the curve=0.84 at 6 months recurrence) and TCGA cohort (p=0.008). Genes enriched to extracellular matrix organization and integrin binding were relevant to Th2 infiltration. CONCLUSION: Patients with HGSOC having shorter PFS exhibited a distinct gene signature that related to tumor-infiltrating immune cells. The level of Th2 infiltration could facilitate patient recurrence risk stratification and may be a promising biomarker for prognosis prediction and immune-related treatment.
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spelling pubmed-103233032023-07-07 Th2 cells infiltrating high-grade serous ovarian cancer: a feature that may account for the poor prognosis Su, Hao Jin, Yueqi Tao, Changyu Yang, Hua Yang, Ence Zhang, Wei-Guang Feng, Fengzhi J Gynecol Oncol Original Article OBJECTIVE: We aimed to investigate the differences of transcriptome profile between 2 groups of high-grade serous ovarian cancer (HGSOC) patients with distinct outcomes and identify potential biomarkers for recurrence. METHODS: RNA sequencing was performed in 2 groups of HGSOC patients with similar demographic characteristics but exhibiting distinct progression-free survival (PFS). Transcriptome data of poor response (PR; PFS ≤6 months) and good response (GR; PFS ≥12 months) group were compared. We employed xCell to evaluate the abundance of 63 cells in tumor microenvironment. The predictive value of recurrence-related tumor infiltration cells was validated in cohort data from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) dataset. The weighted correlation network analysis was performed to identify the genes related to cell infiltration. RESULTS: PR patients exhibited a distinct tumor infiltration immune cells-related transcriptional profile compared to GR patients, such as lower signatures of leukocyte differentiation, activation and chemotaxis. The fraction of T-helper 2 (Th2) cells infiltration was significantly higher in PR group than in GR group. High infiltration of Th2 was significantly associated with unfavorable prognosis in the GEO cohort (area under the curve=0.84 at 6 months recurrence) and TCGA cohort (p=0.008). Genes enriched to extracellular matrix organization and integrin binding were relevant to Th2 infiltration. CONCLUSION: Patients with HGSOC having shorter PFS exhibited a distinct gene signature that related to tumor-infiltrating immune cells. The level of Th2 infiltration could facilitate patient recurrence risk stratification and may be a promising biomarker for prognosis prediction and immune-related treatment. Asian Society of Gynecologic Oncology; Korean Society of Gynecologic Oncology; Japan Society of Gynecologic Oncology 2023-03-23 /pmc/articles/PMC10323303/ /pubmed/36998223 http://dx.doi.org/10.3802/jgo.2023.34.e48 Text en © 2023. Asian Society of Gynecologic Oncology, Korean Society of Gynecologic Oncology, and Japan Society of Gynecologic Oncology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Su, Hao
Jin, Yueqi
Tao, Changyu
Yang, Hua
Yang, Ence
Zhang, Wei-Guang
Feng, Fengzhi
Th2 cells infiltrating high-grade serous ovarian cancer: a feature that may account for the poor prognosis
title Th2 cells infiltrating high-grade serous ovarian cancer: a feature that may account for the poor prognosis
title_full Th2 cells infiltrating high-grade serous ovarian cancer: a feature that may account for the poor prognosis
title_fullStr Th2 cells infiltrating high-grade serous ovarian cancer: a feature that may account for the poor prognosis
title_full_unstemmed Th2 cells infiltrating high-grade serous ovarian cancer: a feature that may account for the poor prognosis
title_short Th2 cells infiltrating high-grade serous ovarian cancer: a feature that may account for the poor prognosis
title_sort th2 cells infiltrating high-grade serous ovarian cancer: a feature that may account for the poor prognosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323303/
https://www.ncbi.nlm.nih.gov/pubmed/36998223
http://dx.doi.org/10.3802/jgo.2023.34.e48
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