Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome

Staphylococcus aureus bacteraemia (SAB) is a major cause of blood-stream infection (BSI) in both healthcare and community settings. While the underlying comorbidities of a patient significantly contributes to their susceptibility to and outcome following SAB, recent studies show the importance of th...

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Autores principales: Brignoli, Tarcisio, Recker, Mario, Lee, Winnie W. Y., Dong, Tim, Bhamber, Ranjeet, Albur, Mahableshwar, Williams, Philip, Dowsey, Andrew W., Massey, Ruth C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2022
Materias:
Microbial Virulence and Pathogenesis
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323763/
https://www.ncbi.nlm.nih.gov/pubmed/35997594
http://dx.doi.org/10.1099/mic.0.001223
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author Brignoli, Tarcisio
Recker, Mario
Lee, Winnie W. Y.
Dong, Tim
Bhamber, Ranjeet
Albur, Mahableshwar
Williams, Philip
Dowsey, Andrew W.
Massey, Ruth C.
author_facet Brignoli, Tarcisio
Recker, Mario
Lee, Winnie W. Y.
Dong, Tim
Bhamber, Ranjeet
Albur, Mahableshwar
Williams, Philip
Dowsey, Andrew W.
Massey, Ruth C.
author_sort Brignoli, Tarcisio
collection PubMed
description Staphylococcus aureus bacteraemia (SAB) is a major cause of blood-stream infection (BSI) in both healthcare and community settings. While the underlying comorbidities of a patient significantly contributes to their susceptibility to and outcome following SAB, recent studies show the importance of the level of cytolytic toxin production by the infecting bacterium. In this study we demonstrate that this cytotoxicity can be determined directly from the diagnostic MALDI-TOF mass spectrum generated in a routine diagnostic laboratory. With further development this information could be used to guide the management and improve the outcomes for SAB patients.
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spelling pubmed-103237632023-07-07 Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome Brignoli, Tarcisio Recker, Mario Lee, Winnie W. Y. Dong, Tim Bhamber, Ranjeet Albur, Mahableshwar Williams, Philip Dowsey, Andrew W. Massey, Ruth C. Microbiology (Reading) Microbial Virulence and Pathogenesis Staphylococcus aureus bacteraemia (SAB) is a major cause of blood-stream infection (BSI) in both healthcare and community settings. While the underlying comorbidities of a patient significantly contributes to their susceptibility to and outcome following SAB, recent studies show the importance of the level of cytolytic toxin production by the infecting bacterium. In this study we demonstrate that this cytotoxicity can be determined directly from the diagnostic MALDI-TOF mass spectrum generated in a routine diagnostic laboratory. With further development this information could be used to guide the management and improve the outcomes for SAB patients. Microbiology Society 2022-08-23 /pmc/articles/PMC10323763/ /pubmed/35997594 http://dx.doi.org/10.1099/mic.0.001223 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
spellingShingle Microbial Virulence and Pathogenesis
Brignoli, Tarcisio
Recker, Mario
Lee, Winnie W. Y.
Dong, Tim
Bhamber, Ranjeet
Albur, Mahableshwar
Williams, Philip
Dowsey, Andrew W.
Massey, Ruth C.
Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome
title Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome
title_full Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome
title_fullStr Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome
title_full_unstemmed Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome
title_short Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome
title_sort diagnostic maldi-tof ms can differentiate between high and low toxic staphylococcus aureus bacteraemia isolates as a predictor of patient outcome
topic Microbial Virulence and Pathogenesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323763/
https://www.ncbi.nlm.nih.gov/pubmed/35997594
http://dx.doi.org/10.1099/mic.0.001223
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