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Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism

[Image: see text] Distinguishing isomeric saccharides poses a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC–MS). In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characteriz...

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Autores principales: van Outersterp, Rianne E., Kooijman, Pieter C., Merx, Jona, Engelke, Udo F.H., Omidikia, Nematollah, Tonneijck, Mei-Lan H., Houthuijs, Kas J., Berden, Giel, Peters, Tessa M.A., Lefeber, Dirk J., Willemsen, Michel A. A. P., Mecinovic, Jasmin, Jansen, Jeroen J., Coene, Karlien L.M., Wevers, Ron A., Boltje, Thomas J., Oomens, Jos, Martens, Jonathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323868/
https://www.ncbi.nlm.nih.gov/pubmed/37341384
http://dx.doi.org/10.1021/acs.analchem.3c00363
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author van Outersterp, Rianne E.
Kooijman, Pieter C.
Merx, Jona
Engelke, Udo F.H.
Omidikia, Nematollah
Tonneijck, Mei-Lan H.
Houthuijs, Kas J.
Berden, Giel
Peters, Tessa M.A.
Lefeber, Dirk J.
Willemsen, Michel A. A. P.
Mecinovic, Jasmin
Jansen, Jeroen J.
Coene, Karlien L.M.
Wevers, Ron A.
Boltje, Thomas J.
Oomens, Jos
Martens, Jonathan
author_facet van Outersterp, Rianne E.
Kooijman, Pieter C.
Merx, Jona
Engelke, Udo F.H.
Omidikia, Nematollah
Tonneijck, Mei-Lan H.
Houthuijs, Kas J.
Berden, Giel
Peters, Tessa M.A.
Lefeber, Dirk J.
Willemsen, Michel A. A. P.
Mecinovic, Jasmin
Jansen, Jeroen J.
Coene, Karlien L.M.
Wevers, Ron A.
Boltje, Thomas J.
Oomens, Jos
Martens, Jonathan
author_sort van Outersterp, Rianne E.
collection PubMed
description [Image: see text] Distinguishing isomeric saccharides poses a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC–MS). In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value. Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300–1000 cm(–1)) provide well-resolved and highly diagnostic features. We show that this enables distinction of isomeric saccharides that differ either by their composition of monosaccharide units and/or the orientation of their glycosidic linkages. We demonstrate the utility of this approach from single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage. Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.
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spelling pubmed-103238682023-07-07 Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism van Outersterp, Rianne E. Kooijman, Pieter C. Merx, Jona Engelke, Udo F.H. Omidikia, Nematollah Tonneijck, Mei-Lan H. Houthuijs, Kas J. Berden, Giel Peters, Tessa M.A. Lefeber, Dirk J. Willemsen, Michel A. A. P. Mecinovic, Jasmin Jansen, Jeroen J. Coene, Karlien L.M. Wevers, Ron A. Boltje, Thomas J. Oomens, Jos Martens, Jonathan Anal Chem [Image: see text] Distinguishing isomeric saccharides poses a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC–MS). In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value. Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300–1000 cm(–1)) provide well-resolved and highly diagnostic features. We show that this enables distinction of isomeric saccharides that differ either by their composition of monosaccharide units and/or the orientation of their glycosidic linkages. We demonstrate the utility of this approach from single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage. Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices. American Chemical Society 2023-06-21 /pmc/articles/PMC10323868/ /pubmed/37341384 http://dx.doi.org/10.1021/acs.analchem.3c00363 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle van Outersterp, Rianne E.
Kooijman, Pieter C.
Merx, Jona
Engelke, Udo F.H.
Omidikia, Nematollah
Tonneijck, Mei-Lan H.
Houthuijs, Kas J.
Berden, Giel
Peters, Tessa M.A.
Lefeber, Dirk J.
Willemsen, Michel A. A. P.
Mecinovic, Jasmin
Jansen, Jeroen J.
Coene, Karlien L.M.
Wevers, Ron A.
Boltje, Thomas J.
Oomens, Jos
Martens, Jonathan
Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism
title Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism
title_full Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism
title_fullStr Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism
title_full_unstemmed Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism
title_short Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism
title_sort distinguishing oligosaccharide isomers using far-infrared ion spectroscopy: identification of biomarkers for inborn errors of metabolism
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323868/
https://www.ncbi.nlm.nih.gov/pubmed/37341384
http://dx.doi.org/10.1021/acs.analchem.3c00363
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