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MicroRNA‐128 suppresses tau phosphorylation and reduces amyloid‐beta accumulation by inhibiting the expression of GSK3β, APPBP2, and mTOR in Alzheimer's disease
INTRODUCTION AND AIMS: Alzheimer's disease (AD) is characterized by the abnormal accumulation of hyperphosphorylated tau proteins and amyloid‐beta (Aβ) peptides. Recent studies have shown that many microRNAs (miRNAs) are dysregulated in AD, and modulation of these miRNAs can influence the devel...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10324361/ https://www.ncbi.nlm.nih.gov/pubmed/36880288 http://dx.doi.org/10.1111/cns.14143 |
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author | Li, Siwen Poon, Chi Him Zhang, Zhigang Yue, Ming Chen, Ruijun Zhang, Yalun Hossain, Md. Farhad Pan, Yining Zhao, Jun Rong, Lei Chu, Leung Wing Shea, Yat Fung Rogaeva, Ekaterina Tu, Jie St George‐Hyslop, Peter Lim, Lee Wei Song, You‐Qiang |
author_facet | Li, Siwen Poon, Chi Him Zhang, Zhigang Yue, Ming Chen, Ruijun Zhang, Yalun Hossain, Md. Farhad Pan, Yining Zhao, Jun Rong, Lei Chu, Leung Wing Shea, Yat Fung Rogaeva, Ekaterina Tu, Jie St George‐Hyslop, Peter Lim, Lee Wei Song, You‐Qiang |
author_sort | Li, Siwen |
collection | PubMed |
description | INTRODUCTION AND AIMS: Alzheimer's disease (AD) is characterized by the abnormal accumulation of hyperphosphorylated tau proteins and amyloid‐beta (Aβ) peptides. Recent studies have shown that many microRNAs (miRNAs) are dysregulated in AD, and modulation of these miRNAs can influence the development of tau and Aβ pathology. The brain‐specific miRNA miR‐128, encoded by MIR128‐1 and MIR128‐2, is important for brain development and dysregulated in AD. In this study, the role of miR‐128 in tau and Aβ pathology as well as the regulatory mechanism underlying its dysregulation were investigated. METHODS: The effect of miR‐128 on tau phosphorylation and Aβ accumulation was examined in AD cellular models through miR‐128 overexpression and inhibition. The therapeutic potential of miR‐128 in AD mouse model was assessed by comparing phenotypes of 5XFAD mice administered with miR‐128‐expressing AAVs with 5XFAD mice administered with control AAVs. Phenotypes examined included behavior, plaque load, and protein expression. The regulatory factor of miR‐128 transcription was identified through luciferase reporter assay and validated by siRNA knockdown and ChIP analysis. RESULTS: Both gain‐of‐function and loss‐of‐function studies in AD cellular models reveal that miR‐128 represses tau phosphorylation and Aβ secretion. Subsequent investigations show that miR‐128 directly inhibits the expression of tau phosphorylation kinase GSK3β and Aβ modulators APPBP2 and mTOR. Upregulation of miR‐128 in the hippocampus of 5XFAD mice ameliorates learning and memory impairments, decreases plaque deposition, and enhances autophagic flux. We further demonstrated that C/EBPα transactivates MIR128‐1 transcription, while both C/EBPα and miR‐128 expression are inhibited by Aβ. CONCLUSION: Our findings suggest that miR‐128 suppresses AD pathogenesis, and could be a promising therapeutic target for AD. We also find a possible mechanism underlying the dysregulation of miR‐128 in AD, in which Aβ reduces miR‐128 expression by inhibiting C/EBPα. |
format | Online Article Text |
id | pubmed-10324361 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103243612023-07-07 MicroRNA‐128 suppresses tau phosphorylation and reduces amyloid‐beta accumulation by inhibiting the expression of GSK3β, APPBP2, and mTOR in Alzheimer's disease Li, Siwen Poon, Chi Him Zhang, Zhigang Yue, Ming Chen, Ruijun Zhang, Yalun Hossain, Md. Farhad Pan, Yining Zhao, Jun Rong, Lei Chu, Leung Wing Shea, Yat Fung Rogaeva, Ekaterina Tu, Jie St George‐Hyslop, Peter Lim, Lee Wei Song, You‐Qiang CNS Neurosci Ther Original Articles INTRODUCTION AND AIMS: Alzheimer's disease (AD) is characterized by the abnormal accumulation of hyperphosphorylated tau proteins and amyloid‐beta (Aβ) peptides. Recent studies have shown that many microRNAs (miRNAs) are dysregulated in AD, and modulation of these miRNAs can influence the development of tau and Aβ pathology. The brain‐specific miRNA miR‐128, encoded by MIR128‐1 and MIR128‐2, is important for brain development and dysregulated in AD. In this study, the role of miR‐128 in tau and Aβ pathology as well as the regulatory mechanism underlying its dysregulation were investigated. METHODS: The effect of miR‐128 on tau phosphorylation and Aβ accumulation was examined in AD cellular models through miR‐128 overexpression and inhibition. The therapeutic potential of miR‐128 in AD mouse model was assessed by comparing phenotypes of 5XFAD mice administered with miR‐128‐expressing AAVs with 5XFAD mice administered with control AAVs. Phenotypes examined included behavior, plaque load, and protein expression. The regulatory factor of miR‐128 transcription was identified through luciferase reporter assay and validated by siRNA knockdown and ChIP analysis. RESULTS: Both gain‐of‐function and loss‐of‐function studies in AD cellular models reveal that miR‐128 represses tau phosphorylation and Aβ secretion. Subsequent investigations show that miR‐128 directly inhibits the expression of tau phosphorylation kinase GSK3β and Aβ modulators APPBP2 and mTOR. Upregulation of miR‐128 in the hippocampus of 5XFAD mice ameliorates learning and memory impairments, decreases plaque deposition, and enhances autophagic flux. We further demonstrated that C/EBPα transactivates MIR128‐1 transcription, while both C/EBPα and miR‐128 expression are inhibited by Aβ. CONCLUSION: Our findings suggest that miR‐128 suppresses AD pathogenesis, and could be a promising therapeutic target for AD. We also find a possible mechanism underlying the dysregulation of miR‐128 in AD, in which Aβ reduces miR‐128 expression by inhibiting C/EBPα. John Wiley and Sons Inc. 2023-03-07 /pmc/articles/PMC10324361/ /pubmed/36880288 http://dx.doi.org/10.1111/cns.14143 Text en © 2023 The Authors. CNS Neuroscience & Therapeutics published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Li, Siwen Poon, Chi Him Zhang, Zhigang Yue, Ming Chen, Ruijun Zhang, Yalun Hossain, Md. Farhad Pan, Yining Zhao, Jun Rong, Lei Chu, Leung Wing Shea, Yat Fung Rogaeva, Ekaterina Tu, Jie St George‐Hyslop, Peter Lim, Lee Wei Song, You‐Qiang MicroRNA‐128 suppresses tau phosphorylation and reduces amyloid‐beta accumulation by inhibiting the expression of GSK3β, APPBP2, and mTOR in Alzheimer's disease |
title |
MicroRNA‐128 suppresses tau phosphorylation and reduces amyloid‐beta accumulation by inhibiting the expression of GSK3β, APPBP2, and mTOR in Alzheimer's disease |
title_full |
MicroRNA‐128 suppresses tau phosphorylation and reduces amyloid‐beta accumulation by inhibiting the expression of GSK3β, APPBP2, and mTOR in Alzheimer's disease |
title_fullStr |
MicroRNA‐128 suppresses tau phosphorylation and reduces amyloid‐beta accumulation by inhibiting the expression of GSK3β, APPBP2, and mTOR in Alzheimer's disease |
title_full_unstemmed |
MicroRNA‐128 suppresses tau phosphorylation and reduces amyloid‐beta accumulation by inhibiting the expression of GSK3β, APPBP2, and mTOR in Alzheimer's disease |
title_short |
MicroRNA‐128 suppresses tau phosphorylation and reduces amyloid‐beta accumulation by inhibiting the expression of GSK3β, APPBP2, and mTOR in Alzheimer's disease |
title_sort | microrna‐128 suppresses tau phosphorylation and reduces amyloid‐beta accumulation by inhibiting the expression of gsk3β, appbp2, and mtor in alzheimer's disease |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10324361/ https://www.ncbi.nlm.nih.gov/pubmed/36880288 http://dx.doi.org/10.1111/cns.14143 |
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