Crystal structures and kinetic studies of a laboratory evolved aldehyde reductase explain the dramatic shift of its new substrate specificity

The Fe(2+)-dependent E. coli enzyme FucO catalyzes the reversible interconversion of short-chain (S)-lactaldehyde and (S)-1,2-propane­diol, using NADH and NAD(+) as cofactors, respectively. Laboratory-directed evolution experiments have been carried out previously using phenyl­acetaldehyde as the su...

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Detalles Bibliográficos
Autores principales: Sridhar, Shruthi, Zavarise, Alberto, Kiema, Tiila-Riikka, Dalwani, Subhadra, Eriksson, Tor, Hajee, Yannick, Reddy Enugala, Thilak, Wierenga, Rik K., Widersten, Mikael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2023
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10324492/
https://www.ncbi.nlm.nih.gov/pubmed/37261425
http://dx.doi.org/10.1107/S205225252300444X

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10324492/
https://www.ncbi.nlm.nih.gov/pubmed/37261425
http://dx.doi.org/10.1107/S205225252300444X

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